Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats
Objective Dapsone (diaminodiphenyl sulfone, DDS) is currently used to treat leprosy, malaria, dermatitis herpetiformis, and other diseases. It is also used to treat pneumocystis pneumonia and Toxoplasma gondii infection in HIV-positive patients. The most common adverse effect of DDS is methemoglobin...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Korean Society of Emergency Medicine
2018-09-01
|
| Series: | Clinical and Experimental Emergency Medicine |
| Subjects: | |
| Online Access: | http://www.ceemjournal.org/upload/pdf/ceem-17-253.pdf |
| _version_ | 1797895595123277824 |
|---|---|
| author | Changwoo Kang Dong Hoon Kim Taeyun Kim Soo Hoon Lee Jin Hee Jeong Sang Bong Lee Jin Hyun Kim Myeong Hee Jung Kyung-woo Lee In Sung Park |
| author_facet | Changwoo Kang Dong Hoon Kim Taeyun Kim Soo Hoon Lee Jin Hee Jeong Sang Bong Lee Jin Hyun Kim Myeong Hee Jung Kyung-woo Lee In Sung Park |
| author_sort | Changwoo Kang |
| collection | DOAJ |
| description | Objective Dapsone (diaminodiphenyl sulfone, DDS) is currently used to treat leprosy, malaria, dermatitis herpetiformis, and other diseases. It is also used to treat pneumocystis pneumonia and Toxoplasma gondii infection in HIV-positive patients. The most common adverse effect of DDS is methemoglobinemia from oxidative stress. Ascorbic acid is an antioxidant and reducing agent that scavenges the free radicals produced by oxidative stress. The present study aimed to investigate the effect of ascorbic acid in the treatment of DDS induced methemoglobinemia. Methods Male Sprague-Dawley rats were divided into three groups: an ascorbic acid group, a methylene blue (MB) group, and a control group. After DDS (40 mg/kg) treatment via oral gavage, ascorbic acid (15 mg/kg), MB (1 mg/kg), or normal saline were administered via tail vein injection. Depending on the duration of the DDS treatment, blood methemoglobin levels, as well as the nitric oxide levels and catalase activity, were measured at 60, 120, or 180 minutes after DDS administration. Results Methemoglobin concentrations in the ascorbic acid and MB groups were significantly lower compared to those in the control group across multiple time points. The plasma nitric oxide levels and catalase activity were not different among the groups or time points. Conclusion Intravenous ascorbic acid administration is effective in treating DDS-induced methemoglobinemia in a murine model. |
| first_indexed | 2024-04-10T07:29:26Z |
| format | Article |
| id | doaj.art-116c2b304e434fb08c50d3cd27c122aa |
| institution | Directory Open Access Journal |
| issn | 2383-4625 |
| language | English |
| last_indexed | 2024-04-10T07:29:26Z |
| publishDate | 2018-09-01 |
| publisher | The Korean Society of Emergency Medicine |
| record_format | Article |
| series | Clinical and Experimental Emergency Medicine |
| spelling | doaj.art-116c2b304e434fb08c50d3cd27c122aa2023-02-24T01:32:12ZengThe Korean Society of Emergency MedicineClinical and Experimental Emergency Medicine2383-46252018-09-015319219810.15441/ceem.17.253195Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in ratsChangwoo Kang0Dong Hoon Kim1Taeyun Kim2Soo Hoon Lee3Jin Hee Jeong4Sang Bong Lee5Jin Hyun Kim6Myeong Hee Jung7Kyung-woo Lee8In Sung Park9 Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea Institute of Health Sciences, Gyeongsang National University, Jinju, Korea Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Korea Department of Emergency Medicine, Catholic University of Daegu, Daegu, Korea Institute of Health Sciences, Gyeongsang National University, Jinju, KoreaObjective Dapsone (diaminodiphenyl sulfone, DDS) is currently used to treat leprosy, malaria, dermatitis herpetiformis, and other diseases. It is also used to treat pneumocystis pneumonia and Toxoplasma gondii infection in HIV-positive patients. The most common adverse effect of DDS is methemoglobinemia from oxidative stress. Ascorbic acid is an antioxidant and reducing agent that scavenges the free radicals produced by oxidative stress. The present study aimed to investigate the effect of ascorbic acid in the treatment of DDS induced methemoglobinemia. Methods Male Sprague-Dawley rats were divided into three groups: an ascorbic acid group, a methylene blue (MB) group, and a control group. After DDS (40 mg/kg) treatment via oral gavage, ascorbic acid (15 mg/kg), MB (1 mg/kg), or normal saline were administered via tail vein injection. Depending on the duration of the DDS treatment, blood methemoglobin levels, as well as the nitric oxide levels and catalase activity, were measured at 60, 120, or 180 minutes after DDS administration. Results Methemoglobin concentrations in the ascorbic acid and MB groups were significantly lower compared to those in the control group across multiple time points. The plasma nitric oxide levels and catalase activity were not different among the groups or time points. Conclusion Intravenous ascorbic acid administration is effective in treating DDS-induced methemoglobinemia in a murine model.http://www.ceemjournal.org/upload/pdf/ceem-17-253.pdfmethemoglobinascorbic acidmethylene blueanti-oxidation |
| spellingShingle | Changwoo Kang Dong Hoon Kim Taeyun Kim Soo Hoon Lee Jin Hee Jeong Sang Bong Lee Jin Hyun Kim Myeong Hee Jung Kyung-woo Lee In Sung Park Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats Clinical and Experimental Emergency Medicine methemoglobin ascorbic acid methylene blue anti-oxidation |
| title | Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats |
| title_full | Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats |
| title_fullStr | Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats |
| title_full_unstemmed | Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats |
| title_short | Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats |
| title_sort | therapeutic effect of ascorbic acid on dapsone induced methemoglobinemia in rats |
| topic | methemoglobin ascorbic acid methylene blue anti-oxidation |
| url | http://www.ceemjournal.org/upload/pdf/ceem-17-253.pdf |
| work_keys_str_mv | AT changwookang therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT donghoonkim therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT taeyunkim therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT soohoonlee therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT jinheejeong therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT sangbonglee therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT jinhyunkim therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT myeongheejung therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT kyungwoolee therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats AT insungpark therapeuticeffectofascorbicacidondapsoneinducedmethemoglobinemiainrats |