Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i>
PCR with primers specific to flanking sequences of gene cassette insertion region and 3'-conservative segment of integrons was used for class 1 integron detection in Burkholderia pseudomallei, B. mallei and B. thailandensis. The sequences of In1 3'-conservative segment were detected in the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2011-04-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/747 |
| _version_ | 1826922482924257280 |
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| author | N. N. Teteryatnikova I. B. Zakharova M. V. Podshivalova A. V. Romanova Ya. A. Lopasteiskaya D. V. Viktorov V. V. Alekseev |
| author_facet | N. N. Teteryatnikova I. B. Zakharova M. V. Podshivalova A. V. Romanova Ya. A. Lopasteiskaya D. V. Viktorov V. V. Alekseev |
| author_sort | N. N. Teteryatnikova |
| collection | DOAJ |
| description | PCR with primers specific to flanking sequences of gene cassette insertion region and 3'-conservative segment of integrons was used for class 1 integron detection in Burkholderia pseudomallei, B. mallei and B. thailandensis. The sequences of In1 3'-conservative segment were detected in the majority of investigated strains of B. pseudomallei. The length of integron cassette insertion region in B. pseudomallei was estimated as 2300 bp (1 strain), 500 and 900 bp (1 strain) and 120 bp (2 strains). The sequence analysis of 3'-segment amlicons showed the presence of qacEdelta1 and sul1 - the determinants of resistance to quaternary ammonium compounds and sulfonamides, respectively. |
| first_indexed | 2024-03-08T13:44:05Z |
| format | Article |
| id | doaj.art-116c709abda14e30b4bd6e51112e077b |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:10:27Z |
| publishDate | 2011-04-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-116c709abda14e30b4bd6e51112e077b2024-12-24T12:09:48ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2011-04-0102(108)464810.21055/0370-1069-2011-2(108)-46-48731Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i>N. N. Teteryatnikova0I. B. Zakharova1M. V. Podshivalova2A. V. Romanova3Ya. A. Lopasteiskaya4D. V. Viktorov5V. V. Alekseev6Volgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstitutePCR with primers specific to flanking sequences of gene cassette insertion region and 3'-conservative segment of integrons was used for class 1 integron detection in Burkholderia pseudomallei, B. mallei and B. thailandensis. The sequences of In1 3'-conservative segment were detected in the majority of investigated strains of B. pseudomallei. The length of integron cassette insertion region in B. pseudomallei was estimated as 2300 bp (1 strain), 500 and 900 bp (1 strain) and 120 bp (2 strains). The sequence analysis of 3'-segment amlicons showed the presence of qacEdelta1 and sul1 - the determinants of resistance to quaternary ammonium compounds and sulfonamides, respectively.https://journal.microbe.ru/jour/article/view/747<i>burkholderia pseudomallei</i>integronsantimicrobial resistance |
| spellingShingle | N. N. Teteryatnikova I. B. Zakharova M. V. Podshivalova A. V. Romanova Ya. A. Lopasteiskaya D. V. Viktorov V. V. Alekseev Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> Проблемы особо опасных инфекций <i>burkholderia pseudomallei</i> integrons antimicrobial resistance |
| title | Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> |
| title_full | Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> |
| title_fullStr | Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> |
| title_full_unstemmed | Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> |
| title_short | Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i> |
| title_sort | molecular detection of class 1 integrons in i burkholderia pseudomallei i |
| topic | <i>burkholderia pseudomallei</i> integrons antimicrobial resistance |
| url | https://journal.microbe.ru/jour/article/view/747 |
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