Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture
Abstract This study investigated the effects of supplementation of low-temperature probiotics isolated from the intestines of olive flounder on the growth performance, digestibility, and regulation of intestinal microbiota and the expression of genes related to growth, immunity, and apoptosis in oli...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52491-9 |
| _version_ | 1827329127091273728 |
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| author | Su-Jeong Lee Da-In Noh Young-Sun Lee Md Tawheed Hasan Sang Woo Hur Seunghan Lee Seong-Mok Jeong Jong Min Lee Eun-Woo Lee Kang-Woong Kim Won Je Jang |
| author_facet | Su-Jeong Lee Da-In Noh Young-Sun Lee Md Tawheed Hasan Sang Woo Hur Seunghan Lee Seong-Mok Jeong Jong Min Lee Eun-Woo Lee Kang-Woong Kim Won Je Jang |
| author_sort | Su-Jeong Lee |
| collection | DOAJ |
| description | Abstract This study investigated the effects of supplementation of low-temperature probiotics isolated from the intestines of olive flounder on the growth performance, digestibility, and regulation of intestinal microbiota and the expression of genes related to growth, immunity, and apoptosis in olive flounder. Bacteria showing high growth at approximately 15–20 °C, which is the temperature of olive flounder culture, were isolated and confirmed to be Pseudomonas species through 16S rRNA gene sequence analysis. Whole-genome sequencing revealed that the strain has a 6,195,122 bp single circular chromosome and a guanine–cytosine content of 59.9%. In the feeding trial, supplementation with 1 × 108 CFU/g of the isolate strain positively modulated growth performances, digestive enzyme activity, and gut microbiota composition of olive flounder. RT-qPCR for the comparison of growth, immunity, and apoptosis-related gene expression levels showed no significant differences between the groups. Therefore, the isolated host-associated low-temperature probiotics improved the growth performance of olive flounder by causing positive changes in digestive activity and intestinal microbial composition without affecting host gene expression. |
| first_indexed | 2024-03-07T15:29:22Z |
| format | Article |
| id | doaj.art-116cd36b26404511883e90476e415ae9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:29:22Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-116cd36b26404511883e90476e415ae92024-03-05T16:29:55ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-024-52491-9Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquacultureSu-Jeong Lee0Da-In Noh1Young-Sun Lee2Md Tawheed Hasan3Sang Woo Hur4Seunghan Lee5Seong-Mok Jeong6Jong Min Lee7Eun-Woo Lee8Kang-Woong Kim9Won Je Jang10Biopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui UniversityBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui UniversityBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui UniversityDepartment of Aquaculture, Sylhet Agricultural UniversityAquafeed Research Center, National Institute of Fisheries ScienceAquafeed Research Center, National Institute of Fisheries ScienceAquafeed Research Center, National Institute of Fisheries ScienceDepartment of Biotechnology, Pukyong National UniversityBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui UniversityAquafeed Research Center, National Institute of Fisheries ScienceBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui UniversityAbstract This study investigated the effects of supplementation of low-temperature probiotics isolated from the intestines of olive flounder on the growth performance, digestibility, and regulation of intestinal microbiota and the expression of genes related to growth, immunity, and apoptosis in olive flounder. Bacteria showing high growth at approximately 15–20 °C, which is the temperature of olive flounder culture, were isolated and confirmed to be Pseudomonas species through 16S rRNA gene sequence analysis. Whole-genome sequencing revealed that the strain has a 6,195,122 bp single circular chromosome and a guanine–cytosine content of 59.9%. In the feeding trial, supplementation with 1 × 108 CFU/g of the isolate strain positively modulated growth performances, digestive enzyme activity, and gut microbiota composition of olive flounder. RT-qPCR for the comparison of growth, immunity, and apoptosis-related gene expression levels showed no significant differences between the groups. Therefore, the isolated host-associated low-temperature probiotics improved the growth performance of olive flounder by causing positive changes in digestive activity and intestinal microbial composition without affecting host gene expression.https://doi.org/10.1038/s41598-024-52491-9 |
| spellingShingle | Su-Jeong Lee Da-In Noh Young-Sun Lee Md Tawheed Hasan Sang Woo Hur Seunghan Lee Seong-Mok Jeong Jong Min Lee Eun-Woo Lee Kang-Woong Kim Won Je Jang Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture Scientific Reports |
| title | Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture |
| title_full | Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture |
| title_fullStr | Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture |
| title_full_unstemmed | Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture |
| title_short | Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture |
| title_sort | effects of host associated low temperature probiotics in olive flounder paralichthys olivaceus aquaculture |
| url | https://doi.org/10.1038/s41598-024-52491-9 |
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