Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo
Both of the surface topographical features and distribution of biochemical cues can influence the cell-substrate interactions and thereby tissue regeneration in vivo. However, they have not been combined simultaneously onto a biodegradable scaffold to demonstrate the synergistic role so far. In this...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21003431 |
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| author | Deteng Zhang Ziming Li Haifei Shi Yuejun Yao Wang Du Pan Lu Kejiong Liang Liangjie Hong Changyou Gao |
| author_facet | Deteng Zhang Ziming Li Haifei Shi Yuejun Yao Wang Du Pan Lu Kejiong Liang Liangjie Hong Changyou Gao |
| author_sort | Deteng Zhang |
| collection | DOAJ |
| description | Both of the surface topographical features and distribution of biochemical cues can influence the cell-substrate interactions and thereby tissue regeneration in vivo. However, they have not been combined simultaneously onto a biodegradable scaffold to demonstrate the synergistic role so far. In this study, a proof-of-concept study is performed to prepare micropatterns and peptide gradient on the inner wall of a poly (D,L-lactide-co-caprolactone) (PLCL) guidance conduit and its advantages in regeneration of peripheral nerve in vivo. After linear ridges/grooves of 20/40 μm in width are created on the PLCL film, its surface is aminolyzed in a kinetically controlled manner to obtain the continuous gradient of amino groups, which are then transferred to CQAASIKVAV peptide density gradient via covalent coupling of glutaraldehyde. The Schwann cells are better aligned along with the stripes, and show a faster migration rate toward the region of higher peptide density. Implantation of the nerve guidance conduit made of the PLCL film having both the micropatterns and peptide gradient can significantly accelerate the regeneration of sciatic nerve in terms of rate, function recovery and microstructures, and reduction of fibrosis in muscle tissues. Moreover, this nerve conduit can also benefit the M2 polarization of macrophages and promote vascularization in vivo. |
| first_indexed | 2024-04-24T08:26:34Z |
| format | Article |
| id | doaj.art-1172448932e6486884cadc0590ba77b4 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:26:34Z |
| publishDate | 2022-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-1172448932e6486884cadc0590ba77b42024-04-16T21:45:56ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-03-019134146Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivoDeteng Zhang0Ziming Li1Haifei Shi2Yuejun Yao3Wang Du4Pan Lu5Kejiong Liang6Liangjie Hong7Changyou Gao8MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaDepartment of Hand Surgery, First Affiliated Hospital of Zhejiang University, School of Medicine. Hangzhou, 310009, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaDepartment of Hand Surgery, First Affiliated Hospital of Zhejiang University, School of Medicine. Hangzhou, 310009, ChinaDepartment of Hand Surgery, First Affiliated Hospital of Zhejiang University, School of Medicine. Hangzhou, 310009, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, China; Corresponding author. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.Both of the surface topographical features and distribution of biochemical cues can influence the cell-substrate interactions and thereby tissue regeneration in vivo. However, they have not been combined simultaneously onto a biodegradable scaffold to demonstrate the synergistic role so far. In this study, a proof-of-concept study is performed to prepare micropatterns and peptide gradient on the inner wall of a poly (D,L-lactide-co-caprolactone) (PLCL) guidance conduit and its advantages in regeneration of peripheral nerve in vivo. After linear ridges/grooves of 20/40 μm in width are created on the PLCL film, its surface is aminolyzed in a kinetically controlled manner to obtain the continuous gradient of amino groups, which are then transferred to CQAASIKVAV peptide density gradient via covalent coupling of glutaraldehyde. The Schwann cells are better aligned along with the stripes, and show a faster migration rate toward the region of higher peptide density. Implantation of the nerve guidance conduit made of the PLCL film having both the micropatterns and peptide gradient can significantly accelerate the regeneration of sciatic nerve in terms of rate, function recovery and microstructures, and reduction of fibrosis in muscle tissues. Moreover, this nerve conduit can also benefit the M2 polarization of macrophages and promote vascularization in vivo.http://www.sciencedirect.com/science/article/pii/S2452199X21003431Peptides gradientMicropatternsContact guidance effectNerve guidance conduitsNerve regeneration |
| spellingShingle | Deteng Zhang Ziming Li Haifei Shi Yuejun Yao Wang Du Pan Lu Kejiong Liang Liangjie Hong Changyou Gao Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo Bioactive Materials Peptides gradient Micropatterns Contact guidance effect Nerve guidance conduits Nerve regeneration |
| title | Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| title_full | Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| title_fullStr | Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| title_full_unstemmed | Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| title_short | Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| title_sort | micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo |
| topic | Peptides gradient Micropatterns Contact guidance effect Nerve guidance conduits Nerve regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21003431 |
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