Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine
Artemisia dracunculus: L. (A. dracunculus) is a popular vegetable and spice cultivated across many Middle Eastern countries. The herb’s aqueous extract has significant folkloric medicinal importance for treating various disorders. Hence, the present investigation aimed to investigate A. dracunculus...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1351743/full |
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| author | Nidal Jaradat Majdi Dwikat Johnny Amer Mustafa Ghanim Mohammed Hawash Fatima Hussein Linda Issa Salsabeel Ishtawe Shahd Salah Sara Nasser |
| author_facet | Nidal Jaradat Majdi Dwikat Johnny Amer Mustafa Ghanim Mohammed Hawash Fatima Hussein Linda Issa Salsabeel Ishtawe Shahd Salah Sara Nasser |
| author_sort | Nidal Jaradat |
| collection | DOAJ |
| description | Artemisia dracunculus: L. (A. dracunculus) is a popular vegetable and spice cultivated across many Middle Eastern countries. The herb’s aqueous extract has significant folkloric medicinal importance for treating various disorders. Hence, the present investigation aimed to investigate A. dracunculus hydrophilic extract phytochemical constituents and pleiotropic biological potentials, as no previous studies have investigated the antilipase and anti-α-amylase effects of the A. dracunculus plant. Total phenol content and phytochemical screening assays were performed utilizing standard analytical methods. While the α-amylase inhibition, free radical-scavenging, antilipase, and cytotoxic activities were determined using dinitrosalicylic acid (DNSA), DPPH, p-nitrophenyl butyrate (PNPB), and MTS assays, respectively. The standard phytochemical analysis of A. dracunculus aqueous extract shows that this extract contains only a phenolic group. The total phenol content was 0.146 ± 0.012 mg GAE/g of the plant dry extract. The A. dracunculus aqueous extract exhibited potent DPPH free radical inhibitory (IC50 dose of 10.71 ± 0.01 μg/mL) and anti-lipase activities (IC50 dose of 60.25 ± 0.33 μg/mL) compared with Trolox (IC50 = 5.7 ± 0.92 μg/mL) and Orlistat (IC50 = 12.3 ± 0.35 μg/mL), respectively. However, it showed a weak anti-α-amylase effect (IC50 value > 1,000 μg/mL) compared with Acarbose (IC50 = 28.18 ± 1.27 μg/mL). A. dracunculus has a cytotoxic effect against the HeLa cancer cell line compared with the chemotherapeutic agent Doxorubicin. The extract has the same percent of inhibition as Doxorubicin (99.9%) at 10 mg/mL. Overall, these results pointed out for the first time the importance of considering A. dracunculus effects as a favorite candidate for preventing and treating metabolic disorders. Also, our results confirm the findings of previous reports on the role of A. dracunculus in the management of cancer and disorders resulting from the accumulation of harmful free radicals. On the contrary, the current study concluded that the antidiabetic role of A. dracunculus could be minimal. Further in-depth investigations are urgently warranted to explore the importance of A. dracunculus in pharmaceutical production. |
| first_indexed | 2024-04-25T02:14:36Z |
| format | Article |
| id | doaj.art-1172ed8777724a51982860acbd4aaed6 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-25T02:14:36Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-1172ed8777724a51982860acbd4aaed62024-03-07T10:14:35ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-03-011510.3389/fphar.2024.13517431351743Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from PalestineNidal Jaradat0Majdi Dwikat1Johnny Amer2Mustafa Ghanim3Mohammed Hawash4Fatima Hussein5Linda Issa6Salsabeel Ishtawe7Shahd Salah8Sara Nasser9Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Allied Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Allied Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, PalestineArtemisia dracunculus: L. (A. dracunculus) is a popular vegetable and spice cultivated across many Middle Eastern countries. The herb’s aqueous extract has significant folkloric medicinal importance for treating various disorders. Hence, the present investigation aimed to investigate A. dracunculus hydrophilic extract phytochemical constituents and pleiotropic biological potentials, as no previous studies have investigated the antilipase and anti-α-amylase effects of the A. dracunculus plant. Total phenol content and phytochemical screening assays were performed utilizing standard analytical methods. While the α-amylase inhibition, free radical-scavenging, antilipase, and cytotoxic activities were determined using dinitrosalicylic acid (DNSA), DPPH, p-nitrophenyl butyrate (PNPB), and MTS assays, respectively. The standard phytochemical analysis of A. dracunculus aqueous extract shows that this extract contains only a phenolic group. The total phenol content was 0.146 ± 0.012 mg GAE/g of the plant dry extract. The A. dracunculus aqueous extract exhibited potent DPPH free radical inhibitory (IC50 dose of 10.71 ± 0.01 μg/mL) and anti-lipase activities (IC50 dose of 60.25 ± 0.33 μg/mL) compared with Trolox (IC50 = 5.7 ± 0.92 μg/mL) and Orlistat (IC50 = 12.3 ± 0.35 μg/mL), respectively. However, it showed a weak anti-α-amylase effect (IC50 value > 1,000 μg/mL) compared with Acarbose (IC50 = 28.18 ± 1.27 μg/mL). A. dracunculus has a cytotoxic effect against the HeLa cancer cell line compared with the chemotherapeutic agent Doxorubicin. The extract has the same percent of inhibition as Doxorubicin (99.9%) at 10 mg/mL. Overall, these results pointed out for the first time the importance of considering A. dracunculus effects as a favorite candidate for preventing and treating metabolic disorders. Also, our results confirm the findings of previous reports on the role of A. dracunculus in the management of cancer and disorders resulting from the accumulation of harmful free radicals. On the contrary, the current study concluded that the antidiabetic role of A. dracunculus could be minimal. Further in-depth investigations are urgently warranted to explore the importance of A. dracunculus in pharmaceutical production.https://www.frontiersin.org/articles/10.3389/fphar.2024.1351743/fullArtemisia dracunculustotal phenolsDPPH free radical inhibitoryantidiabeticantiobesitycytotoxicicity |
| spellingShingle | Nidal Jaradat Majdi Dwikat Johnny Amer Mustafa Ghanim Mohammed Hawash Fatima Hussein Linda Issa Salsabeel Ishtawe Shahd Salah Sara Nasser Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine Frontiers in Pharmacology Artemisia dracunculus total phenols DPPH free radical inhibitory antidiabetic antiobesity cytotoxicicity |
| title | Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine |
| title_full | Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine |
| title_fullStr | Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine |
| title_full_unstemmed | Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine |
| title_short | Total phenolic contents, cytotoxic, free radicals, porcine pancreatic α-amylase, and lipase suppressant activities of Artemisia dracunculus plant from Palestine |
| title_sort | total phenolic contents cytotoxic free radicals porcine pancreatic α amylase and lipase suppressant activities of artemisia dracunculus plant from palestine |
| topic | Artemisia dracunculus total phenols DPPH free radical inhibitory antidiabetic antiobesity cytotoxicicity |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1351743/full |
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