Functional changes of dendritic cells in hypersensivity reactions to amoxicillin
A better understanding of dendritic cell (DC) involvement in responses to haptenic drugs is needed, because it represents a possible approach to the development of an in vitro test, which could identify patients prone to drug allergies. There are two main DC subsets: plasmacytoid DC (pDC) and myeloi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2010-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000007 |
| _version_ | 1828827691923537920 |
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| author | C.M.F. Lima J.T. Schroeder C.E.S. Galvão F.M. Castro J. Kalil N.F. Adkinson Jr. |
| author_facet | C.M.F. Lima J.T. Schroeder C.E.S. Galvão F.M. Castro J. Kalil N.F. Adkinson Jr. |
| author_sort | C.M.F. Lima |
| collection | DOAJ |
| description | A better understanding of dendritic cell (DC) involvement in responses to haptenic drugs is needed, because it represents a possible approach to the development of an in vitro test, which could identify patients prone to drug allergies. There are two main DC subsets: plasmacytoid DC (pDC) and myeloid DC (mDC). β-lactams form hapten-carrier conjugates and may provide a suitable model to study DC behavior in drug allergy reactions. It has been demonstrated that drugs interact differently with DC in drug allergic and non-allergic patients, but there are no studies regarding these subsets. Our aim was to assess the functional changes of mDC and pDC harvested from an amoxicillin-hypersensitive 32-year-old woman who experienced a severe maculopapular exanthema as reflected in interleukin-6 (IL-6) production after stimulation with this drug and penicillin. We also aim to demonstrate, for the first time, the feasibility of this method for dendritic cell isolation followed by in vitro stimulation for studies of drug allergy physiopathology. DC were harvested using a double Percoll density gradient, which generates a basophil-depleted cell (BDC) suspension. Further, pDC were isolated by blood DC antigen 4-positive magnetic selection and gravity filtration through magnetized columns. After stimulation with amoxicillin, penicillin and positive and negative controls, IL-6 production was measured by ELISA. A positive dose-response curve for IL-6 after stimulation with amoxicillin and penicillin was observed for pDC, but not for mDC or BDC suspension. These preliminary results demonstrate the feasibility of this methodology to expand the knowledge of the effect of dendritic cell activation by drug allergens. |
| first_indexed | 2024-12-12T15:04:42Z |
| format | Article |
| id | doaj.art-11749bf2f33f4ad8938dcafcb1fdc30f |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-12T15:04:42Z |
| publishDate | 2010-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-11749bf2f33f4ad8938dcafcb1fdc30f2022-12-22T00:20:44ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2010-10-014310964968Functional changes of dendritic cells in hypersensivity reactions to amoxicillinC.M.F. LimaJ.T. SchroederC.E.S. GalvãoF.M. CastroJ. KalilN.F. Adkinson Jr.A better understanding of dendritic cell (DC) involvement in responses to haptenic drugs is needed, because it represents a possible approach to the development of an in vitro test, which could identify patients prone to drug allergies. There are two main DC subsets: plasmacytoid DC (pDC) and myeloid DC (mDC). β-lactams form hapten-carrier conjugates and may provide a suitable model to study DC behavior in drug allergy reactions. It has been demonstrated that drugs interact differently with DC in drug allergic and non-allergic patients, but there are no studies regarding these subsets. Our aim was to assess the functional changes of mDC and pDC harvested from an amoxicillin-hypersensitive 32-year-old woman who experienced a severe maculopapular exanthema as reflected in interleukin-6 (IL-6) production after stimulation with this drug and penicillin. We also aim to demonstrate, for the first time, the feasibility of this method for dendritic cell isolation followed by in vitro stimulation for studies of drug allergy physiopathology. DC were harvested using a double Percoll density gradient, which generates a basophil-depleted cell (BDC) suspension. Further, pDC were isolated by blood DC antigen 4-positive magnetic selection and gravity filtration through magnetized columns. After stimulation with amoxicillin, penicillin and positive and negative controls, IL-6 production was measured by ELISA. A positive dose-response curve for IL-6 after stimulation with amoxicillin and penicillin was observed for pDC, but not for mDC or BDC suspension. These preliminary results demonstrate the feasibility of this methodology to expand the knowledge of the effect of dendritic cell activation by drug allergens.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000007Dendritic cellsβ-lactam hypersensivityPlasmacytoid dendritic cellsDrug allergy |
| spellingShingle | C.M.F. Lima J.T. Schroeder C.E.S. Galvão F.M. Castro J. Kalil N.F. Adkinson Jr. Functional changes of dendritic cells in hypersensivity reactions to amoxicillin Brazilian Journal of Medical and Biological Research Dendritic cells β-lactam hypersensivity Plasmacytoid dendritic cells Drug allergy |
| title | Functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| title_full | Functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| title_fullStr | Functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| title_full_unstemmed | Functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| title_short | Functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| title_sort | functional changes of dendritic cells in hypersensivity reactions to amoxicillin |
| topic | Dendritic cells β-lactam hypersensivity Plasmacytoid dendritic cells Drug allergy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000007 |
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