Immobility, inheritance and plasticity of shape of the yeast nucleus

<p>Abstract</p> <p>Background</p> <p>Since <it>S. cerevisiae </it>undergoes closed mitosis, the nuclear envelope of the daughter nucleus is continuous with that of the maternal nucleus at anaphase. Nevertheless, several constitutents of the maternal nucleus...

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Main Authors: Andrulis Erik D, Hattier Thomas, Tartakoff Alan M
Format: Article
Language:English
Published: BMC 2007-11-01
Series:BMC Cell Biology
Online Access:http://www.biomedcentral.com/1471-2121/8/47
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author Andrulis Erik D
Hattier Thomas
Tartakoff Alan M
author_facet Andrulis Erik D
Hattier Thomas
Tartakoff Alan M
author_sort Andrulis Erik D
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>Since <it>S. cerevisiae </it>undergoes closed mitosis, the nuclear envelope of the daughter nucleus is continuous with that of the maternal nucleus at anaphase. Nevertheless, several constitutents of the maternal nucleus are not present in the daughter nucleus. The present study aims to identify proteins which impact the shape of the yeast nucleus and to learn whether modifications of shape are passed on to the next mitotic generation. The Esc1p protein of <it>S. cerevisiae </it>localizes to the periphery of the nucleoplasm, can anchor chromatin, and has been implicated in targeted silencing both at telomeres and at HMR.</p> <p>Results</p> <p>Upon increased Esc1p expression, cell division continues and dramatic elaborations of the nuclear envelope extend into the cytoplasm. These "escapades" include nuclear pores and associate with the nucleolus, but exclude chromatin. Escapades are not inherited by daughter nuclei. This exclusion reflects their relative immobility, which we document in studies of prezygotes. Moreover, excess Esc1p affects the levels of multiple transcripts, not all of which originate at telomere-proximal loci. Unlike Esc1p and the colocalizing protein, Mlp1p, overexpression of selected proteins of the inner nuclear membrane is toxic.</p> <p>Conclusion</p> <p>Esc1p is the first non-membrane protein of the nuclear periphery which – like proteins of the nuclear lamina of higher eukaryotes – can modify the shape of the yeast nucleus. The elaborations of the nuclear envelope ("escapades") which appear upon induction of excess Esc1p are not inherited during mitotic growth. The lack of inheritance of such components could help sustain cell growth when parental nuclei have acquired potentially deleterious characteristics.</p>
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spelling doaj.art-1176790278e34d47a873b29cae4d154a2022-12-22T02:11:45ZengBMCBMC Cell Biology1471-21212007-11-01814710.1186/1471-2121-8-47Immobility, inheritance and plasticity of shape of the yeast nucleusAndrulis Erik DHattier ThomasTartakoff Alan M<p>Abstract</p> <p>Background</p> <p>Since <it>S. cerevisiae </it>undergoes closed mitosis, the nuclear envelope of the daughter nucleus is continuous with that of the maternal nucleus at anaphase. Nevertheless, several constitutents of the maternal nucleus are not present in the daughter nucleus. The present study aims to identify proteins which impact the shape of the yeast nucleus and to learn whether modifications of shape are passed on to the next mitotic generation. The Esc1p protein of <it>S. cerevisiae </it>localizes to the periphery of the nucleoplasm, can anchor chromatin, and has been implicated in targeted silencing both at telomeres and at HMR.</p> <p>Results</p> <p>Upon increased Esc1p expression, cell division continues and dramatic elaborations of the nuclear envelope extend into the cytoplasm. These "escapades" include nuclear pores and associate with the nucleolus, but exclude chromatin. Escapades are not inherited by daughter nuclei. This exclusion reflects their relative immobility, which we document in studies of prezygotes. Moreover, excess Esc1p affects the levels of multiple transcripts, not all of which originate at telomere-proximal loci. Unlike Esc1p and the colocalizing protein, Mlp1p, overexpression of selected proteins of the inner nuclear membrane is toxic.</p> <p>Conclusion</p> <p>Esc1p is the first non-membrane protein of the nuclear periphery which – like proteins of the nuclear lamina of higher eukaryotes – can modify the shape of the yeast nucleus. The elaborations of the nuclear envelope ("escapades") which appear upon induction of excess Esc1p are not inherited during mitotic growth. The lack of inheritance of such components could help sustain cell growth when parental nuclei have acquired potentially deleterious characteristics.</p>http://www.biomedcentral.com/1471-2121/8/47
spellingShingle Andrulis Erik D
Hattier Thomas
Tartakoff Alan M
Immobility, inheritance and plasticity of shape of the yeast nucleus
BMC Cell Biology
title Immobility, inheritance and plasticity of shape of the yeast nucleus
title_full Immobility, inheritance and plasticity of shape of the yeast nucleus
title_fullStr Immobility, inheritance and plasticity of shape of the yeast nucleus
title_full_unstemmed Immobility, inheritance and plasticity of shape of the yeast nucleus
title_short Immobility, inheritance and plasticity of shape of the yeast nucleus
title_sort immobility inheritance and plasticity of shape of the yeast nucleus
url http://www.biomedcentral.com/1471-2121/8/47
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