Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01560/full |
| _version_ | 1818821926179569664 |
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| author | Oihane Irazoki Jesus Aranda Timo Zimmermann Susana Campoy Jordi Barbé |
| author_facet | Oihane Irazoki Jesus Aranda Timo Zimmermann Susana Campoy Jordi Barbé |
| author_sort | Oihane Irazoki |
| collection | DOAJ |
| description | In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed. |
| first_indexed | 2024-12-18T23:15:57Z |
| format | Article |
| id | doaj.art-1177bd97940045a29182438ced5aefcf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-18T23:15:57Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1177bd97940045a29182438ced5aefcf2022-12-21T20:48:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-10-01710.3389/fmicb.2016.01560222683Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarmingOihane Irazoki0Jesus Aranda1Timo Zimmermann2Susana Campoy3Jordi Barbé4Universitat Autònoma de BarcelonaUniversitat Autònoma de BarcelonaCenter for Genomic Regulation, BarcelonaUniversitat Autònoma de BarcelonaUniversitat Autònoma de BarcelonaIn addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01560/fullswarmingrecASOS responseChewchemoreceptor polar arrayschemosensory cluster assembly |
| spellingShingle | Oihane Irazoki Jesus Aranda Timo Zimmermann Susana Campoy Jordi Barbé Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming Frontiers in Microbiology swarming recA SOS response Chew chemoreceptor polar arrays chemosensory cluster assembly |
| title | Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming |
| title_full | Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming |
| title_fullStr | Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming |
| title_full_unstemmed | Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming |
| title_short | Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming |
| title_sort | molecular interaction and cellular location of reca and chew proteins in salmonella enterica during sos response and their implication in swarming |
| topic | swarming recA SOS response Chew chemoreceptor polar arrays chemosensory cluster assembly |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01560/full |
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