Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans
Previous studies have found that fecundity is a multigenic trait regulated, in part, by mitochondrial-nuclear (mit-n) genetic interactions. However, the identification of specific nuclear genetic loci or genes interacting with the mitochondrial genome and contributing to the quantitative trait fecun...
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00028/full |
| _version_ | 1811317488069115904 |
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| author | Zuobin Zhu Xiaoxiao Han Yuechen Wang Wei Liu Yue Lu Chang Xu Xitao Wang Lin Hao Yuanjian Song Shi Huang Joshua D. Rizak Ying Li Conghui Han Conghui Han |
| author_facet | Zuobin Zhu Xiaoxiao Han Yuechen Wang Wei Liu Yue Lu Chang Xu Xitao Wang Lin Hao Yuanjian Song Shi Huang Joshua D. Rizak Ying Li Conghui Han Conghui Han |
| author_sort | Zuobin Zhu |
| collection | DOAJ |
| description | Previous studies have found that fecundity is a multigenic trait regulated, in part, by mitochondrial-nuclear (mit-n) genetic interactions. However, the identification of specific nuclear genetic loci or genes interacting with the mitochondrial genome and contributing to the quantitative trait fecundity is an unsolved issue. Here, a panel of recombinant inbred advanced intercrossed lines (RIAILs), established from a cross between the N2 and CB4856 strains of C. elegans, were used to characterize the underlying genetic basis of mit-n genetic interactions related to fecundity. Sixty-seven single nucleotide polymorphisms (SNPs) were identified by association mapping to be linked with fecundity among 115 SNPs linked to mitotype. This indicated significant epistatic effects between nuclear and mitochondria genetics on fecundity. In addition, two specific nuclear genetic loci interacting with the mitochondrial genome and contributing to fecundity were identified. A significant reduction in fecundity was observed in the RIAILs that carried CB4856 mitochondria and a N2 genotype at locus 1 or a CB4856 genotype at locus 2 relative to the wild-type strains. Then, a hybrid strain (CNC10) was established, which was bred as homoplasmic for the CB4856 mtDNA genome and N2 genotype at locus 1 in the CB4856 nuclear background. The mean fecundity of CNC10 was half the fecundity of the control strain. Several functional characteristics of the mitochondria in CNC10 were also influenced by mit-n interactions. Overall, experimental evidence was presented that specific nuclear genetic loci or genes have interactions with the mitochondrial genome and are associated with fecundity. In total, 18 genes were identified using integrative approaches to have interactions with the mitochondrial genome and to contribute to fecundity. |
| first_indexed | 2024-04-13T12:09:00Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-13T12:09:00Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-1178326a323c438a81de3775106592f92022-12-22T02:47:31ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-02-011010.3389/fgene.2019.00028431657Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegansZuobin Zhu0Xiaoxiao Han1Yuechen Wang2Wei Liu3Yue Lu4Chang Xu5Xitao Wang6Lin Hao7Yuanjian Song8Shi Huang9Joshua D. Rizak10Ying Li11Conghui Han12Conghui Han13Department of Genetics, Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, ChinaCenter of Reproductive Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Genetics, Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, ChinaMedical Technology College, Xuzhou Medical University, Xuzhou, ChinaDepartment of Clinical Medicine, Xuzhou Medical University, Xuzhou, ChinaDepartment of Genetics, Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, ChinaDepartment of Urology, Xuzhou Central Hospital, Xuzhou, ChinaDepartment of Urology, Xuzhou Central Hospital, Xuzhou, ChinaDepartment of Genetics, Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, ChinaSchool of Life Sciences, Xiangya Medical School, Central South University, Changsha, ChinaR.A.S. Innovation, Regina, SK, CanadaMedical Technology College, Xuzhou Medical University, Xuzhou, ChinaDepartment of Clinical Medicine, Xuzhou Medical University, Xuzhou, ChinaDepartment of Urology, Xuzhou Central Hospital, Xuzhou, ChinaPrevious studies have found that fecundity is a multigenic trait regulated, in part, by mitochondrial-nuclear (mit-n) genetic interactions. However, the identification of specific nuclear genetic loci or genes interacting with the mitochondrial genome and contributing to the quantitative trait fecundity is an unsolved issue. Here, a panel of recombinant inbred advanced intercrossed lines (RIAILs), established from a cross between the N2 and CB4856 strains of C. elegans, were used to characterize the underlying genetic basis of mit-n genetic interactions related to fecundity. Sixty-seven single nucleotide polymorphisms (SNPs) were identified by association mapping to be linked with fecundity among 115 SNPs linked to mitotype. This indicated significant epistatic effects between nuclear and mitochondria genetics on fecundity. In addition, two specific nuclear genetic loci interacting with the mitochondrial genome and contributing to fecundity were identified. A significant reduction in fecundity was observed in the RIAILs that carried CB4856 mitochondria and a N2 genotype at locus 1 or a CB4856 genotype at locus 2 relative to the wild-type strains. Then, a hybrid strain (CNC10) was established, which was bred as homoplasmic for the CB4856 mtDNA genome and N2 genotype at locus 1 in the CB4856 nuclear background. The mean fecundity of CNC10 was half the fecundity of the control strain. Several functional characteristics of the mitochondria in CNC10 were also influenced by mit-n interactions. Overall, experimental evidence was presented that specific nuclear genetic loci or genes have interactions with the mitochondrial genome and are associated with fecundity. In total, 18 genes were identified using integrative approaches to have interactions with the mitochondrial genome and to contribute to fecundity.https://www.frontiersin.org/article/10.3389/fgene.2019.00028/fullC. elegansfecunditynuclearmitochondriaepistasisgenetics |
| spellingShingle | Zuobin Zhu Xiaoxiao Han Yuechen Wang Wei Liu Yue Lu Chang Xu Xitao Wang Lin Hao Yuanjian Song Shi Huang Joshua D. Rizak Ying Li Conghui Han Conghui Han Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans Frontiers in Genetics C. elegans fecundity nuclear mitochondria epistasis genetics |
| title | Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans |
| title_full | Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans |
| title_fullStr | Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans |
| title_full_unstemmed | Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans |
| title_short | Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans |
| title_sort | identification of specific nuclear genetic loci and genes that interact with the mitochondrial genome and contribute to fecundity in caenorhabditis elegans |
| topic | C. elegans fecundity nuclear mitochondria epistasis genetics |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.00028/full |
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