Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination
Electrochemical DNA sensors are highly demanded for fast and reliable determination of antitumor drugs and chemotherapy monitoring. In this work, an impedimetric DNA sensor has been developed on the base of a phenylamino derivative of phenothiazine (PhTz). A glassy carbon electrode was covered with...
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MDPI AG
2023-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/5/513 |
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| author | Tatjana Kulikova Igor Shiabiev Pavel Padnya Alexey Rogov Gennady Evtugyn Ivan Stoikov Anna Porfireva |
| author_facet | Tatjana Kulikova Igor Shiabiev Pavel Padnya Alexey Rogov Gennady Evtugyn Ivan Stoikov Anna Porfireva |
| author_sort | Tatjana Kulikova |
| collection | DOAJ |
| description | Electrochemical DNA sensors are highly demanded for fast and reliable determination of antitumor drugs and chemotherapy monitoring. In this work, an impedimetric DNA sensor has been developed on the base of a phenylamino derivative of phenothiazine (PhTz). A glassy carbon electrode was covered with electrodeposited product of PhTz oxidation obtained through multiple scans of the potential. The addition of thiacalix[4]arene derivatives bearing four terminal carboxylic groups in the substituents of the lower rim improved the conditions of electropolymerization and affected the performance of the electrochemical sensor depending on the configuration of the macrocyclic core and molar ratio with PhTz molecules in the reaction medium. Following that, the deposition of DNA by physical adsorption was confirmed by atomic force microscopy and electrochemical impedance spectroscopy. The redox properties of the surface layer obtained changed the electron transfer resistance in the presence of doxorubicin due to its intercalating DNA helix and influencing charge distribution on the electrode interface. This made it possible to determine 3 pM–1 nM doxorubicin in 20 min incubation (limit of detection 1.0 pM). The DNA sensor developed was tested on a bovine serum protein solution, Ringer–Locke’s solution mimicking plasma electrolytes and commercial medication (doxorubicin-LANS) and showed a satisfactory recovery rate of 90–105%. The sensor could find applications in pharmacy and medical diagnostics for the assessment of drugs able to specifically bind to DNA. |
| first_indexed | 2024-03-11T03:53:27Z |
| format | Article |
| id | doaj.art-1179d807882a46abb4c74d36e72736f2 |
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| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T03:53:27Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1179d807882a46abb4c74d36e72736f22023-11-18T00:40:45ZengMDPI AGBiosensors2079-63742023-04-0113551310.3390/bios13050513Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin DeterminationTatjana Kulikova0Igor Shiabiev1Pavel Padnya2Alexey Rogov3Gennady Evtugyn4Ivan Stoikov5Anna Porfireva6A.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaA.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaA.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaInterdisciplinary Center of Analytical Microscopy of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaA.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaA.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaA.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, RussiaElectrochemical DNA sensors are highly demanded for fast and reliable determination of antitumor drugs and chemotherapy monitoring. In this work, an impedimetric DNA sensor has been developed on the base of a phenylamino derivative of phenothiazine (PhTz). A glassy carbon electrode was covered with electrodeposited product of PhTz oxidation obtained through multiple scans of the potential. The addition of thiacalix[4]arene derivatives bearing four terminal carboxylic groups in the substituents of the lower rim improved the conditions of electropolymerization and affected the performance of the electrochemical sensor depending on the configuration of the macrocyclic core and molar ratio with PhTz molecules in the reaction medium. Following that, the deposition of DNA by physical adsorption was confirmed by atomic force microscopy and electrochemical impedance spectroscopy. The redox properties of the surface layer obtained changed the electron transfer resistance in the presence of doxorubicin due to its intercalating DNA helix and influencing charge distribution on the electrode interface. This made it possible to determine 3 pM–1 nM doxorubicin in 20 min incubation (limit of detection 1.0 pM). The DNA sensor developed was tested on a bovine serum protein solution, Ringer–Locke’s solution mimicking plasma electrolytes and commercial medication (doxorubicin-LANS) and showed a satisfactory recovery rate of 90–105%. The sensor could find applications in pharmacy and medical diagnostics for the assessment of drugs able to specifically bind to DNA.https://www.mdpi.com/2079-6374/13/5/513electropolymerizationphenothiazine derivativethiacalix[4]arene acidelectrochemical impedance spectroscopyelectrochemical DNA sensordoxorubicin determination |
| spellingShingle | Tatjana Kulikova Igor Shiabiev Pavel Padnya Alexey Rogov Gennady Evtugyn Ivan Stoikov Anna Porfireva Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination Biosensors electropolymerization phenothiazine derivative thiacalix[4]arene acid electrochemical impedance spectroscopy electrochemical DNA sensor doxorubicin determination |
| title | Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination |
| title_full | Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination |
| title_fullStr | Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination |
| title_full_unstemmed | Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination |
| title_short | Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination |
| title_sort | impedimetric dna sensor based on electropolymerized n phenylaminophenothiazine and thiacalix 4 arene tetraacids for doxorubicin determination |
| topic | electropolymerization phenothiazine derivative thiacalix[4]arene acid electrochemical impedance spectroscopy electrochemical DNA sensor doxorubicin determination |
| url | https://www.mdpi.com/2079-6374/13/5/513 |
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