Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm
ABSTRACT Glucose-specific enzyme IIA (EIIAGlc) is a central regulator of bacterial metabolism and an intermediate in the phosphoenolpyruvate phosphotransferase system (PTS), a conserved phosphotransfer cascade that controls carbohydrate transport. We previously reported that EIIAGlc activates transc...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2012-12-01
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| Series: | mBio |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.00228-12 |
| _version_ | 1819038278750306304 |
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| author | Bradley S. Pickering Daniel R. Smith Paula I. Watnick |
| author_facet | Bradley S. Pickering Daniel R. Smith Paula I. Watnick |
| author_sort | Bradley S. Pickering |
| collection | DOAJ |
| description | ABSTRACT Glucose-specific enzyme IIA (EIIAGlc) is a central regulator of bacterial metabolism and an intermediate in the phosphoenolpyruvate phosphotransferase system (PTS), a conserved phosphotransfer cascade that controls carbohydrate transport. We previously reported that EIIAGlc activates transcription of the genes required for Vibrio cholerae biofilm formation. While EIIAGlc modulates the function of many proteins through a direct interaction, none of the known regulatory binding partners of EIIAGlc activates biofilm formation. Therefore, we used tandem affinity purification (TAP) to compare binding partners of EIIAGlc in both planktonic and biofilm cells. A surprising number of novel EIIAGlc binding partners were identified predominantly under one condition or the other. Studies of planktonic cells revealed established partners of EIIAGlc, such as adenylate cyclase and glycerol kinase. In biofilms, MshH, a homolog of Escherichia coli CsrD, was found to be a dominant binding partner of EIIAGlc. Further studies revealed that MshH inhibits biofilm formation. This function was independent of the Carbon storage regulator (Csr) pathway and dependent on EIIAGlc. To explore the existence of multiprotein complexes centered on EIIAGlc, we also affinity purified the binding partners of adenylate cyclase from biofilm cells. In addition to EIIAGlc, this analysis yielded many of the same proteins that copurified with EIIAGlc. We hypothesize that EIIAGlc serves as a hub for multiprotein complexes and furthermore that these complexes may provide a mechanism for competitive and cooperative interactions between binding partners. IMPORTANCE EIIAGlc is a global regulator of microbial physiology that acts through direct interactions with other proteins. This work represents the first demonstration that the protein partners of EIIAGlc are distinct in the microbial biofilm. Furthermore, it provides the first evidence that EIIAGlc may exist in multiprotein complexes with its partners, setting the stage for an investigation of how the multiple partners of EIIAGlc influence one another. Last, it provides a connection between the phosphoenolpyruvate phosphotransferase (PTS) and Csr (Carbon storage regulator) regulatory systems. This work increases our understanding of the complexity of regulation by EIIAGlc and provides a link between the PTS and Csr networks, two global regulatory cascades that influence microbial physiology. |
| first_indexed | 2024-12-21T08:34:46Z |
| format | Article |
| id | doaj.art-117ef15201df45bcbb78e172da75fda7 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-21T08:34:46Z |
| publishDate | 2012-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-117ef15201df45bcbb78e172da75fda72022-12-21T19:10:05ZengAmerican Society for MicrobiologymBio2150-75112012-12-013610.1128/mBio.00228-12Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> BiofilmBradley S. Pickering0Daniel R. Smith1Paula I. Watnick2Division of Infectious Diseases, Boston Children’s Hospital, Boston, Massachusetts, USADivision of Infectious Diseases, Boston Children’s Hospital, Boston, Massachusetts, USADivision of Infectious Diseases, Boston Children’s Hospital, Boston, Massachusetts, USAABSTRACT Glucose-specific enzyme IIA (EIIAGlc) is a central regulator of bacterial metabolism and an intermediate in the phosphoenolpyruvate phosphotransferase system (PTS), a conserved phosphotransfer cascade that controls carbohydrate transport. We previously reported that EIIAGlc activates transcription of the genes required for Vibrio cholerae biofilm formation. While EIIAGlc modulates the function of many proteins through a direct interaction, none of the known regulatory binding partners of EIIAGlc activates biofilm formation. Therefore, we used tandem affinity purification (TAP) to compare binding partners of EIIAGlc in both planktonic and biofilm cells. A surprising number of novel EIIAGlc binding partners were identified predominantly under one condition or the other. Studies of planktonic cells revealed established partners of EIIAGlc, such as adenylate cyclase and glycerol kinase. In biofilms, MshH, a homolog of Escherichia coli CsrD, was found to be a dominant binding partner of EIIAGlc. Further studies revealed that MshH inhibits biofilm formation. This function was independent of the Carbon storage regulator (Csr) pathway and dependent on EIIAGlc. To explore the existence of multiprotein complexes centered on EIIAGlc, we also affinity purified the binding partners of adenylate cyclase from biofilm cells. In addition to EIIAGlc, this analysis yielded many of the same proteins that copurified with EIIAGlc. We hypothesize that EIIAGlc serves as a hub for multiprotein complexes and furthermore that these complexes may provide a mechanism for competitive and cooperative interactions between binding partners. IMPORTANCE EIIAGlc is a global regulator of microbial physiology that acts through direct interactions with other proteins. This work represents the first demonstration that the protein partners of EIIAGlc are distinct in the microbial biofilm. Furthermore, it provides the first evidence that EIIAGlc may exist in multiprotein complexes with its partners, setting the stage for an investigation of how the multiple partners of EIIAGlc influence one another. Last, it provides a connection between the phosphoenolpyruvate phosphotransferase (PTS) and Csr (Carbon storage regulator) regulatory systems. This work increases our understanding of the complexity of regulation by EIIAGlc and provides a link between the PTS and Csr networks, two global regulatory cascades that influence microbial physiology.https://journals.asm.org/doi/10.1128/mBio.00228-12 |
| spellingShingle | Bradley S. Pickering Daniel R. Smith Paula I. Watnick Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm mBio |
| title | Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm |
| title_full | Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm |
| title_fullStr | Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm |
| title_full_unstemmed | Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm |
| title_short | Glucose-Specific Enzyme IIA Has Unique Binding Partners in The <named-content content-type="genus-species">Vibrio cholerae</named-content> Biofilm |
| title_sort | glucose specific enzyme iia has unique binding partners in the named content content type genus species vibrio cholerae named content biofilm |
| url | https://journals.asm.org/doi/10.1128/mBio.00228-12 |
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