Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
Abstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry pa...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83293-y |
| _version_ | 1818842724140318720 |
|---|---|
| author | Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram |
| author_facet | Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram |
| author_sort | Sina Dadgar |
| collection | DOAJ |
| description | Abstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry parameters. Here, we used diffuse reflectance spectroscopy (DRS) as a novel non-invasive method to directly determine changes in hematological parameters in the breast tissues of live heat-stressed broilers. Three-week-old male broilers were randomly subjected to two environmental conditions (thermoneutral, TN, 24 °C vs. cyclic heat stress, HS, 35 °C, 12 h/day). Optical spectra were acquired using DRS to monitor breast hemoglobin (Hb) concentration and vascular oxygen saturation (sO2) at three time points: at baseline prior to heat stress, 2 days, and 21 days after initiation of HS. While i-STAT did not demonstrate a discernible change due to HS in circulatory hemoglobin, DRS found a significant decrease in breast Hb and sO2 after exposure to chronic HS. The decrease in sO2 was found to be due to a decrease in oxygenated hemoglobin concentration, indicating a large increase in oxygen consumption in heat-stressed broilers. Our results demonstrate that DRS could potentially be used to study the effects of HS directly in specific organs of interest, such as the breast and thigh, to improve meat quality. |
| first_indexed | 2024-12-19T04:46:31Z |
| format | Article |
| id | doaj.art-11810ecfb263419190095721d305d790 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:46:31Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-11810ecfb263419190095721d305d7902022-12-21T20:35:27ZengNature PortfolioScientific Reports2045-23222021-02-011111810.1038/s41598-021-83293-yDiffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breastSina Dadgar0Elizabeth Greene1Ahmed Dhamad2Barbara Mallmann3Sami Dridi4Narasimhan Rajaram5Department of Biomedical Engineering, University of ArkansasDepartment of Poultry Science, University of ArkansasDepartment of Poultry Science, University of ArkansasDepartment of Poultry Science, University of ArkansasDepartment of Poultry Science, University of ArkansasDepartment of Biomedical Engineering, University of ArkansasAbstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry parameters. Here, we used diffuse reflectance spectroscopy (DRS) as a novel non-invasive method to directly determine changes in hematological parameters in the breast tissues of live heat-stressed broilers. Three-week-old male broilers were randomly subjected to two environmental conditions (thermoneutral, TN, 24 °C vs. cyclic heat stress, HS, 35 °C, 12 h/day). Optical spectra were acquired using DRS to monitor breast hemoglobin (Hb) concentration and vascular oxygen saturation (sO2) at three time points: at baseline prior to heat stress, 2 days, and 21 days after initiation of HS. While i-STAT did not demonstrate a discernible change due to HS in circulatory hemoglobin, DRS found a significant decrease in breast Hb and sO2 after exposure to chronic HS. The decrease in sO2 was found to be due to a decrease in oxygenated hemoglobin concentration, indicating a large increase in oxygen consumption in heat-stressed broilers. Our results demonstrate that DRS could potentially be used to study the effects of HS directly in specific organs of interest, such as the breast and thigh, to improve meat quality.https://doi.org/10.1038/s41598-021-83293-y |
| spellingShingle | Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast Scientific Reports |
| title | Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast |
| title_full | Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast |
| title_fullStr | Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast |
| title_full_unstemmed | Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast |
| title_short | Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast |
| title_sort | diffuse reflectance spectroscopy reveals heat stress induced changes in hemoglobin concentration in chicken breast |
| url | https://doi.org/10.1038/s41598-021-83293-y |
| work_keys_str_mv | AT sinadadgar diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT elizabethgreene diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT ahmeddhamad diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT barbaramallmann diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT samidridi diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT narasimhanrajaram diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast |