Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography
The link between the structural properties and the rupturing of chordae tendineae in the mitral valve complex is still unclear. Synchrotron-radiation-based X-ray phase computed tomography (SR-XPCT) imaging is an innovative way to quantitatively analyze three-dimensional morphology. XPCT has been emp...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2023-09-01
|
| Series: | Journal of Synchrotron Radiation |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S1600577523005167 |
| _version_ | 1797691473131470848 |
|---|---|
| author | Yojiro Koda Takuro Tsukube Masato Hoshino Naoto Yagi Hatsue Ishibashi-Ueda Kenji Okada |
| author_facet | Yojiro Koda Takuro Tsukube Masato Hoshino Naoto Yagi Hatsue Ishibashi-Ueda Kenji Okada |
| author_sort | Yojiro Koda |
| collection | DOAJ |
| description | The link between the structural properties and the rupturing of chordae tendineae in the mitral valve complex is still unclear. Synchrotron-radiation-based X-ray phase computed tomography (SR-XPCT) imaging is an innovative way to quantitatively analyze three-dimensional morphology. XPCT has been employed in this study to evaluate the chordae tendineae from patients with mitral regurgitation and to analyze structural changes in the ruptured chordae tendineae in patients with this condition. Six ruptured mitral chordae tendineae were obtained during surgical repairs for mitral regurgitation and were fixed with formalin. In addition, 12 healthy chordae tendineae were obtained from autopsies. Employing XPCT (effective pixel size, 3.5 µm; density resolution, 1 mg cm−3), the density of the chordae tendineae in each sample was measured. The specimens were subsequently analyzed pathologically. The mean age was 70.2 ± 3.0 in the rupture group and 67.2 ± 14.1 years old in the control group (p = 0.4927). All scans of chorda tendineae with SR-XPCT were performed successfully. The mean densities were 1.029 ± 0.004 in the rupture group and 1.085 ± 0.015 g cm−3 in the control group (p < 0.0001). Density based on SR-XPCT in the ruptured mitral chordae tendineae was significantly lower compared with the healthy chorda tendinea. Histological examination revealed a change in the components of the connective tissues in ruptured chorda tendinea, in accordance with the low density measured by SR-XPCT. SR-XPCT made it possible to measure tissue density in mitral chordae tendineae. Low density in mitral chordae tendineae is associated with a greater fragility in ruptured mitral chordae tendineae. |
| first_indexed | 2024-03-12T02:14:46Z |
| format | Article |
| id | doaj.art-118420e57779443da454394858388fec |
| institution | Directory Open Access Journal |
| issn | 1600-5775 |
| language | English |
| last_indexed | 2024-03-12T02:14:46Z |
| publishDate | 2023-09-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj.art-118420e57779443da454394858388fec2023-09-06T09:19:05ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752023-09-01305995100210.1107/S1600577523005167yi5137Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomographyYojiro Koda0Takuro Tsukube1Masato Hoshino2Naoto Yagi3Hatsue Ishibashi-Ueda4Kenji Okada5Department of Surgery, Division of Cardiovascular Surgery, Kobe University Hospital, Kobe, JapanDepartment of Surgery, Division of Cardiovascular Surgery, Kobe University Hospital, Kobe, JapanScattering and Imaging Division, Japan Synchrotron Radiation Research Institute, Sayo, JapanScattering and Imaging Division, Japan Synchrotron Radiation Research Institute, Sayo, JapanDepartment of Pathology, Hokusetsu General Hospital, Takatsuki, Osaka, JapanDepartment of Surgery, Division of Cardiovascular Surgery, Kobe University Hospital, Kobe, JapanThe link between the structural properties and the rupturing of chordae tendineae in the mitral valve complex is still unclear. Synchrotron-radiation-based X-ray phase computed tomography (SR-XPCT) imaging is an innovative way to quantitatively analyze three-dimensional morphology. XPCT has been employed in this study to evaluate the chordae tendineae from patients with mitral regurgitation and to analyze structural changes in the ruptured chordae tendineae in patients with this condition. Six ruptured mitral chordae tendineae were obtained during surgical repairs for mitral regurgitation and were fixed with formalin. In addition, 12 healthy chordae tendineae were obtained from autopsies. Employing XPCT (effective pixel size, 3.5 µm; density resolution, 1 mg cm−3), the density of the chordae tendineae in each sample was measured. The specimens were subsequently analyzed pathologically. The mean age was 70.2 ± 3.0 in the rupture group and 67.2 ± 14.1 years old in the control group (p = 0.4927). All scans of chorda tendineae with SR-XPCT were performed successfully. The mean densities were 1.029 ± 0.004 in the rupture group and 1.085 ± 0.015 g cm−3 in the control group (p < 0.0001). Density based on SR-XPCT in the ruptured mitral chordae tendineae was significantly lower compared with the healthy chorda tendinea. Histological examination revealed a change in the components of the connective tissues in ruptured chorda tendinea, in accordance with the low density measured by SR-XPCT. SR-XPCT made it possible to measure tissue density in mitral chordae tendineae. Low density in mitral chordae tendineae is associated with a greater fragility in ruptured mitral chordae tendineae.http://scripts.iucr.org/cgi-bin/paper?S1600577523005167synchrotron-based x-ray phase computed tomographymitral valvemitral chordamitral chorda rupturemitral valve structure |
| spellingShingle | Yojiro Koda Takuro Tsukube Masato Hoshino Naoto Yagi Hatsue Ishibashi-Ueda Kenji Okada Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography Journal of Synchrotron Radiation synchrotron-based x-ray phase computed tomography mitral valve mitral chorda mitral chorda rupture mitral valve structure |
| title | Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography |
| title_full | Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography |
| title_fullStr | Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography |
| title_full_unstemmed | Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography |
| title_short | Structural properties in ruptured mitral chordae tendineae measured by synchrotron-based X-ray phase computed tomography |
| title_sort | structural properties in ruptured mitral chordae tendineae measured by synchrotron based x ray phase computed tomography |
| topic | synchrotron-based x-ray phase computed tomography mitral valve mitral chorda mitral chorda rupture mitral valve structure |
| url | http://scripts.iucr.org/cgi-bin/paper?S1600577523005167 |
| work_keys_str_mv | AT yojirokoda structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography AT takurotsukube structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography AT masatohoshino structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography AT naotoyagi structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography AT hatsueishibashiueda structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography AT kenjiokada structuralpropertiesinrupturedmitralchordaetendineaemeasuredbysynchrotronbasedxrayphasecomputedtomography |