Isolation of mecC Gene carrying Methicillin Resistant Staphylococcus aureus in Clinical Samples from a Tertiary Care Institute, Northern India
Introduction: The MecC Methicillin Resistant Staphylococcus aureus (MRSA) after its initial recovery in 2007 has been reported with varying frequency from different parts of the world. These isolates assume importance due to the fact that with routine testing platforms available for the detectio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2021-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/15525/50312_CE[Ra1]_F[SH]_PF1(AG_SL)_PFA(SS_AG_KM)_PN(KM).pdf |
| Summary: | Introduction: The MecC Methicillin Resistant Staphylococcus
aureus (MRSA) after its initial recovery in 2007 has been reported
with varying frequency from different parts of the world. These
isolates assume importance due to the fact that with routine
testing platforms available for the detection of MRSA they
can be misidentified as being methicillin sensitive which can
adversely affect the treatment and outcome of infections due to
MRSA harbouring the mecC gene.
Aim: To evaluate mecC gene carrying MRSA in clinical isolates.
Materials and Methods: This retrospective study was conducted
in the Department of Microbiology, Sher-i-Kashmir Institute of
Medical Sciences, Srinagar, Jammu and Kashmir, India for a
period of three months (May-July 2020). A total of 102 laboratory
confirmed isolates of MRSA (based on biochemical tests and
cefoxitin disc diffusion results) were subjected to screening for the
presence of mecA and mecC gene by Polymerase Chain Reaction
(PCR). Deoxyribonucleic Acid (DNA) was extracted using an in
house extraction method following which mecA and mecC were
amplified in a total reaction volume of 25 μL using 2x PCR master
mix, 5 μL of template and 1 μL (0.4 μM final concentration) each
of reverse and forward primers specific for the above mentioned
genes. Statistical analysis was done using Statistical Package for
the Social Sciences (SPSS) software v24.0.
Results: All the isolates were confirmed as being methicillin
resistant with 96.1% isolates carrying the mecA gene and 3.9%
carrying the mecC gene. The mecC MRSA were recovered from
pus, swab and endotracheal tip in middle aged men. One of the
patient from whose sample mecC MRSA was recovered was
suffering from hypertension, diabetes and renal faliure. MRSA
exhibited high resistance to all the antimicrobial agents tested
however all of them were sensitive to vancomycin and linezolid.
Conclusion: The presence of mecC gene in clinical isolates of
MRSA is a cause of concern and calls for an extensive and
continuous surveillance of such isolates as they can in future be
implicated in causing severe human infections. |
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| ISSN: | 2249-782X 0973-709X |