Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence
Bacteria of the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> are symbionts of entomopathogenic nematodes. Despite their close phylogenetic relationship, they show differences in their pathogenicity and virulence mechanisms in target insects. These differences were explor...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/486 |
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| author | Abraham Rivera-Ramírez Rosalba Salgado-Morales Alfredo Jiménez-Pérez Rebeca Pérez-Martínez Blanca Inés García-Gómez Edgar Dantán-González |
| author_facet | Abraham Rivera-Ramírez Rosalba Salgado-Morales Alfredo Jiménez-Pérez Rebeca Pérez-Martínez Blanca Inés García-Gómez Edgar Dantán-González |
| author_sort | Abraham Rivera-Ramírez |
| collection | DOAJ |
| description | Bacteria of the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> are symbionts of entomopathogenic nematodes. Despite their close phylogenetic relationship, they show differences in their pathogenicity and virulence mechanisms in target insects. These differences were explored by the analysis of the pangenome, as it provides a framework for characterizing and defining the gene repertoire. We performed the first pangenome analysis of 91 strains of <i>Xenorhabdus</i> and <i>Photorhabdus</i>; the analysis showed that the <i>Photorhabdus</i> genus has a higher number of genes associated with pathogenicity. However, biological tests showed that whole cells of <i>X. nematophila</i> SC 0516 were more virulent than those of <i>P. luminescens</i> HIM3 when both were injected into <i>G. mellonella</i> larvae. In addition, we cloned and expressed the GroEL proteins of both bacteria, as this protein has been previously indicated to show insecticidal activity in the genus <i>Xenorhabdus</i>. Among these proteins, Cpn60-Xn was found to be the most toxic at all concentrations tested, with an LC50 value of 102.34 ng/larva. Sequence analysis suggested that the Cpn60-Xn toxin was homologous to Cpn60-Pl; however, Cpn60-Xn contained thirty-five differentially substituted amino acid residues that could be responsible for its insecticidal activity. |
| first_indexed | 2024-03-09T13:16:08Z |
| format | Article |
| id | doaj.art-118a43e295e249c5800638d480288e82 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:08Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-118a43e295e249c5800638d480288e822023-11-30T21:34:57ZengMDPI AGMicroorganisms2076-26072022-02-0110348610.3390/microorganisms10030486Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in VirulenceAbraham Rivera-Ramírez0Rosalba Salgado-Morales1Alfredo Jiménez-Pérez2Rebeca Pérez-Martínez3Blanca Inés García-Gómez4Edgar Dantán-González5Instituto de Investigaciones en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, MexicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Calle Ceprobi No. 8, San Isidro, Yautepec 62739, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, MexicoInstituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca 62250, Morelos, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, MexicoBacteria of the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> are symbionts of entomopathogenic nematodes. Despite their close phylogenetic relationship, they show differences in their pathogenicity and virulence mechanisms in target insects. These differences were explored by the analysis of the pangenome, as it provides a framework for characterizing and defining the gene repertoire. We performed the first pangenome analysis of 91 strains of <i>Xenorhabdus</i> and <i>Photorhabdus</i>; the analysis showed that the <i>Photorhabdus</i> genus has a higher number of genes associated with pathogenicity. However, biological tests showed that whole cells of <i>X. nematophila</i> SC 0516 were more virulent than those of <i>P. luminescens</i> HIM3 when both were injected into <i>G. mellonella</i> larvae. In addition, we cloned and expressed the GroEL proteins of both bacteria, as this protein has been previously indicated to show insecticidal activity in the genus <i>Xenorhabdus</i>. Among these proteins, Cpn60-Xn was found to be the most toxic at all concentrations tested, with an LC50 value of 102.34 ng/larva. Sequence analysis suggested that the Cpn60-Xn toxin was homologous to Cpn60-Pl; however, Cpn60-Xn contained thirty-five differentially substituted amino acid residues that could be responsible for its insecticidal activity.https://www.mdpi.com/2076-2607/10/3/486<i>Xenorhabdus</i><i>Photorhabdus</i>pangenomepathogenicity<i>G. mellonella</i>chaperonin |
| spellingShingle | Abraham Rivera-Ramírez Rosalba Salgado-Morales Alfredo Jiménez-Pérez Rebeca Pérez-Martínez Blanca Inés García-Gómez Edgar Dantán-González Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence Microorganisms <i>Xenorhabdus</i> <i>Photorhabdus</i> pangenome pathogenicity <i>G. mellonella</i> chaperonin |
| title | Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence |
| title_full | Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence |
| title_fullStr | Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence |
| title_full_unstemmed | Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence |
| title_short | Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence |
| title_sort | comparative genomics and pathogenicity analysis of two bacterial symbionts of entomopathogenic nematodes the role of the groel protein in virulence |
| topic | <i>Xenorhabdus</i> <i>Photorhabdus</i> pangenome pathogenicity <i>G. mellonella</i> chaperonin |
| url | https://www.mdpi.com/2076-2607/10/3/486 |
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