A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes
Freshwater bodies are key in supporting aquatic and terrestrial life. Ecological balance of freshwater habitats is very vulnerable, hence, often significantly disrupted by climatic changes and anthropogenic acts. In Israel, due to its relatively arid climate, many freshwater resources have been disr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2022.1010838/full |
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| author | Roni Tadmor-Levi Roni Tadmor-Levi David Cummings Tomer Borovski Rachel Shapira Evgeniya Marcos-Hadad Lior David Lior David |
| author_facet | Roni Tadmor-Levi Roni Tadmor-Levi David Cummings Tomer Borovski Rachel Shapira Evgeniya Marcos-Hadad Lior David Lior David |
| author_sort | Roni Tadmor-Levi |
| collection | DOAJ |
| description | Freshwater bodies are key in supporting aquatic and terrestrial life. Ecological balance of freshwater habitats is very vulnerable, hence, often significantly disrupted by climatic changes and anthropogenic acts. In Israel, due to its relatively arid climate, many freshwater resources have been disrupted and still are under great pressure. The Sea of Galilee is the largest surface freshwater body in the Middle East and a habitat to unique populations of several fishes, including six cichlid species. Studies on the ecology of these fish and their conservation require effective monitoring tools. In this study, a simple and efficient molecular method was developed to identify the species of these lake cichlids using high resolution melting analysis of mini DNA barcodes. The species of an individual sample can be identified by a single tube PCR reaction. This assay successfully identified sequence differences both among and within species. Here, this method identified the species for 279 small cichlid fry that could not be morphologically identified, allowing to estimate relative species abundance and map their distribution in time and location. The results are key to understand not only the ecology of young stages but also their recruitment potential to adult fish populations and their sustainability. This method can be readily implemented in further ecological studies and surveys related to these species, in the lake and its surroundings, as a tool to enhance understanding and protection of these species. |
| first_indexed | 2024-04-11T11:28:51Z |
| format | Article |
| id | doaj.art-118d030c7fdd4ab2a82e0222a08453da |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-04-11T11:28:51Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-118d030c7fdd4ab2a82e0222a08453da2022-12-22T04:26:12ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2022-09-011010.3389/fevo.2022.10108381010838A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodesRoni Tadmor-Levi0Roni Tadmor-Levi1David Cummings2Tomer Borovski3Rachel Shapira4Evgeniya Marcos-Hadad5Lior David6Lior David7Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelNational Natural History Collections, The Hebrew University of Jerusalem, Jerusalem, IsraelKinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, IsraelDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelNational Natural History Collections, The Hebrew University of Jerusalem, Jerusalem, IsraelFreshwater bodies are key in supporting aquatic and terrestrial life. Ecological balance of freshwater habitats is very vulnerable, hence, often significantly disrupted by climatic changes and anthropogenic acts. In Israel, due to its relatively arid climate, many freshwater resources have been disrupted and still are under great pressure. The Sea of Galilee is the largest surface freshwater body in the Middle East and a habitat to unique populations of several fishes, including six cichlid species. Studies on the ecology of these fish and their conservation require effective monitoring tools. In this study, a simple and efficient molecular method was developed to identify the species of these lake cichlids using high resolution melting analysis of mini DNA barcodes. The species of an individual sample can be identified by a single tube PCR reaction. This assay successfully identified sequence differences both among and within species. Here, this method identified the species for 279 small cichlid fry that could not be morphologically identified, allowing to estimate relative species abundance and map their distribution in time and location. The results are key to understand not only the ecology of young stages but also their recruitment potential to adult fish populations and their sustainability. This method can be readily implemented in further ecological studies and surveys related to these species, in the lake and its surroundings, as a tool to enhance understanding and protection of these species.https://www.frontiersin.org/articles/10.3389/fevo.2022.1010838/fullBar-HRMSea of GalileetilapiaCOIDNA barcodingfreshwater fish ecology |
| spellingShingle | Roni Tadmor-Levi Roni Tadmor-Levi David Cummings Tomer Borovski Rachel Shapira Evgeniya Marcos-Hadad Lior David Lior David A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes Frontiers in Ecology and Evolution Bar-HRM Sea of Galilee tilapia COI DNA barcoding freshwater fish ecology |
| title | A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes |
| title_full | A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes |
| title_fullStr | A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes |
| title_full_unstemmed | A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes |
| title_short | A method for quick and efficient identification of cichlid species by high resolution DNA melting analysis of minibarcodes |
| title_sort | method for quick and efficient identification of cichlid species by high resolution dna melting analysis of minibarcodes |
| topic | Bar-HRM Sea of Galilee tilapia COI DNA barcoding freshwater fish ecology |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2022.1010838/full |
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