Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor
The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-ami...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2014-09-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400011&lng=en&tlng=en |
| _version_ | 1819156072354545664 |
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| author | Hui-Liang Li Dong Guo Shi-Qing Peng |
| author_facet | Hui-Liang Li Dong Guo Shi-Qing Peng |
| author_sort | Hui-Liang Li |
| collection | DOAJ |
| description | The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated by polyethylene glycol, NaCl, and cold treatments, as well as by abscisic acid, jasmonic acid, and ethylene treatment. Analysis of transgenic tobacco plants over-expressing JcR1MYB1 indicates an inportant function for this gene in salt stress. |
| first_indexed | 2024-12-22T15:47:03Z |
| format | Article |
| id | doaj.art-118e9582ea0b4e96bbf7f72c2f14048b |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T15:47:03Z |
| publishDate | 2014-09-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-118e9582ea0b4e96bbf7f72c2f14048b2022-12-21T18:21:00ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852014-09-0137354955510.1590/S1415-47572014000400011S1415-47572014000400011Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factorHui-Liang Li0Dong Guo1Shi-Qing Peng2Chinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesChinese Academy of Tropical Agricultural SciencesThe cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated by polyethylene glycol, NaCl, and cold treatments, as well as by abscisic acid, jasmonic acid, and ethylene treatment. Analysis of transgenic tobacco plants over-expressing JcR1MYB1 indicates an inportant function for this gene in salt stress.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400011&lng=en&tlng=enabiotic stressgene expressionJatropha curcasR1-MYB transcription factor |
| spellingShingle | Hui-Liang Li Dong Guo Shi-Qing Peng Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor Genetics and Molecular Biology abiotic stress gene expression Jatropha curcas R1-MYB transcription factor |
| title | Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor |
| title_full | Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor |
| title_fullStr | Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor |
| title_full_unstemmed | Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor |
| title_short | Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor |
| title_sort | molecular characterization of the jatropha curcas jcr1myb1 gene encoding a putative r1 myb transcription factor |
| topic | abiotic stress gene expression Jatropha curcas R1-MYB transcription factor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400011&lng=en&tlng=en |
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