Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex
Carboxylate anions are analytical targets with environmental and biological relevance, whose detection is often challenging in aqueous solutions. We describe a method for discrimination and quantitation of carboxylates in water buffered to pH 7.4 based on their differential interaction with a supram...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/11/3637 |
| _version_ | 1797532906611015680 |
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| author | Yifei Xu Marco Bonizzoni |
| author_facet | Yifei Xu Marco Bonizzoni |
| author_sort | Yifei Xu |
| collection | DOAJ |
| description | Carboxylate anions are analytical targets with environmental and biological relevance, whose detection is often challenging in aqueous solutions. We describe a method for discrimination and quantitation of carboxylates in water buffered to pH 7.4 based on their differential interaction with a supramolecular fluorescent sensor, self-assembled from readily available building blocks. A fifth-generation poly(amidoamine) dendrimer (PAMAM G5), bound to organic fluorophores (calcein or pyranine) through noncovalent interactions, forms a [dye•PAMAM] complex responsive to interaction with carboxylates. The observed changes in absorbance, and in fluorescence emission and anisotropy, were interpreted through linear discriminant analysis (LDA) and principal component analysis (PCA) to differentiate 10 structurally similar carboxylates with a limit of discrimination around 100 μM. The relationship between the analytes’ chemical structures and the system’s response was also elucidated. This insight allowed us to extend the system’s capabilities to the simultaneous identification of the nature and concentration of unknown analytes, with excellent structural identification results and good concentration recovery, an uncommon feat for a pattern-based sensing system. |
| first_indexed | 2024-03-10T11:07:03Z |
| format | Article |
| id | doaj.art-1191c921fbc54d11b92bd746eb42da50 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T11:07:03Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1191c921fbc54d11b92bd746eb42da502023-11-21T21:04:05ZengMDPI AGSensors1424-82202021-05-012111363710.3390/s21113637Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] ComplexYifei Xu0Marco Bonizzoni1Department of Chemistry and Biochemistry, The University of Alabama, Tuscaloosa, AL 35487, USADepartment of Chemistry and Biochemistry, The University of Alabama, Tuscaloosa, AL 35487, USACarboxylate anions are analytical targets with environmental and biological relevance, whose detection is often challenging in aqueous solutions. We describe a method for discrimination and quantitation of carboxylates in water buffered to pH 7.4 based on their differential interaction with a supramolecular fluorescent sensor, self-assembled from readily available building blocks. A fifth-generation poly(amidoamine) dendrimer (PAMAM G5), bound to organic fluorophores (calcein or pyranine) through noncovalent interactions, forms a [dye•PAMAM] complex responsive to interaction with carboxylates. The observed changes in absorbance, and in fluorescence emission and anisotropy, were interpreted through linear discriminant analysis (LDA) and principal component analysis (PCA) to differentiate 10 structurally similar carboxylates with a limit of discrimination around 100 μM. The relationship between the analytes’ chemical structures and the system’s response was also elucidated. This insight allowed us to extend the system’s capabilities to the simultaneous identification of the nature and concentration of unknown analytes, with excellent structural identification results and good concentration recovery, an uncommon feat for a pattern-based sensing system.https://www.mdpi.com/1424-8220/21/11/3637carboxylatesensingpatternfluorescencedendrimerquantitation |
| spellingShingle | Yifei Xu Marco Bonizzoni Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex Sensors carboxylate sensing pattern fluorescence dendrimer quantitation |
| title | Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex |
| title_full | Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex |
| title_fullStr | Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex |
| title_full_unstemmed | Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex |
| title_short | Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex |
| title_sort | discrimination and quantitation of biologically relevant carboxylate anions using a dye•pamam complex |
| topic | carboxylate sensing pattern fluorescence dendrimer quantitation |
| url | https://www.mdpi.com/1424-8220/21/11/3637 |
| work_keys_str_mv | AT yifeixu discriminationandquantitationofbiologicallyrelevantcarboxylateanionsusingadyepamamcomplex AT marcobonizzoni discriminationandquantitationofbiologicallyrelevantcarboxylateanionsusingadyepamamcomplex |