Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress
The protective effects against drought stress of the endophytic bacterium <i>Bacillus subtilis</i> 10-4 were measured by studying the priming response in two wheat (<i>Triticum aestivum</i> L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought,...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1810 |
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| author | Oksana Lastochkina Darya Garshina Sergey Ivanov Ruslan Yuldashev Regina Khafizova Chulpan Allagulova Kristina Fedorova Azamat Avalbaev Dilara Maslennikova Massimo Bosacchi |
| author_facet | Oksana Lastochkina Darya Garshina Sergey Ivanov Ruslan Yuldashev Regina Khafizova Chulpan Allagulova Kristina Fedorova Azamat Avalbaev Dilara Maslennikova Massimo Bosacchi |
| author_sort | Oksana Lastochkina |
| collection | DOAJ |
| description | The protective effects against drought stress of the endophytic bacterium <i>Bacillus subtilis</i> 10-4 were measured by studying the priming response in two wheat (<i>Triticum aestivum</i> L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought, respectively. <i>B. subtilis</i> 10-4 improved germination and growth parameters under normal conditions in both cultivars with the most pronounced effect observed in cv. E70. Under drought conditions, <i>B. subtilis</i> 10-4 significantly ameliorated the negative impact of stress on germination and growth of cv. E70, but had no protective effect on cv. SY. <i>B. subtilis</i> 10-4 induced an increase in the levels of photosynthetic chlorophyll (Chl) a, Chl b, and carotenoids (Car) in the leaves of cv. E70, both under normal and drought conditions. In cv. SY plants, bacterial inoculation decreased the contents of Chl a, Chl b, and Car under normal conditions, but pigment content were almost recovered under drought stress. <i>B. subtilis</i> 10-4 increased water holding capacity (WHC) of cv. E70 (but did not affect this parameter in cv. SY) and prevented the stress-induced decline in WHC in both cultivars. Notably, <i>B. subtilis</i> 10-4 increased endogenous salicylic acid (SA) concentration in both cultivars, especially in cv. E70. Moreover, <i>B. subtilis</i> 10-4 reduced drought-induced endogenous SA accumulation, which was correlated with the influence of endophyte on growth, indicating a possible involvement of endogenous SA in the implementation of <i>B. subtilis</i>-mediated effects in both cultivars. Overall, <i>B. subtilis</i> 10-4 inoculation was found to increase drought tolerance in seedlings of both cultivars, as evidenced by decreased lipid peroxidation, proline content, and electrolyte leakage from tissues of wheat seedlings primed with <i>B. subtilis</i> 10-4 under drought conditions. |
| first_indexed | 2024-03-10T13:53:16Z |
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| spelling | doaj.art-1191ca7df3f94f8fab0ad4b8326dc2402023-11-21T01:51:22ZengMDPI AGPlants2223-77472020-12-01912181010.3390/plants9121810Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought StressOksana Lastochkina0Darya Garshina1Sergey Ivanov2Ruslan Yuldashev3Regina Khafizova4Chulpan Allagulova5Kristina Fedorova6Azamat Avalbaev7Dilara Maslennikova8Massimo Bosacchi9Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaBashkir Research Institute of Agriculture—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450059, RussiaUfa Institute of Chemistry—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaUfa Institute of Chemistry—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaKWS Gateway Research Center, Saint Louis, MO 63132, USAThe protective effects against drought stress of the endophytic bacterium <i>Bacillus subtilis</i> 10-4 were measured by studying the priming response in two wheat (<i>Triticum aestivum</i> L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought, respectively. <i>B. subtilis</i> 10-4 improved germination and growth parameters under normal conditions in both cultivars with the most pronounced effect observed in cv. E70. Under drought conditions, <i>B. subtilis</i> 10-4 significantly ameliorated the negative impact of stress on germination and growth of cv. E70, but had no protective effect on cv. SY. <i>B. subtilis</i> 10-4 induced an increase in the levels of photosynthetic chlorophyll (Chl) a, Chl b, and carotenoids (Car) in the leaves of cv. E70, both under normal and drought conditions. In cv. SY plants, bacterial inoculation decreased the contents of Chl a, Chl b, and Car under normal conditions, but pigment content were almost recovered under drought stress. <i>B. subtilis</i> 10-4 increased water holding capacity (WHC) of cv. E70 (but did not affect this parameter in cv. SY) and prevented the stress-induced decline in WHC in both cultivars. Notably, <i>B. subtilis</i> 10-4 increased endogenous salicylic acid (SA) concentration in both cultivars, especially in cv. E70. Moreover, <i>B. subtilis</i> 10-4 reduced drought-induced endogenous SA accumulation, which was correlated with the influence of endophyte on growth, indicating a possible involvement of endogenous SA in the implementation of <i>B. subtilis</i>-mediated effects in both cultivars. Overall, <i>B. subtilis</i> 10-4 inoculation was found to increase drought tolerance in seedlings of both cultivars, as evidenced by decreased lipid peroxidation, proline content, and electrolyte leakage from tissues of wheat seedlings primed with <i>B. subtilis</i> 10-4 under drought conditions.https://www.mdpi.com/2223-7747/9/12/1810PGPBendophytic <i>Bacillus subtilis</i><i>Triticum aestivum</i> L.primingdrought tolerancephotosynthetic pigments |
| spellingShingle | Oksana Lastochkina Darya Garshina Sergey Ivanov Ruslan Yuldashev Regina Khafizova Chulpan Allagulova Kristina Fedorova Azamat Avalbaev Dilara Maslennikova Massimo Bosacchi Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress Plants PGPB endophytic <i>Bacillus subtilis</i> <i>Triticum aestivum</i> L. priming drought tolerance photosynthetic pigments |
| title | Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress |
| title_full | Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress |
| title_fullStr | Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress |
| title_full_unstemmed | Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress |
| title_short | Seed Priming with Endophytic <i>Bacillus subtilis</i> Modulates Physiological Responses of Two Different <i>Triticum aestivum</i> L. Cultivars under Drought Stress |
| title_sort | seed priming with endophytic i bacillus subtilis i modulates physiological responses of two different i triticum aestivum i l cultivars under drought stress |
| topic | PGPB endophytic <i>Bacillus subtilis</i> <i>Triticum aestivum</i> L. priming drought tolerance photosynthetic pigments |
| url | https://www.mdpi.com/2223-7747/9/12/1810 |
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