Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa
The pollution of soils by heavy metals resulting from mining activities is one of the major environmental problems in North Africa. Mycorrhizoremediation using mycorrhizal fungi and adapted plant species is emerging as one of the most innovative methods to remediate heavy metal pollution. This study...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/12/2033 |
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| author | Chadlia Hachani Mohammed S. Lamhamedi Claudio Cameselle Susana Gouveia Abdenbi Zine El Abidine Damase P. Khasa Zoubeir Béjaoui |
| author_facet | Chadlia Hachani Mohammed S. Lamhamedi Claudio Cameselle Susana Gouveia Abdenbi Zine El Abidine Damase P. Khasa Zoubeir Béjaoui |
| author_sort | Chadlia Hachani |
| collection | DOAJ |
| description | The pollution of soils by heavy metals resulting from mining activities is one of the major environmental problems in North Africa. Mycorrhizoremediation using mycorrhizal fungi and adapted plant species is emerging as one of the most innovative methods to remediate heavy metal pollution. This study aims to assess the growth and the nutritional status of ectomycorrhizal <i>Pinus halepensis</i> seedlings subjected to high concentrations of Pb, Zn, and Cd for possible integration in the restoration of heavy metals contaminated sites. Ectomycorrhizal and non-ectomycorrhizal <i>P. halepensis</i> seedlings were grown in uncontaminated (control) and contaminated soils for 12 months. Growth, mineral nutrition, and heavy metal content were assessed. Results showed that ectomycorrhizae significantly improved shoot and roots dry masses of <i>P. halepensis</i> seedlings, as well as nitrogen shoot content. The absorption of Pb, Zn, and Cd was much higher in the roots than in the shoots, and significantly more pronounced in ectomycorrhizal seedlings—especially for Zn and Cd. The presence of ectomycorrhizae significantly reduced the translocation factor of Zn and Cd and bioaccumulation factor of Pb and Cd, which enhanced the phytostabilizing potential of <i>P. halepensis</i> seedlings. These results support the use of ectomycorrhizal <i>P. halepensis</i> in the remediation of heavy metal contaminated sites. |
| first_indexed | 2024-03-10T13:55:50Z |
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| id | doaj.art-119438025b6a4cb5a74beabdca5b7e97 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:55:50Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-119438025b6a4cb5a74beabdca5b7e972023-11-21T01:37:29ZengMDPI AGMicroorganisms2076-26072020-12-01812203310.3390/microorganisms8122033Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North AfricaChadlia Hachani0Mohammed S. Lamhamedi1Claudio Cameselle2Susana Gouveia3Abdenbi Zine El Abidine4Damase P. Khasa5Zoubeir Béjaoui6Faculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia and Laboratory of Forest Ecology (LR11INRGREF03), National Institute of Research in Rural Engineering, Water and Forests (INRGREF), University of Carthage, Hédi Elkarray Street, Elmenzah IV, BP 10, Ariana 2080, TunisiaCenter for Forest Studies, Faculty of Forestry, Geography and Geomatics, Abitibi Price Building, Laval University, Quebec, QC G1V 0A6, CanadaBiotecnIA, Department of Chemical Engineering, University of Vigo, Rua Maxwell s/n, Building Fundicion, 36310 Vigo, SpainBiotecnIA, Department of Chemical Engineering, University of Vigo, Rua Maxwell s/n, Building Fundicion, 36310 Vigo, SpainNational Forest School of Engineers, B.P. 5 1 1, Tabriquet, Salé 11015, MoroccoCentre for Forest Research and Institute for Systems and Integrative Biology, Université Laval, 1030 Avenue de la Médecine, Québec, QC G1V0A6, CanadaFaculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia and Laboratory of Forest Ecology (LR11INRGREF03), National Institute of Research in Rural Engineering, Water and Forests (INRGREF), University of Carthage, Hédi Elkarray Street, Elmenzah IV, BP 10, Ariana 2080, TunisiaThe pollution of soils by heavy metals resulting from mining activities is one of the major environmental problems in North Africa. Mycorrhizoremediation using mycorrhizal fungi and adapted plant species is emerging as one of the most innovative methods to remediate heavy metal pollution. This study aims to assess the growth and the nutritional status of ectomycorrhizal <i>Pinus halepensis</i> seedlings subjected to high concentrations of Pb, Zn, and Cd for possible integration in the restoration of heavy metals contaminated sites. Ectomycorrhizal and non-ectomycorrhizal <i>P. halepensis</i> seedlings were grown in uncontaminated (control) and contaminated soils for 12 months. Growth, mineral nutrition, and heavy metal content were assessed. Results showed that ectomycorrhizae significantly improved shoot and roots dry masses of <i>P. halepensis</i> seedlings, as well as nitrogen shoot content. The absorption of Pb, Zn, and Cd was much higher in the roots than in the shoots, and significantly more pronounced in ectomycorrhizal seedlings—especially for Zn and Cd. The presence of ectomycorrhizae significantly reduced the translocation factor of Zn and Cd and bioaccumulation factor of Pb and Cd, which enhanced the phytostabilizing potential of <i>P. halepensis</i> seedlings. These results support the use of ectomycorrhizal <i>P. halepensis</i> in the remediation of heavy metal contaminated sites.https://www.mdpi.com/2076-2607/8/12/2033ectomycorrhizae<i>Pinus halepensis</i>heavy metalsplant growthmineral nutritionmining |
| spellingShingle | Chadlia Hachani Mohammed S. Lamhamedi Claudio Cameselle Susana Gouveia Abdenbi Zine El Abidine Damase P. Khasa Zoubeir Béjaoui Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa Microorganisms ectomycorrhizae <i>Pinus halepensis</i> heavy metals plant growth mineral nutrition mining |
| title | Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa |
| title_full | Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa |
| title_fullStr | Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa |
| title_full_unstemmed | Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa |
| title_short | Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of <i>Pinus halepensis</i> Seedlings in North Africa |
| title_sort | effects of ectomycorrhizal fungi and heavy metals pb zn and cd on growth and mineral nutrition of i pinus halepensis i seedlings in north africa |
| topic | ectomycorrhizae <i>Pinus halepensis</i> heavy metals plant growth mineral nutrition mining |
| url | https://www.mdpi.com/2076-2607/8/12/2033 |
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