Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations

Lipase isolated from a thermoalkalophilic Pseudomonas species was used as additive to improve the degree of olive oil removal from cotton fabric in the presence of surfactants. The lipase used in this study was found to be more effective with non ionic surfactants as compared to ionic surfactants. I...

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Main Authors: Ibrahim, C. O., Khoo, M. L.
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2009-01-01
Series:Malaysian Journal of Microbiology
Subjects:
Online Access:http://web.usm.my/mjm/issues/vol5no1/research1.pdf
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author Ibrahim, C. O.
Khoo, M. L.
author_facet Ibrahim, C. O.
Khoo, M. L.
author_sort Ibrahim, C. O.
collection DOAJ
description Lipase isolated from a thermoalkalophilic Pseudomonas species was used as additive to improve the degree of olive oil removal from cotton fabric in the presence of surfactants. The lipase used in this study was found to be more effective with non ionic surfactants as compared to ionic surfactants. In terms of stability, there was no decrease in activity found in the presence of Tween 85, Span 80 and Span 20. Lipase from Pseudomonas species was most active in the presence of Tween 85, Span 80 and Span 20. The application of lipase from Pseudomonas species as an additive in the formulation containing Span 80 has improved oil removal by 36% using the washing system consisting 5 U/mL lipase, at 70 °C for 20 min and 0.8% of Span 80 as surfactant. Considering that lipase from Pseudomonas species is stable in high pH and temperatures in the presence of various surfactants, therefore it is suitable to be incorporated as additives in potential detergent formulations.
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spelling doaj.art-11958203636145969b081c426030e0542022-12-21T18:54:31ZengMalaysian Society for MicrobiologyMalaysian Journal of Microbiology1823-82622231-75382009-01-015115Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent FormulationsIbrahim, C. O.Khoo, M. L.Lipase isolated from a thermoalkalophilic Pseudomonas species was used as additive to improve the degree of olive oil removal from cotton fabric in the presence of surfactants. The lipase used in this study was found to be more effective with non ionic surfactants as compared to ionic surfactants. In terms of stability, there was no decrease in activity found in the presence of Tween 85, Span 80 and Span 20. Lipase from Pseudomonas species was most active in the presence of Tween 85, Span 80 and Span 20. The application of lipase from Pseudomonas species as an additive in the formulation containing Span 80 has improved oil removal by 36% using the washing system consisting 5 U/mL lipase, at 70 °C for 20 min and 0.8% of Span 80 as surfactant. Considering that lipase from Pseudomonas species is stable in high pH and temperatures in the presence of various surfactants, therefore it is suitable to be incorporated as additives in potential detergent formulations.http://web.usm.my/mjm/issues/vol5no1/research1.pdfPseudomonas speciesDegree of olive oil removalSpan 80Lipase
spellingShingle Ibrahim, C. O.
Khoo, M. L.
Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
Malaysian Journal of Microbiology
Pseudomonas species
Degree of olive oil removal
Span 80
Lipase
title Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
title_full Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
title_fullStr Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
title_full_unstemmed Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
title_short Lipase From Thermoalkalophilic Pseudomonas species as an Additive in Potential Laundry Detergent Formulations
title_sort lipase from thermoalkalophilic pseudomonas species as an additive in potential laundry detergent formulations
topic Pseudomonas species
Degree of olive oil removal
Span 80
Lipase
url http://web.usm.my/mjm/issues/vol5no1/research1.pdf
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