Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
BAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These wax...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1286 |
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| author | Lecheng Liu Huanhuan Xu Wanyue Zhang Jiayi Xing Mingzhao Zhu Yuchen Zhang Yongqin Wang |
| author_facet | Lecheng Liu Huanhuan Xu Wanyue Zhang Jiayi Xing Mingzhao Zhu Yuchen Zhang Yongqin Wang |
| author_sort | Lecheng Liu |
| collection | DOAJ |
| description | BAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These waxes play an essential role in resisting biotic and abiotic stresses. In this study, we identified the BAHD family in Welsh onion (<i>Allium fistulosum</i>). Our analysis revealed the presence of AfBAHDs in all chromosomes, with a distinct concentration in Chr3. Furthermore, the <i>cis</i>-acting elements of AfBAHDs were associated with abiotic/biotic stress, hormones, and light. The motif of Welsh onion BAHDs indicated the presence of a specific BAHDs motif. We also established the phylogenetic relationships of AfBAHDs, identifying three homologous genes of CER2. Subsequently, we characterized the expression of AfCER2-LIKEs in a Welsh onion mutant deficient in wax and found that AfCER2-LIKE1 plays a critical role in leaf wax metabolism, while all AfCER2-LIKEs respond to abiotic stress. Our findings provide new insights into the BAHD family and lay a foundation for future studies on the regulation of wax metabolism in Welsh onion. |
| first_indexed | 2024-03-11T02:25:05Z |
| format | Article |
| id | doaj.art-11988c77e8b24b8ca464f54ce52ed50f |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:25:05Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-11988c77e8b24b8ca464f54ce52ed50f2023-11-18T10:35:22ZengMDPI AGGenes2073-44252023-06-01146128610.3390/genes14061286Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax MetabolismLecheng Liu0Huanhuan Xu1Wanyue Zhang2Jiayi Xing3Mingzhao Zhu4Yuchen Zhang5Yongqin Wang6College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaBeijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing 100097, ChinaBeijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing 100097, ChinaBeijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing 100097, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaBeijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing 100097, ChinaBAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These waxes play an essential role in resisting biotic and abiotic stresses. In this study, we identified the BAHD family in Welsh onion (<i>Allium fistulosum</i>). Our analysis revealed the presence of AfBAHDs in all chromosomes, with a distinct concentration in Chr3. Furthermore, the <i>cis</i>-acting elements of AfBAHDs were associated with abiotic/biotic stress, hormones, and light. The motif of Welsh onion BAHDs indicated the presence of a specific BAHDs motif. We also established the phylogenetic relationships of AfBAHDs, identifying three homologous genes of CER2. Subsequently, we characterized the expression of AfCER2-LIKEs in a Welsh onion mutant deficient in wax and found that AfCER2-LIKE1 plays a critical role in leaf wax metabolism, while all AfCER2-LIKEs respond to abiotic stress. Our findings provide new insights into the BAHD family and lay a foundation for future studies on the regulation of wax metabolism in Welsh onion.https://www.mdpi.com/2073-4425/14/6/1286BAHD acyltransferaseCER2-LIKEsgenes familyabiotic stressepicuticular wax |
| spellingShingle | Lecheng Liu Huanhuan Xu Wanyue Zhang Jiayi Xing Mingzhao Zhu Yuchen Zhang Yongqin Wang Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism Genes BAHD acyltransferase CER2-LIKEs genes family abiotic stress epicuticular wax |
| title | Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism |
| title_full | Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism |
| title_fullStr | Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism |
| title_full_unstemmed | Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism |
| title_short | Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism |
| title_sort | genome wide analysis of the bahd family in welsh onion and cer2 likes involved in wax metabolism |
| topic | BAHD acyltransferase CER2-LIKEs genes family abiotic stress epicuticular wax |
| url | https://www.mdpi.com/2073-4425/14/6/1286 |
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