Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds
The present study describes the anti-biofilm and quorum sensing (QS) inhibitory potential of extracts and chemical constituents from <i>Piper bogotense</i>. Antibiofilm potential was determined through crystal violet assay against <i>Pseudomonas aeruginosa</i>, while QS inhib...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/9/1901 |
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| author | Andrés G. Sierra-Quitian Lida V. Hernandez-Moreno Ludy C. Pabon-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino |
| author_facet | Andrés G. Sierra-Quitian Lida V. Hernandez-Moreno Ludy C. Pabon-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino |
| author_sort | Andrés G. Sierra-Quitian |
| collection | DOAJ |
| description | The present study describes the anti-biofilm and quorum sensing (QS) inhibitory potential of extracts and chemical constituents from <i>Piper bogotense</i>. Antibiofilm potential was determined through crystal violet assay against <i>Pseudomonas aeruginosa</i>, while QS inhibition efficacy was determined through violacein inhibition assay using <i>Chromobacterium violaceum</i> as a bacterial model. Additionally, this study reports the effects of the chemical constituents isolated in <i>P. bogotense</i> against various virulent factors associated with QS, such as the percentage decrease in pyocyanin, elastase, and protease production. The chemical study led to the isolation and identification of two prenylated benzoic acids (<b>1</b> and <b>2</b>) and a prenylated hydroquinone <b>3</b>, of which compounds <b>1</b> and <b>2</b> are reported for the first time for <i>P. bogotense</i>. The ethanolic extract and the DCM fraction from <i>P. bogotense</i> stand out for reducing violacein production in <i>C. violaceum</i>, as well as the biofilm formation in <i>P. aeruginosa</i>. Compounds <b>2</b> and <b>3</b> stand out for having the lowest violacein production (43.8% and 68.3%), as well as the lowest production of virulence factors such as elastase (60.2% and 51.4%) and pyocyanin (39.7% and 33.2%). These results demonstrate the potential of <i>P. bogotense</i> components to be used as an alternative control against multidrug-resistant <i>P. aeruginosa</i>. |
| first_indexed | 2024-03-11T04:08:47Z |
| format | Article |
| id | doaj.art-119fb0229b73434585a6c9a4a158381c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:08:47Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-119fb0229b73434585a6c9a4a158381c2023-11-17T23:33:15ZengMDPI AGPlants2223-77472023-05-01129190110.3390/plants12091901Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive CompoundsAndrés G. Sierra-Quitian0Lida V. Hernandez-Moreno1Ludy C. Pabon-Baquero2Juliet A. Prieto-Rodriguez3Oscar J. Patiño-Ladino4Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Bogotá 111321, ColombiaDepartamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Bogotá 111321, ColombiaEscuela de Ciencias Básicas y Aplicadas, Universidad de La Salle, Bogotá 111711, ColombiaDepartamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaDepartamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Bogotá 111321, ColombiaThe present study describes the anti-biofilm and quorum sensing (QS) inhibitory potential of extracts and chemical constituents from <i>Piper bogotense</i>. Antibiofilm potential was determined through crystal violet assay against <i>Pseudomonas aeruginosa</i>, while QS inhibition efficacy was determined through violacein inhibition assay using <i>Chromobacterium violaceum</i> as a bacterial model. Additionally, this study reports the effects of the chemical constituents isolated in <i>P. bogotense</i> against various virulent factors associated with QS, such as the percentage decrease in pyocyanin, elastase, and protease production. The chemical study led to the isolation and identification of two prenylated benzoic acids (<b>1</b> and <b>2</b>) and a prenylated hydroquinone <b>3</b>, of which compounds <b>1</b> and <b>2</b> are reported for the first time for <i>P. bogotense</i>. The ethanolic extract and the DCM fraction from <i>P. bogotense</i> stand out for reducing violacein production in <i>C. violaceum</i>, as well as the biofilm formation in <i>P. aeruginosa</i>. Compounds <b>2</b> and <b>3</b> stand out for having the lowest violacein production (43.8% and 68.3%), as well as the lowest production of virulence factors such as elastase (60.2% and 51.4%) and pyocyanin (39.7% and 33.2%). These results demonstrate the potential of <i>P. bogotense</i> components to be used as an alternative control against multidrug-resistant <i>P. aeruginosa</i>.https://www.mdpi.com/2223-7747/12/9/1901<i>Piper bogotense</i>piperaceae<i>Pseudomonas aeruginosa</i>virulence factorsbiofilm formationquorum sensing |
| spellingShingle | Andrés G. Sierra-Quitian Lida V. Hernandez-Moreno Ludy C. Pabon-Baquero Juliet A. Prieto-Rodriguez Oscar J. Patiño-Ladino Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds Plants <i>Piper bogotense</i> piperaceae <i>Pseudomonas aeruginosa</i> virulence factors biofilm formation quorum sensing |
| title | Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds |
| title_full | Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds |
| title_fullStr | Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds |
| title_full_unstemmed | Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds |
| title_short | Antiquorum and Antibiofilm Activities of <i>Piper bogotense</i> C. DC. against <i>Pseudomonas aeruginosa</i> and Identification of Bioactive Compounds |
| title_sort | antiquorum and antibiofilm activities of i piper bogotense i c dc against i pseudomonas aeruginosa i and identification of bioactive compounds |
| topic | <i>Piper bogotense</i> piperaceae <i>Pseudomonas aeruginosa</i> virulence factors biofilm formation quorum sensing |
| url | https://www.mdpi.com/2223-7747/12/9/1901 |
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