A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit.
Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3326016?pdf=render |
| _version_ | 1818327574910074880 |
|---|---|
| author | Rosa L López-Marqués Lisbeth R Poulsen Michael G Palmgren |
| author_facet | Rosa L López-Marqués Lisbeth R Poulsen Michael G Palmgren |
| author_sort | Rosa L López-Marqués |
| collection | DOAJ |
| description | Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit. |
| first_indexed | 2024-12-13T12:18:26Z |
| format | Article |
| id | doaj.art-119fdb154a544db5922872bb825ea848 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T12:18:26Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-119fdb154a544db5922872bb825ea8482022-12-21T23:46:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3304210.1371/journal.pone.0033042A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit.Rosa L López-MarquésLisbeth R PoulsenMichael G PalmgrenPlasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.http://europepmc.org/articles/PMC3326016?pdf=render |
| spellingShingle | Rosa L López-Marqués Lisbeth R Poulsen Michael G Palmgren A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. PLoS ONE |
| title | A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. |
| title_full | A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. |
| title_fullStr | A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. |
| title_full_unstemmed | A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. |
| title_short | A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a β-subunit. |
| title_sort | putative plant aminophospholipid flippase the arabidopsis p4 atpase ala1 localizes to the plasma membrane following association with a β subunit |
| url | http://europepmc.org/articles/PMC3326016?pdf=render |
| work_keys_str_mv | AT rosallopezmarques aputativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit AT lisbethrpoulsen aputativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit AT michaelgpalmgren aputativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit AT rosallopezmarques putativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit AT lisbethrpoulsen putativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit AT michaelgpalmgren putativeplantaminophospholipidflippasethearabidopsisp4atpaseala1localizestotheplasmamembranefollowingassociationwithabsubunit |