Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells
Areca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardiza...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2008-01-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/304 |
| _version_ | 1818652559801319424 |
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| author | Edy Meiyanto Ratna Asmah Susidarti Sri Handayani Fitria Rahmi |
| author_facet | Edy Meiyanto Ratna Asmah Susidarti Sri Handayani Fitria Rahmi |
| author_sort | Edy Meiyanto |
| collection | DOAJ |
| description | Areca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardization was done according to the standard of BPOM. Areca catechu seed powder extraction was done using ethanol 96%. Cytotoxic assay – to get the value of IC50 and to prevent the cell proliferation (using doubling time assay) – was carried out by using MTT assay. Apoptosis observation was done by acrydine orange- etidium bromide staining method (double staining). The result showed that treatment with Areca catechu seed ethanolic extract (25-100 µg/m) for 48 h caused 13-84% growth inhibition (IC 5077 µg/mL) of the cells, while arecoline (ARE) treatment (10-500 µg/mL) showed 8-73% inhibition (IC50 180 µg/mL). The extract also inhibited cell proliferation and induced apoptosis. These results conclude that Areca catechu seed ethanolic extract (EP) possesses antiproliferative effect through growth inhibition and apoptosis induction. |
| first_indexed | 2024-12-17T02:23:56Z |
| format | Article |
| id | doaj.art-11a160d9e73842a1a72cacc763721029 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-17T02:23:56Z |
| publishDate | 2008-01-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-11a160d9e73842a1a72cacc7637210292022-12-21T22:07:10ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862008-01-011911219http://dx.doi.org/10.14499/indonesianjpharm0iss0pp12-19Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cellsEdy Meiyanto0Ratna Asmah Susidarti1Sri Handayani2Fitria Rahmi3Fakultas Farmasi Universitas Gadjah Mada YogyakartaFakultas Farmasi Universitas Gadjah Mada YogyakartaFakultas Farmasi Universitas Gadjah Mada YogyakartaBadan POM-JakartaAreca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardization was done according to the standard of BPOM. Areca catechu seed powder extraction was done using ethanol 96%. Cytotoxic assay – to get the value of IC50 and to prevent the cell proliferation (using doubling time assay) – was carried out by using MTT assay. Apoptosis observation was done by acrydine orange- etidium bromide staining method (double staining). The result showed that treatment with Areca catechu seed ethanolic extract (25-100 µg/m) for 48 h caused 13-84% growth inhibition (IC 5077 µg/mL) of the cells, while arecoline (ARE) treatment (10-500 µg/mL) showed 8-73% inhibition (IC50 180 µg/mL). The extract also inhibited cell proliferation and induced apoptosis. These results conclude that Areca catechu seed ethanolic extract (EP) possesses antiproliferative effect through growth inhibition and apoptosis induction.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/304MCF-7Areca catechuantiproliferative |
| spellingShingle | Edy Meiyanto Ratna Asmah Susidarti Sri Handayani Fitria Rahmi Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells Indonesian Journal of Pharmacy MCF-7 Areca catechu antiproliferative |
| title | Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells |
| title_full | Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells |
| title_fullStr | Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells |
| title_full_unstemmed | Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells |
| title_short | Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells |
| title_sort | ethanolic extract of areca catechu seeds inhibit proliferation and induce apoptosis on mcf 7 cells |
| topic | MCF-7 Areca catechu antiproliferative |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/304 |
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