Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function
Regulatory T cells (Tregs) play a major role in the prevention of autoimmune diseases. Transfer of Foxp3 gene into conventional T cells converts their phenotype to regulatory T cells. Therefore, the question arises as to whether adoptively transferred in vitro differentiated Treg cells specific for...
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| Format: | Article |
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Tehran University of Medical Sciences
2018-08-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
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| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1609 |
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| author | Mahdi Zavvar Mohsen Abdolmaleki Hamid Farajifard Farshid Noorbakhsh kayhan Azadmanesh Mohammad Vojgani Mohammad Hossein Nikcnam |
| author_facet | Mahdi Zavvar Mohsen Abdolmaleki Hamid Farajifard Farshid Noorbakhsh kayhan Azadmanesh Mohammad Vojgani Mohammad Hossein Nikcnam |
| author_sort | Mahdi Zavvar |
| collection | DOAJ |
| description | Regulatory T cells (Tregs) play a major role in the prevention of autoimmune diseases. Transfer of Foxp3 gene into conventional T cells converts their phenotype to regulatory T cells. Therefore, the question arises as to whether adoptively transferred in vitro differentiated Treg cells specific for a locally expressed antigen might have better inhibitory effects on the progression of the disease as compared with antigen-nonspecific T reg cells. Herein, we investigated the therapeutic potential of primed and unprimed retrovirus mediated Foxp3-overexpression T cells following intravenously injected of these cells into affected rats with collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. Our analyses demonstrate that systemic administration of collagen II primed Foxp3-transduced T cells could markedly ameliorate CIA inflammatory responses at clinical (p<0.0014) and pathological exchanges including cellular infiltration (p=0.002), bone erosion (p=0.0013) and synovial hyperplasia (p=0.002). In contrast, collagen II unprimed Foxp3-transduced T cells like as collagen II primed or unprimed GFP-transduced T cells did not reveal any beneficial effects on arthritis features as compared with untreated group (p>0.05). Therefore, we believe that collagen II primed Foxp3-transduced T cells are interacting locally and systemically with immune cells which reveled with decreasing of T cells infiltration into joints along with specific CII IgG production. Considering the results described here, it appears that the using patients' T cells which previously exposed to specific antigens may have more effective therapeutic advantage in the production of induced regulatory T cells in the treatment of arthritis. |
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| id | doaj.art-11a266427cc642fb8b2066dabdcd5a1b |
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| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-12T22:00:40Z |
| publishDate | 2018-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-11a266427cc642fb8b2066dabdcd5a1b2022-12-22T00:10:31ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492018-08-0117410.18502/ijaai.v17i4.951609Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell FunctionMahdi Zavvar0Mohsen Abdolmaleki1Hamid Farajifard2Farshid Noorbakhsh3kayhan Azadmanesh4Mohammad Vojgani5Mohammad Hossein Nikcnam6Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Virology, Pasteur Institute of Iran, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Molecular Immunology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, IranRegulatory T cells (Tregs) play a major role in the prevention of autoimmune diseases. Transfer of Foxp3 gene into conventional T cells converts their phenotype to regulatory T cells. Therefore, the question arises as to whether adoptively transferred in vitro differentiated Treg cells specific for a locally expressed antigen might have better inhibitory effects on the progression of the disease as compared with antigen-nonspecific T reg cells. Herein, we investigated the therapeutic potential of primed and unprimed retrovirus mediated Foxp3-overexpression T cells following intravenously injected of these cells into affected rats with collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. Our analyses demonstrate that systemic administration of collagen II primed Foxp3-transduced T cells could markedly ameliorate CIA inflammatory responses at clinical (p<0.0014) and pathological exchanges including cellular infiltration (p=0.002), bone erosion (p=0.0013) and synovial hyperplasia (p=0.002). In contrast, collagen II unprimed Foxp3-transduced T cells like as collagen II primed or unprimed GFP-transduced T cells did not reveal any beneficial effects on arthritis features as compared with untreated group (p>0.05). Therefore, we believe that collagen II primed Foxp3-transduced T cells are interacting locally and systemically with immune cells which reveled with decreasing of T cells infiltration into joints along with specific CII IgG production. Considering the results described here, it appears that the using patients' T cells which previously exposed to specific antigens may have more effective therapeutic advantage in the production of induced regulatory T cells in the treatment of arthritis.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1609Collagen-induced arthritisRetroviral vectorsRheumatoid arthritisT regulatory cells |
| spellingShingle | Mahdi Zavvar Mohsen Abdolmaleki Hamid Farajifard Farshid Noorbakhsh kayhan Azadmanesh Mohammad Vojgani Mohammad Hossein Nikcnam Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function Iranian Journal of Allergy, Asthma and Immunology Collagen-induced arthritis Retroviral vectors Rheumatoid arthritis T regulatory cells |
| title | Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function |
| title_full | Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function |
| title_fullStr | Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function |
| title_full_unstemmed | Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function |
| title_short | Collagen II-primed Foxp3 Transduced T Cells Ameliorate Collagen-induced Arthritis in Rats: The Effect of Antigenic Priming on T Regulatory Cell Function |
| title_sort | collagen ii primed foxp3 transduced t cells ameliorate collagen induced arthritis in rats the effect of antigenic priming on t regulatory cell function |
| topic | Collagen-induced arthritis Retroviral vectors Rheumatoid arthritis T regulatory cells |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1609 |
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