Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues
Abstract Background Panax quinquefolius L. (American ginseng) is widely used in medicine due to its wealth of diverse pharmacological effects. Endophytes colonize within P. quinquefolius in multiple tissue types. However, the relationship between endophytes and the production of their active ingredi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04282-z |
| _version_ | 1797789826021326848 |
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| author | Rui Li Wanying Duan Zhifang Ran Xiaoli Chen Hongxia Yu Lei Fang Lanping Guo Jie Zhou |
| author_facet | Rui Li Wanying Duan Zhifang Ran Xiaoli Chen Hongxia Yu Lei Fang Lanping Guo Jie Zhou |
| author_sort | Rui Li |
| collection | DOAJ |
| description | Abstract Background Panax quinquefolius L. (American ginseng) is widely used in medicine due to its wealth of diverse pharmacological effects. Endophytes colonize within P. quinquefolius in multiple tissue types. However, the relationship between endophytes and the production of their active ingredients in different parts of the plant is not clear. Results In this study, the relationship of endophytic diversity and the metabolites produced in different plant tissues of P. quinquefolius were analyzed using metagenomic and metabolomic approaches. The results showed relatively similar endophyte composition in roots and fibrils, but obvious differences between endophyte populations in stems and leaves. Species abundance analysis showed that at the phylum level, the dominant bacterial phylum was Cyanobacteria for roots, fibrils, stems and leaves, Ascomycota forroots and fibrils roots, and Basidiomycota for stems and leaves. LC-MS/MS technology was used to quantitatively analyze the metabolites in different tissues of P. quinquefolius. A total of 398 metabolites and 294 differential metaboliteswere identified, mainly organic acids, sugars, amino acids, polyphenols, and saponins. Most of the differential metabolites were enriched in metabolic pathways such as phenylpropane biosynthesis, flavonoid biosynthesis, citric acid cycle, and amino acid biosynthesis. Correlation analysis showed a positive and negative correlation between the endophytes and the differential metabolites. Conexibacter significantly enriched in root and fibril was significantly positively correlated with saponin differential metabolites, while cyberlindnera significantly enriched in stem and leaf was significantly negatively correlated with differential metabolites (p < 0.05). Conclusion The endophytic communities diversity were relatively similar in the roots and fibrils of P. quinquefolius, while there were greater differences between the stems and leaves. There was significant difference in metabolite content between different tissues of P. quinquefolius. Correlation analysis methods demonstrated a correlation between endophytes and differential metabolism. |
| first_indexed | 2024-03-13T01:56:05Z |
| format | Article |
| id | doaj.art-11a2a9127aaa474ab3ce3aee0a98e55e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T01:56:05Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-11a2a9127aaa474ab3ce3aee0a98e55e2023-07-02T11:12:00ZengBMCBMC Plant Biology1471-22292023-05-0123111510.1186/s12870-023-04282-zDiversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissuesRui Li0Wanying Duan1Zhifang Ran2Xiaoli Chen3Hongxia Yu4Lei Fang5Lanping Guo6Jie Zhou7School of Biological Science and Technology, University of JinanSchool of Biological Science and Technology, University of JinanSchool of Biological Science and Technology, University of JinanSchool of Biological Science and Technology, University of JinanWeihai Wendeng District Dao-di Ginseng Industry Development Co. LTDSchool of Biological Science and Technology, University of JinanState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Medica, China Academy of Chinese Medical SciencesSchool of Biological Science and Technology, University of JinanAbstract Background Panax quinquefolius L. (American ginseng) is widely used in medicine due to its wealth of diverse pharmacological effects. Endophytes colonize within P. quinquefolius in multiple tissue types. However, the relationship between endophytes and the production of their active ingredients in different parts of the plant is not clear. Results In this study, the relationship of endophytic diversity and the metabolites produced in different plant tissues of P. quinquefolius were analyzed using metagenomic and metabolomic approaches. The results showed relatively similar endophyte composition in roots and fibrils, but obvious differences between endophyte populations in stems and leaves. Species abundance analysis showed that at the phylum level, the dominant bacterial phylum was Cyanobacteria for roots, fibrils, stems and leaves, Ascomycota forroots and fibrils roots, and Basidiomycota for stems and leaves. LC-MS/MS technology was used to quantitatively analyze the metabolites in different tissues of P. quinquefolius. A total of 398 metabolites and 294 differential metaboliteswere identified, mainly organic acids, sugars, amino acids, polyphenols, and saponins. Most of the differential metabolites were enriched in metabolic pathways such as phenylpropane biosynthesis, flavonoid biosynthesis, citric acid cycle, and amino acid biosynthesis. Correlation analysis showed a positive and negative correlation between the endophytes and the differential metabolites. Conexibacter significantly enriched in root and fibril was significantly positively correlated with saponin differential metabolites, while cyberlindnera significantly enriched in stem and leaf was significantly negatively correlated with differential metabolites (p < 0.05). Conclusion The endophytic communities diversity were relatively similar in the roots and fibrils of P. quinquefolius, while there were greater differences between the stems and leaves. There was significant difference in metabolite content between different tissues of P. quinquefolius. Correlation analysis methods demonstrated a correlation between endophytes and differential metabolism.https://doi.org/10.1186/s12870-023-04282-zPanax quinquefolius LEndophytesVarious tissuesDiversitySecondary metabolitesCorrelation analysis |
| spellingShingle | Rui Li Wanying Duan Zhifang Ran Xiaoli Chen Hongxia Yu Lei Fang Lanping Guo Jie Zhou Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues BMC Plant Biology Panax quinquefolius L Endophytes Various tissues Diversity Secondary metabolites Correlation analysis |
| title | Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues |
| title_full | Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues |
| title_fullStr | Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues |
| title_full_unstemmed | Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues |
| title_short | Diversity and correlation analysis of endophytes and metabolites of Panax quinquefolius L. in various tissues |
| title_sort | diversity and correlation analysis of endophytes and metabolites of panax quinquefolius l in various tissues |
| topic | Panax quinquefolius L Endophytes Various tissues Diversity Secondary metabolites Correlation analysis |
| url | https://doi.org/10.1186/s12870-023-04282-z |
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