Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4
Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5’ cap and a 3’ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5’ cap formation. To learn more about the replication complex of BaMV, a protein...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01508/full |
| _version_ | 1811222400328531968 |
|---|---|
| author | Cheng-Cheng eLee Tzu-Ling eLin Jhe-Wei eLin Yu-Tsung eHan Yu-Ting eHuang Yau-Heiu eHsu Menghsiao eMeng |
| author_facet | Cheng-Cheng eLee Tzu-Ling eLin Jhe-Wei eLin Yu-Tsung eHan Yu-Ting eHuang Yau-Heiu eHsu Menghsiao eMeng |
| author_sort | Cheng-Cheng eLee |
| collection | DOAJ |
| description | Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5’ cap and a 3’ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5’ cap formation. To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. Among them, the cytoplasmic exoribonuclease NbXRN4 particularly caught our attention. NbXRN4 has been shown to have an antiviral activity against Tomato bushy stunt virus and Tomato mosaic virus. In Arabidopsis, the enzyme could reduce RNAi- and miRNA-mediated RNA decay. This study found that downregulation of NbXRN4 greatly decreased BaMV accumulation, while overexpression of NbXRN4 resulted in an opposite effect. Mutations at the catalytically essential residues abolished the function of NbXRN4 in the increase of BaMV accumulation. Nonetheless, NbXRN4 was still able to promote BaMV accumulation in the presence of the RNA silencing suppressor P19. In summary, the replication efficiency of BaMV may be improved by the exoribonuclease activity of NbXRN4. |
| first_indexed | 2024-04-12T08:15:13Z |
| format | Article |
| id | doaj.art-11a33998fb494513859c432e6487fc88 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T08:15:13Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-11a33998fb494513859c432e6487fc882022-12-22T03:40:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-01-01610.3389/fmicb.2015.01508171527Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4Cheng-Cheng eLee0Tzu-Ling eLin1Jhe-Wei eLin2Yu-Tsung eHan3Yu-Ting eHuang4Yau-Heiu eHsu5Menghsiao eMeng6National Chung Hsing UniversityNational Chung Hsing UniversityNational Chung Hsing UniversityNational Chung Hsing UniversityNational Chung Hsing UniversityNational Chung Hsing UniversityNational Chung Hsing UniversityBamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5’ cap and a 3’ poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5’ cap formation. To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. Among them, the cytoplasmic exoribonuclease NbXRN4 particularly caught our attention. NbXRN4 has been shown to have an antiviral activity against Tomato bushy stunt virus and Tomato mosaic virus. In Arabidopsis, the enzyme could reduce RNAi- and miRNA-mediated RNA decay. This study found that downregulation of NbXRN4 greatly decreased BaMV accumulation, while overexpression of NbXRN4 resulted in an opposite effect. Mutations at the catalytically essential residues abolished the function of NbXRN4 in the increase of BaMV accumulation. Nonetheless, NbXRN4 was still able to promote BaMV accumulation in the presence of the RNA silencing suppressor P19. In summary, the replication efficiency of BaMV may be improved by the exoribonuclease activity of NbXRN4.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01508/fullPotexvirusRNA replicationhost factorsReplicaseExonucleaseXRN4 |
| spellingShingle | Cheng-Cheng eLee Tzu-Ling eLin Jhe-Wei eLin Yu-Tsung eHan Yu-Ting eHuang Yau-Heiu eHsu Menghsiao eMeng Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 Frontiers in Microbiology Potexvirus RNA replication host factors Replicase Exonuclease XRN4 |
| title | Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 |
| title_full | Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 |
| title_fullStr | Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 |
| title_full_unstemmed | Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 |
| title_short | Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 |
| title_sort | promotion of bamboo mosaic virus accumulation in nicotiana benthamiana by 5 3 exonuclease nbxrn4 |
| topic | Potexvirus RNA replication host factors Replicase Exonuclease XRN4 |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01508/full |
| work_keys_str_mv | AT chengchengelee promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT tzulingelin promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT jheweielin promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT yutsungehan promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT yutingehuang promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT yauheiuehsu promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 AT menghsiaoemeng promotionofbamboomosaicvirusaccumulationinnicotianabenthamianaby53exonucleasenbxrn4 |