An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association

Abstract Endothelial cells and pericytes are highly dynamic vascular cells and several subtypes, based on their spatiotemporal dynamics or molecular expression, are believed to exist. The interaction between endothelial cells and pericytes is of importance in many aspects ranging from basic developm...

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Main Authors: Ann L. B. Seynhaeve, Timo L. M. ten Hagen
Format: Article
Language:English
Published: Nature Portfolio 2021-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-99939-w
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author Ann L. B. Seynhaeve
Timo L. M. ten Hagen
author_facet Ann L. B. Seynhaeve
Timo L. M. ten Hagen
author_sort Ann L. B. Seynhaeve
collection DOAJ
description Abstract Endothelial cells and pericytes are highly dynamic vascular cells and several subtypes, based on their spatiotemporal dynamics or molecular expression, are believed to exist. The interaction between endothelial cells and pericytes is of importance in many aspects ranging from basic development to diseases like cancer. Identification of spatiotemporal dynamics is particularly interesting and methods to studies these are in demand. Here we describe the technical details of a method combining the benefits of high resolution intravital imaging and whole-mount histology. With intravital imaging using an adapted light weight dorsal skinfold chamber we identified blood flow patterns and spatiotemporal subtypes of endothelial cells and pericytes in a 4D (XYZ, spatial+T, time dimension) manner as representative examples for this model. Thereafter the tissue was extracted and stained as a whole-mount, by which the position and volumetric space of endothelial cells as well as pericytes were maintained, to identify molecular subtypes. Integration of the two imaging methods enabled 4D dissection of endothelial cell–pericyte association at the molecular level.
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spelling doaj.art-11a3f948694a43929e02d6ec208222fe2022-12-21T20:09:15ZengNature PortfolioScientific Reports2045-23222021-10-0111111410.1038/s41598-021-99939-wAn adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte associationAnn L. B. Seynhaeve0Timo L. M. ten Hagen1Laboratory Experimental Oncology, Department of Pathology, Erasmus MCLaboratory Experimental Oncology, Department of Pathology, Erasmus MCAbstract Endothelial cells and pericytes are highly dynamic vascular cells and several subtypes, based on their spatiotemporal dynamics or molecular expression, are believed to exist. The interaction between endothelial cells and pericytes is of importance in many aspects ranging from basic development to diseases like cancer. Identification of spatiotemporal dynamics is particularly interesting and methods to studies these are in demand. Here we describe the technical details of a method combining the benefits of high resolution intravital imaging and whole-mount histology. With intravital imaging using an adapted light weight dorsal skinfold chamber we identified blood flow patterns and spatiotemporal subtypes of endothelial cells and pericytes in a 4D (XYZ, spatial+T, time dimension) manner as representative examples for this model. Thereafter the tissue was extracted and stained as a whole-mount, by which the position and volumetric space of endothelial cells as well as pericytes were maintained, to identify molecular subtypes. Integration of the two imaging methods enabled 4D dissection of endothelial cell–pericyte association at the molecular level.https://doi.org/10.1038/s41598-021-99939-w
spellingShingle Ann L. B. Seynhaeve
Timo L. M. ten Hagen
An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
Scientific Reports
title An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
title_full An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
title_fullStr An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
title_full_unstemmed An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
title_short An adapted dorsal skinfold model used for 4D intravital followed by whole-mount imaging to reveal endothelial cell–pericyte association
title_sort adapted dorsal skinfold model used for 4d intravital followed by whole mount imaging to reveal endothelial cell pericyte association
url https://doi.org/10.1038/s41598-021-99939-w
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