Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model
Objective: This study aimed to explore whether carvacrol (CV) had a protective effect on paclitaxel-induced ototoxicity from biochemical, functional, and histopathological perspectives.Methods: Forty Wistar albino male rats were randomly separated into five groups of eight rats. Group 1 was the cont...
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| Format: | Article |
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Galenos Yayincilik
2020-12-01
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| Series: | Turkish Archives of Otorhinolaryngology |
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http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-effect-of-carvacrol-against-paclitaxel-/43865
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| author | Fatma Atalay Arzu Tatar Büşra Dincer Betül Gündoğdu Sinan Köyceğiz |
| author_facet | Fatma Atalay Arzu Tatar Büşra Dincer Betül Gündoğdu Sinan Köyceğiz |
| author_sort | Fatma Atalay |
| collection | DOAJ |
| description | Objective: This study aimed to explore whether carvacrol (CV) had a protective effect on paclitaxel-induced ototoxicity from biochemical, functional, and histopathological perspectives.Methods: Forty Wistar albino male rats were randomly separated into five groups of eight rats. Group 1 was the control group, so Paclitaxel or CV was not administered. Group 2 was administered i.p. CV at 25 mg/kg once a week; Group 3, was administered i.p. paclitaxel at 5 mg/kg once a week; Group 4 was administered i.p. paclitaxel at 5 mg/kg followed (30 min later) by CV at 25 mg/kg once a week; and Group 5 was administered i.p. CV at 25 mg/kg followed (1 day later) by paclitaxel at 5 mg/kg. once a week. The drugs were administered intraperitoneally once a week for four consecutive weeks, and distortion product otoacoustic emissions (DPOAE) tests were performed at the beginning of the study before the first drug administration and at the end of the study after the last drug administration. All rats were sacrificed, and cochleae were removed for biochemical and histopathological analysis.Results: Biochemical data indicated that paclitaxel caused oxidative stress in the cochlea. Histopathological findings revealed the loss of outer hair cells in the organ of Corti (CO) and moderate degenerative changes in the stria vascularis (SV). It was observed that DPOAE measurements were significantly reduced at high frequencies. In groups which CV was administered together with paclitaxel, these biochemical, histopathological, and functional changes were favorably reversed.Conclusion: CV may have a protective effect against paclitaxel-induced ototoxicity when given. |
| first_indexed | 2024-04-10T11:40:25Z |
| format | Article |
| id | doaj.art-11a50196f81a485888a76cf41abd5305 |
| institution | Directory Open Access Journal |
| issn | 2667-7474 |
| language | English |
| last_indexed | 2024-04-10T11:40:25Z |
| publishDate | 2020-12-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Turkish Archives of Otorhinolaryngology |
| spelling | doaj.art-11a50196f81a485888a76cf41abd53052023-02-15T16:17:34ZengGalenos YayincilikTurkish Archives of Otorhinolaryngology2667-74742020-12-0158424124810.5152/tao.2020.571413049054Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat ModelFatma Atalay0Arzu Tatar1Büşra Dincer2Betül Gündoğdu3Sinan Köyceğiz4 Department of Otorhinolaryngology, Atatürk University School of Medicine, Erzurum, Turkey Department of Otorhinolaryngology, Atatürk University School of Medicine, Erzurum, Turkey Department of Pharmacology, Erzincan Binali Yıldırım University School of Pharmacy, Erzincan, Turkey Department of Pathology, Atatürk University School of Medicine, Erzurum, Turkey Clinic of Otorhinolaryngology, Mareşal Çakmak State Hospital, Erzurum, Turkey Objective: This study aimed to explore whether carvacrol (CV) had a protective effect on paclitaxel-induced ototoxicity from biochemical, functional, and histopathological perspectives.Methods: Forty Wistar albino male rats were randomly separated into five groups of eight rats. Group 1 was the control group, so Paclitaxel or CV was not administered. Group 2 was administered i.p. CV at 25 mg/kg once a week; Group 3, was administered i.p. paclitaxel at 5 mg/kg once a week; Group 4 was administered i.p. paclitaxel at 5 mg/kg followed (30 min later) by CV at 25 mg/kg once a week; and Group 5 was administered i.p. CV at 25 mg/kg followed (1 day later) by paclitaxel at 5 mg/kg. once a week. The drugs were administered intraperitoneally once a week for four consecutive weeks, and distortion product otoacoustic emissions (DPOAE) tests were performed at the beginning of the study before the first drug administration and at the end of the study after the last drug administration. All rats were sacrificed, and cochleae were removed for biochemical and histopathological analysis.Results: Biochemical data indicated that paclitaxel caused oxidative stress in the cochlea. Histopathological findings revealed the loss of outer hair cells in the organ of Corti (CO) and moderate degenerative changes in the stria vascularis (SV). It was observed that DPOAE measurements were significantly reduced at high frequencies. In groups which CV was administered together with paclitaxel, these biochemical, histopathological, and functional changes were favorably reversed.Conclusion: CV may have a protective effect against paclitaxel-induced ototoxicity when given. http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-effect-of-carvacrol-against-paclitaxel-/43865 carvacrolototoxicityoxidative stresspaclitaxelanimal experimentation |
| spellingShingle | Fatma Atalay Arzu Tatar Büşra Dincer Betül Gündoğdu Sinan Köyceğiz Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model Turkish Archives of Otorhinolaryngology carvacrol ototoxicity oxidative stress paclitaxel animal experimentation |
| title | Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model |
| title_full | Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model |
| title_fullStr | Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model |
| title_full_unstemmed | Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model |
| title_short | Protective Effect of Carvacrol against Paclitaxel-Induced Ototoxicity in Rat Model |
| title_sort | protective effect of carvacrol against paclitaxel induced ototoxicity in rat model |
| topic | carvacrol ototoxicity oxidative stress paclitaxel animal experimentation |
| url |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/protective-effect-of-carvacrol-against-paclitaxel-/43865
|
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