A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain
Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad rang...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2009-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/1/3/1204/ |
| _version_ | 1818157893831098368 |
|---|---|
| author | Theo P. Sloots Michael D. Nissen Graeme R. Nimmo Stephen B. Lambert Cassandra E. Faux Cheryl Bletchly Seweryn Bialasiewicz David M. Whiley Rebecca J. Rockett |
| author_facet | Theo P. Sloots Michael D. Nissen Graeme R. Nimmo Stephen B. Lambert Cassandra E. Faux Cheryl Bletchly Seweryn Bialasiewicz David M. Whiley Rebecca J. Rockett |
| author_sort | Theo P. Sloots |
| collection | DOAJ |
| description | Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples. |
| first_indexed | 2024-12-11T15:21:26Z |
| format | Article |
| id | doaj.art-11a7f7a58ece41b3b4dee94ef9c1aca3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T15:21:26Z |
| publishDate | 2009-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-11a7f7a58ece41b3b4dee94ef9c1aca32022-12-22T01:00:21ZengMDPI AGViruses1999-49152009-12-01131204120810.3390/v1031204A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) StrainTheo P. SlootsMichael D. NissenGraeme R. NimmoStephen B. LambertCassandra E. FauxCheryl BletchlySeweryn BialasiewiczDavid M. WhileyRebecca J. RockettTimely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.http://www.mdpi.com/1999-4915/1/3/1204/influenzaH1N1real-timePCRdetection |
| spellingShingle | Theo P. Sloots Michael D. Nissen Graeme R. Nimmo Stephen B. Lambert Cassandra E. Faux Cheryl Bletchly Seweryn Bialasiewicz David M. Whiley Rebecca J. Rockett A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain Viruses influenza H1N1 real-time PCR detection |
| title | A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain |
| title_full | A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain |
| title_fullStr | A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain |
| title_full_unstemmed | A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain |
| title_short | A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain |
| title_sort | novel duplex real time reverse transcription pcr assay for the detection of influenza a and the novel influenza a h1n1 strain |
| topic | influenza H1N1 real-time PCR detection |
| url | http://www.mdpi.com/1999-4915/1/3/1204/ |
| work_keys_str_mv | AT theopsloots anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT michaeldnissen anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT graemernimmo anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT stephenblambert anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT cassandraefaux anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT cherylbletchly anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT sewerynbialasiewicz anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT davidmwhiley anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT rebeccajrockett anovelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT theopsloots novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT michaeldnissen novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT graemernimmo novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT stephenblambert novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT cassandraefaux novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT cherylbletchly novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT sewerynbialasiewicz novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT davidmwhiley novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain AT rebeccajrockett novelduplexrealtimereversetranscriptionpcrassayforthedetectionofinfluenzaaandthenovelinfluenzaah1n1strain |