Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus
Background Bovine orthopneumovirus, formerly known as bovine respiratory syncytial virus (BRSV), is frequently associated with bovine respiratory disease (BRD). Aim To perform the molecular characterization of the G and F proteins of Brazilian wild-type BRSV strains derived from bovine respiratory i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Veterinary Quarterly |
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| Online Access: | http://dx.doi.org/10.1080/01652176.2020.1733704 |
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| author | Raquel Arruda Leme Alais Maria Dall Agnol Luciana Carvalho Balbo Fernanda Louise Pereira Flávia Possatti Alice Fernandes Alfieri Amauri Alcindo Alfieri |
| author_facet | Raquel Arruda Leme Alais Maria Dall Agnol Luciana Carvalho Balbo Fernanda Louise Pereira Flávia Possatti Alice Fernandes Alfieri Amauri Alcindo Alfieri |
| author_sort | Raquel Arruda Leme |
| collection | DOAJ |
| description | Background Bovine orthopneumovirus, formerly known as bovine respiratory syncytial virus (BRSV), is frequently associated with bovine respiratory disease (BRD). Aim To perform the molecular characterization of the G and F proteins of Brazilian wild-type BRSV strains derived from bovine respiratory infections in both beef and dairy cattle. Materials and Methods Ten BRSV strains derived from a dairy heifer rearing unit (n = 3) in 2011 and steers of three other feedlots (n = 7) in 2014 and 2015 were analyzed. For the BRSV G and F partial gene amplifications, RT-nested-PCR assays were performed with sequencing in both directions with forward and reverse primers used. Results The G gene-based analysis revealed that two strains were highly similar to the BRSV sequences representative of subgroup III, including the Bayovac vaccine strain. However, the remaining seven Brazilian BRSV strains were diverse when compared with strains representative of the BRSV I to VIII subgroups. The central hydrophobic region of the Brazilian BRSV G gene showed the replacement of conserved cysteines and other residues of importance to antibody reactivity. The deduced F gene amino acid sequences from the Brazilian BRSV strains showed changes that were absent in the representative sequences of the known subgroups. Viral isolation on the nasopharyngeal swab suspensions failed to isolate BRSV. Conclusion Results suggest that these strains represent a putative new subgroup of BRSV with mutations observed in the immunodominant region of the G protein. However, further studies on these Brazilian BRSV strains should be performed to establish their pathogenic potential. |
| first_indexed | 2024-12-14T11:41:50Z |
| format | Article |
| id | doaj.art-11a87aeb251946a5bffd7aa1e0dd1ddf |
| institution | Directory Open Access Journal |
| issn | 0165-2176 1875-5941 |
| language | English |
| last_indexed | 2024-12-14T11:41:50Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj.art-11a87aeb251946a5bffd7aa1e0dd1ddf2022-12-21T23:02:46ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412020-01-01401839610.1080/01652176.2020.17337041733704Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virusRaquel Arruda Leme0Alais Maria Dall Agnol1Luciana Carvalho Balbo2Fernanda Louise Pereira3Flávia Possatti4Alice Fernandes Alfieri5Amauri Alcindo Alfieri6National Institute of Science and Technology of Dairy Production Chain (INCT-Leite), Universidade Estadual de LondrinaNational Institute of Science and Technology of Dairy Production Chain (INCT-Leite), Universidade Estadual de LondrinaLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de LondrinaLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de LondrinaLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de LondrinaNational Institute of Science and Technology of Dairy Production Chain (INCT-Leite), Universidade Estadual de LondrinaNational Institute of Science and Technology of Dairy Production Chain (INCT-Leite), Universidade Estadual de LondrinaBackground Bovine orthopneumovirus, formerly known as bovine respiratory syncytial virus (BRSV), is frequently associated with bovine respiratory disease (BRD). Aim To perform the molecular characterization of the G and F proteins of Brazilian wild-type BRSV strains derived from bovine respiratory infections in both beef and dairy cattle. Materials and Methods Ten BRSV strains derived from a dairy heifer rearing unit (n = 3) in 2011 and steers of three other feedlots (n = 7) in 2014 and 2015 were analyzed. For the BRSV G and F partial gene amplifications, RT-nested-PCR assays were performed with sequencing in both directions with forward and reverse primers used. Results The G gene-based analysis revealed that two strains were highly similar to the BRSV sequences representative of subgroup III, including the Bayovac vaccine strain. However, the remaining seven Brazilian BRSV strains were diverse when compared with strains representative of the BRSV I to VIII subgroups. The central hydrophobic region of the Brazilian BRSV G gene showed the replacement of conserved cysteines and other residues of importance to antibody reactivity. The deduced F gene amino acid sequences from the Brazilian BRSV strains showed changes that were absent in the representative sequences of the known subgroups. Viral isolation on the nasopharyngeal swab suspensions failed to isolate BRSV. Conclusion Results suggest that these strains represent a putative new subgroup of BRSV with mutations observed in the immunodominant region of the G protein. However, further studies on these Brazilian BRSV strains should be performed to establish their pathogenic potential.http://dx.doi.org/10.1080/01652176.2020.1733704bovinebovine orthopneumovirusbovine respiratory diseasebrdbrsvcalf |
| spellingShingle | Raquel Arruda Leme Alais Maria Dall Agnol Luciana Carvalho Balbo Fernanda Louise Pereira Flávia Possatti Alice Fernandes Alfieri Amauri Alcindo Alfieri Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus Veterinary Quarterly bovine bovine orthopneumovirus bovine respiratory disease brd brsv calf |
| title | Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| title_full | Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| title_fullStr | Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| title_full_unstemmed | Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| title_short | Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| title_sort | molecular characterization of brazilian wild type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus |
| topic | bovine bovine orthopneumovirus bovine respiratory disease brd brsv calf |
| url | http://dx.doi.org/10.1080/01652176.2020.1733704 |
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