Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration
Objective: To investigate the transdifferentiation relationship between eight types of liver cell during rat liver regeneration (LR). Materials and Methods: 114 healthy Sprague-Dawley (SD) rats were used in this experimental study. Eight types of liver cell were isolated and purified with percoll...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2015-07-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/web/journal/article/406/download |
| _version_ | 1828389340385902592 |
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| author | Xiaguang Chen Cunshuan Xu |
| author_facet | Xiaguang Chen Cunshuan Xu |
| author_sort | Xiaguang Chen |
| collection | DOAJ |
| description | Objective: To investigate the transdifferentiation relationship between eight types of liver
cell during rat liver regeneration (LR).
Materials and Methods: 114 healthy Sprague-Dawley (SD) rats were used in this experimental
study. Eight types of liver cell were isolated and purified with percoll density
gradient centrifugation and immunomagentic bead methods. Marker genes for eight types
of cell were obtained by retrieving the relevant references and databases. Expression
changes of markers for each cell of the eight cell types were measured using microarray.
The relationships between the expression profiles of marker genes and transdifferentiation
among liver cells were analyzed using bioinformatics. Liver cell transdifferentiation
was predicted by comparing expression profiles of marker genes in different liver cells.
Results: During LR hepatocytes (HCs) not only express hepatic oval cells (HOC) markers
(including PROM1, KRT14 and LY6E), but also express biliary epithelial cell (BEC) markers
(including KRT7 and KRT19); BECs express both HOC markers (including GABRP,
PCNA and THY1) and HC markers such as CPS1, TAT, KRT8 and KRT18; both HC
markers (KRT18, KRT8 and WT1) and BEC markers (KRT7 and KRT19) were detected in
HOCs. Additionally, some HC markers were also significantly upregulated in hepatic stellate
cells ( HSCs), sinusoidal endothelial cells (SECs) , Kupffer cells (KCs) and dendritic
cells (DCs), mainly at 6-72 hours post partial hepatectomy (PH).
Conclusion: Our findings indicate that there is a mutual transdifferentiation relationship
between HC, BEC and HOC during LR, and a tendency for HSCs, SECs, KCs and DCs
to transdifferentiate into HCs. |
| first_indexed | 2024-12-10T06:27:38Z |
| format | Article |
| id | doaj.art-11aae3afb56d4a5c99a611d550e9f943 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T06:27:38Z |
| publishDate | 2015-07-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-11aae3afb56d4a5c99a611d550e9f9432022-12-22T01:59:09ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142015-07-01172339354Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver RegenerationXiaguang Chen0Cunshuan Xu1Animal Science and Technology School, Henan University of Science and Technology, Luoyang, ChinaKey Laboratory for Cell Differentiation Regulation, Henan Normal University, East of Construction Road, Xinxiang, ChinaObjective: To investigate the transdifferentiation relationship between eight types of liver cell during rat liver regeneration (LR). Materials and Methods: 114 healthy Sprague-Dawley (SD) rats were used in this experimental study. Eight types of liver cell were isolated and purified with percoll density gradient centrifugation and immunomagentic bead methods. Marker genes for eight types of cell were obtained by retrieving the relevant references and databases. Expression changes of markers for each cell of the eight cell types were measured using microarray. The relationships between the expression profiles of marker genes and transdifferentiation among liver cells were analyzed using bioinformatics. Liver cell transdifferentiation was predicted by comparing expression profiles of marker genes in different liver cells. Results: During LR hepatocytes (HCs) not only express hepatic oval cells (HOC) markers (including PROM1, KRT14 and LY6E), but also express biliary epithelial cell (BEC) markers (including KRT7 and KRT19); BECs express both HOC markers (including GABRP, PCNA and THY1) and HC markers such as CPS1, TAT, KRT8 and KRT18; both HC markers (KRT18, KRT8 and WT1) and BEC markers (KRT7 and KRT19) were detected in HOCs. Additionally, some HC markers were also significantly upregulated in hepatic stellate cells ( HSCs), sinusoidal endothelial cells (SECs) , Kupffer cells (KCs) and dendritic cells (DCs), mainly at 6-72 hours post partial hepatectomy (PH). Conclusion: Our findings indicate that there is a mutual transdifferentiation relationship between HC, BEC and HOC during LR, and a tendency for HSCs, SECs, KCs and DCs to transdifferentiate into HCs.http://celljournal.org/web/journal/article/406/downloadCell TransdifferentiationRat Liver RegenerationCell Isolation |
| spellingShingle | Xiaguang Chen Cunshuan Xu Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration Cell Journal Cell Transdifferentiation Rat Liver Regeneration Cell Isolation |
| title | Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration |
| title_full | Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration |
| title_fullStr | Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration |
| title_full_unstemmed | Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration |
| title_short | Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration |
| title_sort | transcription profiles of marker genes predict the transdifferentiation relationship between eight types of liver cell during rat liver regeneration |
| topic | Cell Transdifferentiation Rat Liver Regeneration Cell Isolation |
| url | http://celljournal.org/web/journal/article/406/download |
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