Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">Th...

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Main Authors: Ririn Nurul Fauziah, Dinamella Wahjuningrum, , Sukenda, , Ranta
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2015-10-01
Series:Jurnal Akuakultur Indonesia
Online Access:http://journal.ipb.ac.id/index.php/jai/article/view/10340
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author Ririn Nurul Fauziah
Dinamella Wahjuningrum
, Sukenda
, Ranta
author_facet Ririn Nurul Fauziah
Dinamella Wahjuningrum
, Sukenda
, Ranta
author_sort Ririn Nurul Fauziah
collection DOAJ
description <p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">This study was aimed at determining potential of combination powder of garlic <em>Allium sativum</em>-shatterstone herb <em>Phyllanthus niruri</em> supplemented in feed against <em>S. agalactiae</em> infection in tilapia. Four concentrations of combination powder of <em>A. sativum</em>-<em>P. Niruri</em>; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in <em>in vitro</em> test. <em>In vivo</em> test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed (BM).  The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL <em>S. agalactiae</em> at concentration of 10<sup>5</sup> cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P&lt;0.05. In addition, <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed was also able to enhance the immune response by increasing phagocytic activity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Streptococcus agalactiae</em>, phytopharmacy, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, tilapia</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih <em>Allium sativum</em>-meniran <em>Phyllanthus niruri </em>dalam pakan terhadap pencegahan infeksi bakteri <em>S. agalactiae</em> pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam  menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji <em>in vitro</em>. Uji <em>in vivo</em> terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri <em>S. agalactiae</em> sebanyak 0,1 mL dengan kepadatan 10<sup>5 </sup>cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P&lt;0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Streptococcus agalactiae</em>, fitofarmaka, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, ikan nila</p><p> </p>
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spelling doaj.art-11aafa11ae3b471e842cfcdabdbb5cbc2022-12-22T01:04:13ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-10-01141798910.19027/jai.14.79-898514Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapiaRirin Nurul Fauziah0Dinamella Wahjuningrum1, Sukenda2, Ranta3Departemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian Bogor<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">This study was aimed at determining potential of combination powder of garlic <em>Allium sativum</em>-shatterstone herb <em>Phyllanthus niruri</em> supplemented in feed against <em>S. agalactiae</em> infection in tilapia. Four concentrations of combination powder of <em>A. sativum</em>-<em>P. Niruri</em>; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in <em>in vitro</em> test. <em>In vivo</em> test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed (BM).  The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL <em>S. agalactiae</em> at concentration of 10<sup>5</sup> cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P&lt;0.05. In addition, <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed was also able to enhance the immune response by increasing phagocytic activity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Streptococcus agalactiae</em>, phytopharmacy, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, tilapia</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih <em>Allium sativum</em>-meniran <em>Phyllanthus niruri </em>dalam pakan terhadap pencegahan infeksi bakteri <em>S. agalactiae</em> pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam  menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji <em>in vitro</em>. Uji <em>in vivo</em> terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri <em>S. agalactiae</em> sebanyak 0,1 mL dengan kepadatan 10<sup>5 </sup>cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P&lt;0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Streptococcus agalactiae</em>, fitofarmaka, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, ikan nila</p><p> </p>http://journal.ipb.ac.id/index.php/jai/article/view/10340
spellingShingle Ririn Nurul Fauziah
Dinamella Wahjuningrum
, Sukenda
, Ranta
Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
Jurnal Akuakultur Indonesia
title Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
title_full Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
title_fullStr Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
title_full_unstemmed Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
title_short Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia
title_sort combination of garlic shatterstone herb powder to control streptococcus agalactiae infection in tilapia
url http://journal.ipb.ac.id/index.php/jai/article/view/10340
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AT dinamellawahjuningrum combinationofgarlicshatterstoneherbpowdertocontrolstreptococcusagalactiaeinfectionintilapia
AT sukenda combinationofgarlicshatterstoneherbpowdertocontrolstreptococcusagalactiaeinfectionintilapia
AT ranta combinationofgarlicshatterstoneherbpowdertocontrolstreptococcusagalactiaeinfectionintilapia