A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subti...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2023-01-01
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| Series: | Journal of the Serbian Chemical Society |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0352-5139/2023/0352-51392300011S.pdf |
| _version_ | 1827586793070919680 |
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| author | Šokarda-Slavić Marinela Ralić Vanja Nastasijević Branislav Matijević Milica Vujčić Zoran Margetić Aleksandra |
| author_facet | Šokarda-Slavić Marinela Ralić Vanja Nastasijević Branislav Matijević Milica Vujčić Zoran Margetić Aleksandra |
| author_sort | Šokarda-Slavić Marinela |
| collection | DOAJ |
| description | Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box–Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS- -PAGE followed by methylene blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. It is encouraging that PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone. |
| first_indexed | 2024-03-09T00:06:52Z |
| format | Article |
| id | doaj.art-11b2cbd4170a40e78ae7bc726f5759d5 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-03-09T00:06:52Z |
| publishDate | 2023-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-11b2cbd4170a40e78ae7bc726f5759d52023-12-12T13:08:14ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212023-01-01881098599710.2298/JSC221116011S0352-51392300011SA novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strainŠokarda-Slavić Marinela0https://orcid.org/0000-0002-9041-552XRalić Vanja1https://orcid.org/0000-0002-3206-1831Nastasijević Branislav2https://orcid.org/0000-0002-4529-1599Matijević Milica3https://orcid.org/0000-0002-9846-6671Vujčić Zoran4https://orcid.org/0000-0002-8963-2439Margetić Aleksandra5https://orcid.org/0000-0003-2445-0375University of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Department of Chemistry, Belgrade, Serbia Center for light-based research and technologies, COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaCenter for light-based research and technologies, COHERENCE, Department of Atomic Physics, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia University of Belgrade, Faculty of Chemistry, Department of Biochemistry, Belgrade, SerbiaUniversity of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Department of Chemistry, Belgrade, SerbiaPoly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box–Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS- -PAGE followed by methylene blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. It is encouraging that PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.https://doiserbia.nb.rs/img/doi/0352-5139/2023/0352-51392300011S.pdfpga productionwild-type bacillus strainpga characterizationcytotoxicity |
| spellingShingle | Šokarda-Slavić Marinela Ralić Vanja Nastasijević Branislav Matijević Milica Vujčić Zoran Margetić Aleksandra A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain Journal of the Serbian Chemical Society pga production wild-type bacillus strain pga characterization cytotoxicity |
| title | A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain |
| title_full | A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain |
| title_fullStr | A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain |
| title_full_unstemmed | A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain |
| title_short | A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain |
| title_sort | novel pga tio2 nanocomposite prepared with poly γ glutamic acid from the newly isolated bacillus subtilis 17b strain |
| topic | pga production wild-type bacillus strain pga characterization cytotoxicity |
| url | https://doiserbia.nb.rs/img/doi/0352-5139/2023/0352-51392300011S.pdf |
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