Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering
Extracelllar matrix (ECM) materials from mammalian tissues have been broadly used for tissue engineering for animal models. While decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. In This study we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2012-09-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_26113_b60f5b4ade02bcc91e4b848cd3617250.pdf |
| _version_ | 1818060803733979136 |
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| author | Amin Tavassoli Nasser Mahdavi-Shahri Maryam M. Matin Masoud Fereidoni Fahimeh Shahabipour |
| author_facet | Amin Tavassoli Nasser Mahdavi-Shahri Maryam M. Matin Masoud Fereidoni Fahimeh Shahabipour |
| author_sort | Amin Tavassoli |
| collection | DOAJ |
| description | Extracelllar matrix (ECM) materials from mammalian tissues have been broadly used for tissue engineering for animal models. While decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. In This study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construct for cartilage substitution. For this purpose, different decellularization protocols including freeze/thaw cycles followed by treatment with various concentrations of sodium dodecyl sulfate (SDS) were used. Decellularization was analyzed by histological examination including hematoxylin and eosin and picrosirius staining. Treatment with 2%SDS for 5 to 8h followed by 4% SDS for3h hada significant effect on decellularization process with the minimal effect on the collagen contents. On the other hand, Treatment with 1% SDS had no effect on cell removal and use of 8% SDS for 5 to 8 h resulted in complete elimination of cells and significant decrease in cartilage matrix and collagen contents. This study provides a technique to produce acellular ECM derived from articular bovine cartilage whichmay serve as a xenogenicscaffold for cartilage tissue engineering. |
| first_indexed | 2024-12-10T13:38:14Z |
| format | Article |
| id | doaj.art-11b5a061966d461c8bd62c6ef3619320 |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-12-10T13:38:14Z |
| publishDate | 2012-09-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-11b5a061966d461c8bd62c6ef36193202022-12-22T01:46:46ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062012-09-01411810.22067/veterinary.v4i1.1787826113Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineeringAmin Tavassoli0Nasser Mahdavi-Shahri1Maryam M. Matin2Masoud Fereidoni3Fahimeh Shahabipour4Ferdowsi University of MashhadFerdowsi University of MashhadFerdowsi University of MashhadFerdowsi University of MashhadFerdowsi University of MashhadExtracelllar matrix (ECM) materials from mammalian tissues have been broadly used for tissue engineering for animal models. While decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. In This study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construct for cartilage substitution. For this purpose, different decellularization protocols including freeze/thaw cycles followed by treatment with various concentrations of sodium dodecyl sulfate (SDS) were used. Decellularization was analyzed by histological examination including hematoxylin and eosin and picrosirius staining. Treatment with 2%SDS for 5 to 8h followed by 4% SDS for3h hada significant effect on decellularization process with the minimal effect on the collagen contents. On the other hand, Treatment with 1% SDS had no effect on cell removal and use of 8% SDS for 5 to 8 h resulted in complete elimination of cells and significant decrease in cartilage matrix and collagen contents. This study provides a technique to produce acellular ECM derived from articular bovine cartilage whichmay serve as a xenogenicscaffold for cartilage tissue engineering.https://ijvst.um.ac.ir/article_26113_b60f5b4ade02bcc91e4b848cd3617250.pdfbovine articular cartilagedecellularized matrixsodium dodecyl sulfatetissue engineering |
| spellingShingle | Amin Tavassoli Nasser Mahdavi-Shahri Maryam M. Matin Masoud Fereidoni Fahimeh Shahabipour Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering The Iranian Journal of Veterinary Science and Technology bovine articular cartilage decellularized matrix sodium dodecyl sulfate tissue engineering |
| title | Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| title_full | Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| title_fullStr | Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| title_full_unstemmed | Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| title_short | Bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| title_sort | bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering |
| topic | bovine articular cartilage decellularized matrix sodium dodecyl sulfate tissue engineering |
| url | https://ijvst.um.ac.ir/article_26113_b60f5b4ade02bcc91e4b848cd3617250.pdf |
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