Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K
Lactic acid bacteria (LAB) resist sodium selenite of concentrations greater than 100 mg/L in fermentation media. Selenium affects the growth rate, but once the microorganism absorbs selenium, this element is converted through a complex mechanism into selenocysteine and then into a selenoprotein stru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/7/684 |
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| author | Meyli Claudia Escobar-Ramírez Gabriela Mariana Rodríguez-Serrano Eduardo Zúñiga-León Mario Adolfo García-Montes Emmanuel Pérez-Escalante Luis Guillermo González-Olivares |
| author_facet | Meyli Claudia Escobar-Ramírez Gabriela Mariana Rodríguez-Serrano Eduardo Zúñiga-León Mario Adolfo García-Montes Emmanuel Pérez-Escalante Luis Guillermo González-Olivares |
| author_sort | Meyli Claudia Escobar-Ramírez |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) resist sodium selenite of concentrations greater than 100 mg/L in fermentation media. Selenium affects the growth rate, but once the microorganism absorbs selenium, this element is converted through a complex mechanism into selenocysteine and then into a selenoprotein structure. This study verified the presence of selenocysteine in <i>Enterococcus faecium</i> ABMC-05. The microorganism was cultivated in a medium enriched with a minimum inhibitory concentration of sodium selenite (184 mg/L). The concentration of selenium absorbed and the bioconversion into selenocysteine were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) and reverse-phase high-performance chromatography (RP-HPLC), respectively. The presence of the <i>sel</i>D, <i>sel</i>A, and <i>cys</i>K genes was determined by amplifying the 16S rDNA through polymerase chain reaction (PCR). The microorganism accumulated inorganic selenium, and part was transformed into selenocysteine. The growth curves were atypical for a lactic acid bacterium with a stationary phase greater than 70 h. Determining the genetic expression showed only the presence of the <i>cys</i>K gene and the absence of the <i>sel</i>D and the <i>sel</i>A genes. The results demonstrate that this microorganism produces selenocysteine through a mechanism independent of the SelA and SelD pathways in contrast to other LAB. |
| first_indexed | 2024-03-11T01:05:22Z |
| format | Article |
| id | doaj.art-11b6cf7a42584014a9428cf73d5792a3 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:05:22Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-11b6cf7a42584014a9428cf73d5792a32023-11-18T19:16:58ZengMDPI AGFermentation2311-56372023-07-019768410.3390/fermentation9070684Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>KMeyli Claudia Escobar-Ramírez0Gabriela Mariana Rodríguez-Serrano1Eduardo Zúñiga-León2Mario Adolfo García-Montes3Emmanuel Pérez-Escalante4Luis Guillermo González-Olivares5Centro Nacional de Investigacion Disciplinaria en Fisiología y Mejoramiento Animal, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Ajuchitlán, Colón, Querétaro 76280, MexicoDepartamento de Biotecnología, División de Ciencias Biológicas y de la Salud, Unidad Iztapalapa, Universidad Autónoma Metropolitana, Ciudad de México 09340, MexicoCentro de Investigaciones en Recursos Bióticos, Facultad de Ciencias, Universidad Autónoma del Estado de México, Carretera Toluca-Ixtlahuaca km 14.5, San Cayetano, Toluca 50295, MexicoCentro de Investigaciones Biológicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km. 4.5, Pachuca 42067, MexicoCentro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km. 4.5, Pachuca 42067, MexicoCentro de Investigaciones Químicas, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo km. 4.5, Pachuca 42067, MexicoLactic acid bacteria (LAB) resist sodium selenite of concentrations greater than 100 mg/L in fermentation media. Selenium affects the growth rate, but once the microorganism absorbs selenium, this element is converted through a complex mechanism into selenocysteine and then into a selenoprotein structure. This study verified the presence of selenocysteine in <i>Enterococcus faecium</i> ABMC-05. The microorganism was cultivated in a medium enriched with a minimum inhibitory concentration of sodium selenite (184 mg/L). The concentration of selenium absorbed and the bioconversion into selenocysteine were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) and reverse-phase high-performance chromatography (RP-HPLC), respectively. The presence of the <i>sel</i>D, <i>sel</i>A, and <i>cys</i>K genes was determined by amplifying the 16S rDNA through polymerase chain reaction (PCR). The microorganism accumulated inorganic selenium, and part was transformed into selenocysteine. The growth curves were atypical for a lactic acid bacterium with a stationary phase greater than 70 h. Determining the genetic expression showed only the presence of the <i>cys</i>K gene and the absence of the <i>sel</i>D and the <i>sel</i>A genes. The results demonstrate that this microorganism produces selenocysteine through a mechanism independent of the SelA and SelD pathways in contrast to other LAB.https://www.mdpi.com/2311-5637/9/7/684selenocysteineseleniumlactic acid bacteriaselenoproteins |
| spellingShingle | Meyli Claudia Escobar-Ramírez Gabriela Mariana Rodríguez-Serrano Eduardo Zúñiga-León Mario Adolfo García-Montes Emmanuel Pérez-Escalante Luis Guillermo González-Olivares Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K Fermentation selenocysteine selenium lactic acid bacteria selenoproteins |
| title | Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K |
| title_full | Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K |
| title_fullStr | Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K |
| title_full_unstemmed | Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K |
| title_short | Selenocysteine Formation by <i>Enterococcus faecium</i> ABMC-05 Follows a Mechanism That Is Not Dependent on Genes <i>sel</i>A and <i>sel</i>D but on Gene <i>cys</i>K |
| title_sort | selenocysteine formation by i enterococcus faecium i abmc 05 follows a mechanism that is not dependent on genes i sel i a and i sel i d but on gene i cys i k |
| topic | selenocysteine selenium lactic acid bacteria selenoproteins |
| url | https://www.mdpi.com/2311-5637/9/7/684 |
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