Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes
In this study, we evaluated the effectiveness of using estrogen-induced prolonged luteal function followed by prostaglandin F2 alpha (PGF2α) treatment to synchronize estrus in gilts. On day12 of the estrus cycle (D0 = first day of standing estrus), 52 gilts were assigned at random to two experimenta...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/23/3393 |
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| author | Diego Feitosa Leal Carlos Henrique Cabral Viana Glen William Almond Matheus Saliba Monteiro Cesar Augusto Pospissil Garbossa Rafaella Fernandes Carnevale Bruno Bracco Donatelli Muro André Pegoraro Poor Guilherme Pugliesi Marcílio Nichi Tatiane Terumi Negrão Watanabe Mariana Groke Marques |
| author_facet | Diego Feitosa Leal Carlos Henrique Cabral Viana Glen William Almond Matheus Saliba Monteiro Cesar Augusto Pospissil Garbossa Rafaella Fernandes Carnevale Bruno Bracco Donatelli Muro André Pegoraro Poor Guilherme Pugliesi Marcílio Nichi Tatiane Terumi Negrão Watanabe Mariana Groke Marques |
| author_sort | Diego Feitosa Leal |
| collection | DOAJ |
| description | In this study, we evaluated the effectiveness of using estrogen-induced prolonged luteal function followed by prostaglandin F2 alpha (PGF2α) treatment to synchronize estrus in gilts. On day12 of the estrus cycle (D0 = first day of standing estrus), 52 gilts were assigned at random to two experimental groups: non-treated gilts (CON, <i>n</i> = 22), serving as controls, and prolonged luteal function group (CYP, <i>n</i> = 30), receiving a single treatment with 10 mg of estradiol cypionate intramuscularly Starting on day 12, blood samples were collected for estradiol and progesterone assays. Estrus detection started on day 17. Gilts from the CON group were inseminated at the onset of natural estrus. On day 28 CYP gilts were treated with PGF2α to induce luteolysis and inseminated at the onset of estrus. Gilts were slaughtered 5 d after the last insemination. A single treatment with estradiol cypionate prolonged luteal function in 90% of treated gilts. The duration of the estrous cycle was longer (<i>p</i> < 0.0001) for CYP gilts compared to CON gilts. CYP gilts showed synchronized estrus 3.96 ± 0.19 d after induction of luteolysis. The conception rate was similar (<i>p</i> = 0.10) for CON and CYP gilts. No difference was observed in the embryo recovery rate (<i>p</i> = 0.18) and total number of embryos per female (<i>p</i> = 0.06). The percentage of unfertilized oocytes, fragmented embryos and viable embryos was similar among females from CON and CYP groups (<i>p</i> > 0.05). The treatment of gilts with a single application of 10 mg of estradiol cypionate on day 12 of the estrous cycle was effective in prolonging luteal function and treatment with PGF2α resulted in synchronized estrus. Additionally, the synchronization protocol had no deleterious effect on fertility and embryonic development. |
| first_indexed | 2024-03-09T17:54:54Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T17:54:54Z |
| publishDate | 2022-12-01 |
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| series | Animals |
| spelling | doaj.art-11b6d970beba43768925df9d12e3c1562023-11-24T10:26:24ZengMDPI AGAnimals2076-26152022-12-011223339310.3390/ani12233393Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive OutcomesDiego Feitosa Leal0Carlos Henrique Cabral Viana1Glen William Almond2Matheus Saliba Monteiro3Cesar Augusto Pospissil Garbossa4Rafaella Fernandes Carnevale5Bruno Bracco Donatelli Muro6André Pegoraro Poor7Guilherme Pugliesi8Marcílio Nichi9Tatiane Terumi Negrão Watanabe10Mariana Groke Marques11Department of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilSchool of Veterinary Medicine, Pontifical Catholic University Minas, Poços de Caldas 37714-620, BrazilDepartment of Population Health & Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU), Raleigh, NC 27607, USADepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), São Paulo 05508-270, BrazilDepartment of Nutrition and Animal Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilDepartment of Nutrition and Animal Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilDepartment of Nutrition and Animal Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), São Paulo 05508-270, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilDepartment of Animal Reproduction, School of Veterinary Medicine and Animal Sciences, University of São Paulo (USP), Pirassununga 13635-900, BrazilDepartment of Population Health & Pathobiology, College of Veterinary Medicine, North Carolina State University (NCSU), Raleigh, NC 27607, USAEmbrapa Suínos e Aves, Concórdia 89715-899, BrazilIn this study, we evaluated the effectiveness of using estrogen-induced prolonged luteal function followed by prostaglandin F2 alpha (PGF2α) treatment to synchronize estrus in gilts. On day12 of the estrus cycle (D0 = first day of standing estrus), 52 gilts were assigned at random to two experimental groups: non-treated gilts (CON, <i>n</i> = 22), serving as controls, and prolonged luteal function group (CYP, <i>n</i> = 30), receiving a single treatment with 10 mg of estradiol cypionate intramuscularly Starting on day 12, blood samples were collected for estradiol and progesterone assays. Estrus detection started on day 17. Gilts from the CON group were inseminated at the onset of natural estrus. On day 28 CYP gilts were treated with PGF2α to induce luteolysis and inseminated at the onset of estrus. Gilts were slaughtered 5 d after the last insemination. A single treatment with estradiol cypionate prolonged luteal function in 90% of treated gilts. The duration of the estrous cycle was longer (<i>p</i> < 0.0001) for CYP gilts compared to CON gilts. CYP gilts showed synchronized estrus 3.96 ± 0.19 d after induction of luteolysis. The conception rate was similar (<i>p</i> = 0.10) for CON and CYP gilts. No difference was observed in the embryo recovery rate (<i>p</i> = 0.18) and total number of embryos per female (<i>p</i> = 0.06). The percentage of unfertilized oocytes, fragmented embryos and viable embryos was similar among females from CON and CYP groups (<i>p</i> > 0.05). The treatment of gilts with a single application of 10 mg of estradiol cypionate on day 12 of the estrous cycle was effective in prolonging luteal function and treatment with PGF2α resulted in synchronized estrus. Additionally, the synchronization protocol had no deleterious effect on fertility and embryonic development.https://www.mdpi.com/2076-2615/12/23/3393estradiol cypionateprostaglandinestrous cyclesynchronizationfertilityembryo development |
| spellingShingle | Diego Feitosa Leal Carlos Henrique Cabral Viana Glen William Almond Matheus Saliba Monteiro Cesar Augusto Pospissil Garbossa Rafaella Fernandes Carnevale Bruno Bracco Donatelli Muro André Pegoraro Poor Guilherme Pugliesi Marcílio Nichi Tatiane Terumi Negrão Watanabe Mariana Groke Marques Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes Animals estradiol cypionate prostaglandin estrous cycle synchronization fertility embryo development |
| title | Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes |
| title_full | Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes |
| title_fullStr | Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes |
| title_full_unstemmed | Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes |
| title_short | Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF<sub>2α</sub> and Its Effects on Reproductive Outcomes |
| title_sort | estrus synchronization of replacement gilts using estradiol cipionate and pgf sub 2α sub and its effects on reproductive outcomes |
| topic | estradiol cypionate prostaglandin estrous cycle synchronization fertility embryo development |
| url | https://www.mdpi.com/2076-2615/12/23/3393 |
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