Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2
Introduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2018-06-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/3864 |
| _version_ | 1828518391989665792 |
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| author | Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol |
| author_facet | Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol |
| author_sort | Pierina D’Angelo |
| collection | DOAJ |
| description | Introduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prevalent subtype 2j.
Objective: To evaluate the performance of methodologies for genotyping HCV, particularly for identification of subtype 2j.
Materials and methods: HCV genotype and subtype were determined by reverse hybridization technique (LiPA) and sequencing of the HCV 5’UTR and NS5B regions.
Results: A total of 65 samples from HCV-infected patients were analyzed. PCR amplifications of the 5’UTR region exhibited the highest sensitivity (100% vs 91% for LiPA and 77% for NS5B). Genotype determination, taking as reference test NS5B, showed 100% concordance with the other methods, and 67% and 59% for subtypes with 5´NC and LiPA, respectively. NS5B sequencing allowed the identification of subtypes 2j and 2s, which were not detected by the other methods. A specific LiPA pattern was not
observed for HCV subtype 2j.
Conclusion: Although being the methodology with lowest sensitivity for amplification of HCV RNA, sequencing NS5B region remains a powerful tool for correct discrimination of the different HCV subtypes, which is of epidemiological relevance. |
| first_indexed | 2024-12-11T18:54:43Z |
| format | Article |
| id | doaj.art-11b8188ed6d7466fb1ac80caacf79255 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-12-11T18:54:43Z |
| publishDate | 2018-06-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-11b8188ed6d7466fb1ac80caacf792552022-12-22T00:54:10ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572018-06-0138228228810.7705/biomedica.v38i0.38642108Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2Pierina D’Angelo0Rossana Celeste Jaspe1Carmen Luisa Loureiro2Cristina Gutiérrez3María Zulay Sulbarán4Yoneira Sulbarán5Felix Toro6Flor Helene Pujol7Laboratorio de Programas Especiales-Hepatitis y SIDA, Instituto Nacional de Higiene “Rafael Rangel”, Caracas, VenezuelaLaboratorio de Virología Molecular, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaLaboratorio de Virología Molecular, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaLaboratorio de Programas Especiales-Hepatitis y SIDA, Instituto Nacional de Higiene “Rafael Rangel”, Caracas, VenezuelaLaboratorio de Virología, Postgrado en Biología Aplicada, Universidad de Oriente, Núcleo Sucre, Cumaná VenezuelaLaboratorio de Virología Molecular, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaInstituto de Inmunología, Universidad Central de Venezuela, Caracas, VenezuelaLaboratorio de Virología Molecular, Instituto Venezolano de Investigaciones Científicas, Caracas, VenezuelaIntroduction: Hepatitis C virus (HCV) displays high genetic variability, with seven genotypes and numerous subtypes. The determination of the viral type has been essential for the selection and timing of antiviral treatment. In Venezuela, HCV genotype 2 is relatively diverse, being particularly prevalent subtype 2j. Objective: To evaluate the performance of methodologies for genotyping HCV, particularly for identification of subtype 2j. Materials and methods: HCV genotype and subtype were determined by reverse hybridization technique (LiPA) and sequencing of the HCV 5’UTR and NS5B regions. Results: A total of 65 samples from HCV-infected patients were analyzed. PCR amplifications of the 5’UTR region exhibited the highest sensitivity (100% vs 91% for LiPA and 77% for NS5B). Genotype determination, taking as reference test NS5B, showed 100% concordance with the other methods, and 67% and 59% for subtypes with 5´NC and LiPA, respectively. NS5B sequencing allowed the identification of subtypes 2j and 2s, which were not detected by the other methods. A specific LiPA pattern was not observed for HCV subtype 2j. Conclusion: Although being the methodology with lowest sensitivity for amplification of HCV RNA, sequencing NS5B region remains a powerful tool for correct discrimination of the different HCV subtypes, which is of epidemiological relevance.https://www.revistabiomedica.org/index.php/biomedica/article/view/3864 |
| spellingShingle | Pierina D’Angelo Rossana Celeste Jaspe Carmen Luisa Loureiro Cristina Gutiérrez María Zulay Sulbarán Yoneira Sulbarán Felix Toro Flor Helene Pujol Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 Biomédica: revista del Instituto Nacional de Salud |
| title | Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
| title_full | Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
| title_fullStr | Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
| title_full_unstemmed | Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
| title_short | Performance of molecular methods for identification of unusual subtypes of hepatitis C virus genotype 2 |
| title_sort | performance of molecular methods for identification of unusual subtypes of hepatitis c virus genotype 2 |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/3864 |
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