Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation
<i>Dunaliella salina</i> (Chlorophyceae), <i>Phormidium versicolor</i> (Cyanophyceae), and <i>Cylindrotheca closterium</i> (Bacillariophyceae) were isolated from three ponds in the solar saltern of Sfax (Tunisia). Growth, pigment contents, and photosynthetic and a...
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2023-01-01
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| author | Wassim Guermazi Salma Masmoudi Neila Annabi Trabelsi Sana Gammoudi Habib Ayadi Annick Morant-Manceau George N. Hotos |
| author_facet | Wassim Guermazi Salma Masmoudi Neila Annabi Trabelsi Sana Gammoudi Habib Ayadi Annick Morant-Manceau George N. Hotos |
| author_sort | Wassim Guermazi |
| collection | DOAJ |
| description | <i>Dunaliella salina</i> (Chlorophyceae), <i>Phormidium versicolor</i> (Cyanophyceae), and <i>Cylindrotheca closterium</i> (Bacillariophyceae) were isolated from three ponds in the solar saltern of Sfax (Tunisia). Growth, pigment contents, and photosynthetic and antioxidant enzyme activities were measured under controlled conditions of three light levels (300, 500, and 1000 µmol photons m<sup>−2</sup> s<sup>−1</sup>) and three NaCl concentrations (40, 80, and 140 g L<sup>−1</sup>). The highest salinity reduced the growth of <i>D. salina</i> and <i>P. versicolor</i> NCC466 and strongly inhibited that of <i>C. closterium</i>. According to ΦPSII values, the photosynthetic apparatus of <i>P. versicolor</i> was stimulated by increasing salinity, whereas that of <i>D. salina</i> and <i>C. closterium</i> was decreased by irradiance rise. The production of carotenoids in <i>D. salina</i> and <i>P. versicolor</i> was stimulated when salinity and irradiance increased, whereas it decreased in the diatom. Catalase (CAT), Superoxide dismutase (SOD), and Ascorbate peroxidase (APX) activities were only detected when the three species were cultivated under E1000. The antioxidant activity of carotenoids could compensate for the low antioxidant enzyme activity measured in <i>D. salina</i>. Salinity and irradiation levels interact with the physiology of three species that have mechanisms of more or less effective stress resistance, hence different resistance to environmental stresses according to the species. Under these stress-controlled conditions, <i>P. versicolor</i> and <i>C. closterium</i> strains could provide promising sources of extremolyte for several purposes. |
| first_indexed | 2024-03-11T08:33:12Z |
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| language | English |
| last_indexed | 2024-03-11T08:33:12Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-11b8289f40124ecc8bc2ccc9cb9400af2023-11-16T21:39:33ZengMDPI AGLife2075-17292023-01-0113231310.3390/life13020313Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and IrradiationWassim Guermazi0Salma Masmoudi1Neila Annabi Trabelsi2Sana Gammoudi3Habib Ayadi4Annick Morant-Manceau5George N. Hotos6Laboratory of Marine Biodiversity and Environment (LR18ES/30), University of Sfax, Sfax CP 3000, TunisiaLaboratory of Marine Biodiversity and Environment (LR18ES/30), University of Sfax, Sfax CP 3000, TunisiaLaboratory of Marine Biodiversity and Environment (LR18ES/30), University of Sfax, Sfax CP 3000, TunisiaLaboratory of Marine Biodiversity and Environment (LR18ES/30), University of Sfax, Sfax CP 3000, TunisiaLaboratory of Marine Biodiversity and Environment (LR18ES/30), University of Sfax, Sfax CP 3000, TunisiaLUNAM, Laboratoire Mer, Molécules, Santé (EA 2160), Université du Maine, Avenue Olivier Messiaen, CEDEX 9, 72085 Le Mans, FrancePlankton Culture Laboratory, Department of Fisheries and Aquaculture, University of Patras, 30200 Messolonghi, Greece<i>Dunaliella salina</i> (Chlorophyceae), <i>Phormidium versicolor</i> (Cyanophyceae), and <i>Cylindrotheca closterium</i> (Bacillariophyceae) were isolated from three ponds in the solar saltern of Sfax (Tunisia). Growth, pigment contents, and photosynthetic and antioxidant enzyme activities were measured under controlled conditions of three light levels (300, 500, and 1000 µmol photons m<sup>−2</sup> s<sup>−1</sup>) and three NaCl concentrations (40, 80, and 140 g L<sup>−1</sup>). The highest salinity reduced the growth of <i>D. salina</i> and <i>P. versicolor</i> NCC466 and strongly inhibited that of <i>C. closterium</i>. According to ΦPSII values, the photosynthetic apparatus of <i>P. versicolor</i> was stimulated by increasing salinity, whereas that of <i>D. salina</i> and <i>C. closterium</i> was decreased by irradiance rise. The production of carotenoids in <i>D. salina</i> and <i>P. versicolor</i> was stimulated when salinity and irradiance increased, whereas it decreased in the diatom. Catalase (CAT), Superoxide dismutase (SOD), and Ascorbate peroxidase (APX) activities were only detected when the three species were cultivated under E1000. The antioxidant activity of carotenoids could compensate for the low antioxidant enzyme activity measured in <i>D. salina</i>. Salinity and irradiation levels interact with the physiology of three species that have mechanisms of more or less effective stress resistance, hence different resistance to environmental stresses according to the species. Under these stress-controlled conditions, <i>P. versicolor</i> and <i>C. closterium</i> strains could provide promising sources of extremolyte for several purposes.https://www.mdpi.com/2075-1729/13/2/313light-salt stressmicroalgae<i>Dunaliella salina</i><i>Cylindrotheca closterium</i><i>Phormidium versicolor</i>PSII |
| spellingShingle | Wassim Guermazi Salma Masmoudi Neila Annabi Trabelsi Sana Gammoudi Habib Ayadi Annick Morant-Manceau George N. Hotos Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation Life light-salt stress microalgae <i>Dunaliella salina</i> <i>Cylindrotheca closterium</i> <i>Phormidium versicolor</i> PSII |
| title | Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation |
| title_full | Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation |
| title_fullStr | Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation |
| title_full_unstemmed | Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation |
| title_short | Physiological and Biochemical Responses in Microalgae <i>Dunaliella salina</i>, <i>Cylindrotheca closterium</i> and <i>Phormidium versicolor</i> NCC466 Exposed to High Salinity and Irradiation |
| title_sort | physiological and biochemical responses in microalgae i dunaliella salina i i cylindrotheca closterium i and i phormidium versicolor i ncc466 exposed to high salinity and irradiation |
| topic | light-salt stress microalgae <i>Dunaliella salina</i> <i>Cylindrotheca closterium</i> <i>Phormidium versicolor</i> PSII |
| url | https://www.mdpi.com/2075-1729/13/2/313 |
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