Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity
Abstract Background/aims Sheep are important livestock with variant ovulation rate and fertility. Dorset sheep is a typical breed with low prolificacy, whereas Small Tail Han sheep with FecB mutation (HanBB) have hyperprolificacy. Our previous studies have revealed the gene expression difference bet...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-020-00633-9 |
| _version_ | 1819319763562659840 |
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| author | Xiangyang Miao Qingmiao Luo Lingli Xie Huijing Zhao Xiaoyu Qin |
| author_facet | Xiangyang Miao Qingmiao Luo Lingli Xie Huijing Zhao Xiaoyu Qin |
| author_sort | Xiangyang Miao |
| collection | DOAJ |
| description | Abstract Background/aims Sheep are important livestock with variant ovulation rate and fertility. Dorset sheep is a typical breed with low prolificacy, whereas Small Tail Han sheep with FecB mutation (HanBB) have hyperprolificacy. Our previous studies have revealed the gene expression difference between the ovaries from Dorset and HanBB sheep contributes to the difference of fecundity, however, what leads to these gene expression difference remains unclear. DNA methylation, an important epigenetic process, plays a crucial role in gene expression regulation. Methods In the present study, we constructed a methylated DNA immunoprecipitation combined with high throughput sequencing (MeDIP-seq) strategy to investigate the differentially methylated genes between the Dorset and HanBB ovaries. Results Our findings suggest the genes involved in immune response, branched-chain amino acid metabolism, cell growth and cell junction were differentially methylated in or around the gene body regions. Conclusions These findings provide prospective insights on the epigenetic basis of sheep fecundity. |
| first_indexed | 2024-12-24T11:08:51Z |
| format | Article |
| id | doaj.art-11b9ddfa239947ba8650860f95ac650a |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-24T11:08:51Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-11b9ddfa239947ba8650860f95ac650a2022-12-21T16:58:35ZengBMCReproductive Biology and Endocrinology1477-78272020-08-0118111110.1186/s12958-020-00633-9Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundityXiangyang Miao0Qingmiao Luo1Lingli Xie2Huijing Zhao3Xiaoyu Qin4State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesAbstract Background/aims Sheep are important livestock with variant ovulation rate and fertility. Dorset sheep is a typical breed with low prolificacy, whereas Small Tail Han sheep with FecB mutation (HanBB) have hyperprolificacy. Our previous studies have revealed the gene expression difference between the ovaries from Dorset and HanBB sheep contributes to the difference of fecundity, however, what leads to these gene expression difference remains unclear. DNA methylation, an important epigenetic process, plays a crucial role in gene expression regulation. Methods In the present study, we constructed a methylated DNA immunoprecipitation combined with high throughput sequencing (MeDIP-seq) strategy to investigate the differentially methylated genes between the Dorset and HanBB ovaries. Results Our findings suggest the genes involved in immune response, branched-chain amino acid metabolism, cell growth and cell junction were differentially methylated in or around the gene body regions. Conclusions These findings provide prospective insights on the epigenetic basis of sheep fecundity.http://link.springer.com/article/10.1186/s12958-020-00633-9SheepEpigeneticsOvaryMethylationFecundity |
| spellingShingle | Xiangyang Miao Qingmiao Luo Lingli Xie Huijing Zhao Xiaoyu Qin Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity Reproductive Biology and Endocrinology Sheep Epigenetics Ovary Methylation Fecundity |
| title | Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| title_full | Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| title_fullStr | Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| title_full_unstemmed | Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| title_short | Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| title_sort | comparative dna methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity |
| topic | Sheep Epigenetics Ovary Methylation Fecundity |
| url | http://link.springer.com/article/10.1186/s12958-020-00633-9 |
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