Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.)
Wucai (<i>Brassica campestris</i> L.) is a leafy vegetable that originated in China, its soluble sugars accumulate significantly to improve taste quality during maturation, and it is widely accepted by consumers. In this study, we investigated the soluble sugar content at different devel...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4816 |
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| author | Chenggang Wang Jiajie Zhou Shengnan Zhang Xun Gao Yitao Yang Jinfeng Hou Guohu Chen Xiaoyan Tang Jianqiang Wu Lingyun Yuan |
| author_facet | Chenggang Wang Jiajie Zhou Shengnan Zhang Xun Gao Yitao Yang Jinfeng Hou Guohu Chen Xiaoyan Tang Jianqiang Wu Lingyun Yuan |
| author_sort | Chenggang Wang |
| collection | DOAJ |
| description | Wucai (<i>Brassica campestris</i> L.) is a leafy vegetable that originated in China, its soluble sugars accumulate significantly to improve taste quality during maturation, and it is widely accepted by consumers. In this study, we investigated the soluble sugar content at different developmental stages. Two periods including 34 days after planting (DAP) and 46 DAP, which represent the period prior to and after sugar accumulation, respectively, were selected for metabolomic and transcriptomic profiling. Differentially accumulated metabolites (DAMs) were mainly enriched in the pentose phosphate pathway, galactose metabolism, glycolysis/gluconeogenesis, starch and sucrose metabolism, and fructose and mannose metabolism. By orthogonal projection to latent structures-discriminant s-plot (OPLS-DA S-plot) and MetaboAnalyst analyses, D-galactose and β-D-glucose were identified as the major components of sugar accumulation in wucai. Combined with the transcriptome, the pathway of sugar accumulation and the interact network between 26 DEGs and the two sugars were mapped. <i>CWINV4</i>, <i>CEL1</i>, <i>BGLU16</i>, and <i>BraA03g023380.3C</i> had positive correlations with the accumulation of sugar accumulation in wucai. The lower expression of <i>BraA06g003260.3C</i>, <i>BraA08g002960.3C</i>, <i>BraA05g019040.3C</i>, and <i>BraA05g027230.3C</i> promoted sugar accumulation during the ripening of wucai. These findings provide insights into the mechanisms underlying sugar accumulation during commodity maturity, providing a basis for the breeding of sugar-rich wucai cultivars. |
| first_indexed | 2024-03-11T07:21:14Z |
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| language | English |
| last_indexed | 2024-03-11T07:21:14Z |
| publishDate | 2023-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-11bb6bf22fb84935856bf5f2899ae7f92023-11-17T07:53:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245481610.3390/ijms24054816Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.)Chenggang Wang0Jiajie Zhou1Shengnan Zhang2Xun Gao3Yitao Yang4Jinfeng Hou5Guohu Chen6Xiaoyan Tang7Jianqiang Wu8Lingyun Yuan9College of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaCollege of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaWucai (<i>Brassica campestris</i> L.) is a leafy vegetable that originated in China, its soluble sugars accumulate significantly to improve taste quality during maturation, and it is widely accepted by consumers. In this study, we investigated the soluble sugar content at different developmental stages. Two periods including 34 days after planting (DAP) and 46 DAP, which represent the period prior to and after sugar accumulation, respectively, were selected for metabolomic and transcriptomic profiling. Differentially accumulated metabolites (DAMs) were mainly enriched in the pentose phosphate pathway, galactose metabolism, glycolysis/gluconeogenesis, starch and sucrose metabolism, and fructose and mannose metabolism. By orthogonal projection to latent structures-discriminant s-plot (OPLS-DA S-plot) and MetaboAnalyst analyses, D-galactose and β-D-glucose were identified as the major components of sugar accumulation in wucai. Combined with the transcriptome, the pathway of sugar accumulation and the interact network between 26 DEGs and the two sugars were mapped. <i>CWINV4</i>, <i>CEL1</i>, <i>BGLU16</i>, and <i>BraA03g023380.3C</i> had positive correlations with the accumulation of sugar accumulation in wucai. The lower expression of <i>BraA06g003260.3C</i>, <i>BraA08g002960.3C</i>, <i>BraA05g019040.3C</i>, and <i>BraA05g027230.3C</i> promoted sugar accumulation during the ripening of wucai. These findings provide insights into the mechanisms underlying sugar accumulation during commodity maturity, providing a basis for the breeding of sugar-rich wucai cultivars.https://www.mdpi.com/1422-0067/24/5/4816wucai (<i>Brassica campestris</i> L.)D-galactoseβ-D-glucosesugar accumulation pathwayinteract network |
| spellingShingle | Chenggang Wang Jiajie Zhou Shengnan Zhang Xun Gao Yitao Yang Jinfeng Hou Guohu Chen Xiaoyan Tang Jianqiang Wu Lingyun Yuan Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) International Journal of Molecular Sciences wucai (<i>Brassica campestris</i> L.) D-galactose β-D-glucose sugar accumulation pathway interact network |
| title | Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) |
| title_full | Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) |
| title_fullStr | Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) |
| title_full_unstemmed | Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) |
| title_short | Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai (<i>Brassica campestris</i> L.) |
| title_sort | combined metabolome and transcriptome analysis elucidates sugar accumulation in wucai i brassica campestris i l |
| topic | wucai (<i>Brassica campestris</i> L.) D-galactose β-D-glucose sugar accumulation pathway interact network |
| url | https://www.mdpi.com/1422-0067/24/5/4816 |
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