Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil
Healthcare-related infections caused by resistant microorganisms are a severe public health problem and are becoming increasingly prevalent in the hospital environment, especially Pseudomonas aeruginosa. This work aimed to evaluate the resistance profile of Pseudomonas aeruginosa to antimicrobials b...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2023-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100651&tlng=en |
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| author | G.P. Mesquita M.C.C. Costa M.A. Silva L.G. Araújo B.G. Vila Nova É.J.M. Castro L.C.M. Castelo Branco R.C.S. da Silva S.G. Marques A.G. Abreu |
| author_facet | G.P. Mesquita M.C.C. Costa M.A. Silva L.G. Araújo B.G. Vila Nova É.J.M. Castro L.C.M. Castelo Branco R.C.S. da Silva S.G. Marques A.G. Abreu |
| author_sort | G.P. Mesquita |
| collection | DOAJ |
| description | Healthcare-related infections caused by resistant microorganisms are a severe public health problem and are becoming increasingly prevalent in the hospital environment, especially Pseudomonas aeruginosa. This work aimed to evaluate the resistance profile of Pseudomonas aeruginosa to antimicrobials before the COVID-19 pandemic and during the pandemic period. Bacteria strains were obtained from tracheal aspiration, sputum, and bronchoalveolar lavage for diagnosis and phenotypic characterization. Matrix assisted laser-desorption ionization-time of flight mass spectrometry (MALD-TOF MS) was used to identify strains. Automated Phoenix and VITEK® 2 Compact system and the disc diffusion method were performed to determine the antimicrobial susceptibility profile. A total of 41,000 medical reports from adult patients with pneumonia were analyzed. Of these, 951 patients were positive for P. aeruginosa, of which 373 were related to the pre-pandemic period and 578 to the pandemic period. Older men (≥60 years) were more prevalent in both periods. P. aeruginosa strains were resistant to imipenem in both periods: 38.8 and 42.5%, respectively, followed by meropenem (34.2 and 39.2%), ciprofloxacin (33.6 and 36.7%), and levofloxacin (34.9 and 43.5%). Intensive care units had the highest percentage of affected patients (62 and 65%) compared with other sectors, with a prevalence of 71% in the public network before COVID-19 and 59% during the pandemic. Our data showed a prevalence of P. aeruginosa in elderly patients in both the pre-pandemic and pandemic periods. In addition, an increase in P. aeruginosa resistance to beta-lactams, quinolones, carbapenems, and cephalosporins was observed during the COVID-19 pandemic compared with the period before the pandemic, especially in ICUs. |
| first_indexed | 2024-03-12T22:00:41Z |
| format | Article |
| id | doaj.art-11bc2b8715d14e72a1ce0867a3b72956 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-12T22:00:41Z |
| publishDate | 2023-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-11bc2b8715d14e72a1ce0867a3b729562023-07-25T07:42:35ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2023-07-015610.1590/1414-431x2023e12726Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast BrazilG.P. Mesquitahttps://orcid.org/0000-0003-2611-7234M.C.C. Costahttps://orcid.org/0000-0003-4166-0278M.A. Silvahttps://orcid.org/0000-0001-6558-2863L.G. Araújohttps://orcid.org/0000-0002-4276-8689B.G. Vila Novahttps://orcid.org/0000-0001-5403-8520É.J.M. Castrohttps://orcid.org/0000-0001-8453-180XL.C.M. Castelo Brancohttps://orcid.org/0000-0002-8969-0129R.C.S. da Silvahttps://orcid.org/0000-0002-9284-3720S.G. Marqueshttps://orcid.org/0000-0003-3961-829XA.G. Abreuhttps://orcid.org/0000-0003-1665-0879Healthcare-related infections caused by resistant microorganisms are a severe public health problem and are becoming increasingly prevalent in the hospital environment, especially Pseudomonas aeruginosa. This work aimed to evaluate the resistance profile of Pseudomonas aeruginosa to antimicrobials before the COVID-19 pandemic and during the pandemic period. Bacteria strains were obtained from tracheal aspiration, sputum, and bronchoalveolar lavage for diagnosis and phenotypic characterization. Matrix assisted laser-desorption ionization-time of flight mass spectrometry (MALD-TOF MS) was used to identify strains. Automated Phoenix and VITEK® 2 Compact system and the disc diffusion method were performed to determine the antimicrobial susceptibility profile. A total of 41,000 medical reports from adult patients with pneumonia were analyzed. Of these, 951 patients were positive for P. aeruginosa, of which 373 were related to the pre-pandemic period and 578 to the pandemic period. Older men (≥60 years) were more prevalent in both periods. P. aeruginosa strains were resistant to imipenem in both periods: 38.8 and 42.5%, respectively, followed by meropenem (34.2 and 39.2%), ciprofloxacin (33.6 and 36.7%), and levofloxacin (34.9 and 43.5%). Intensive care units had the highest percentage of affected patients (62 and 65%) compared with other sectors, with a prevalence of 71% in the public network before COVID-19 and 59% during the pandemic. Our data showed a prevalence of P. aeruginosa in elderly patients in both the pre-pandemic and pandemic periods. In addition, an increase in P. aeruginosa resistance to beta-lactams, quinolones, carbapenems, and cephalosporins was observed during the COVID-19 pandemic compared with the period before the pandemic, especially in ICUs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100651&tlng=enDrug resistancePneumoniaPseudomonas aeruginosaCOVID-19 |
| spellingShingle | G.P. Mesquita M.C.C. Costa M.A. Silva L.G. Araújo B.G. Vila Nova É.J.M. Castro L.C.M. Castelo Branco R.C.S. da Silva S.G. Marques A.G. Abreu Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil Brazilian Journal of Medical and Biological Research Drug resistance Pneumonia Pseudomonas aeruginosa COVID-19 |
| title | Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil |
| title_full | Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil |
| title_fullStr | Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil |
| title_full_unstemmed | Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil |
| title_short | Antimicrobial resistance of Pseudomonas aeruginosa isolated from patients with pneumonia during the COVID-19 pandemic and pre-pandemic periods in Northeast Brazil |
| title_sort | antimicrobial resistance of pseudomonas aeruginosa isolated from patients with pneumonia during the covid 19 pandemic and pre pandemic periods in northeast brazil |
| topic | Drug resistance Pneumonia Pseudomonas aeruginosa COVID-19 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100651&tlng=en |
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