A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000
Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those inter...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2009-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0052 |
| _version_ | 1818757316911038464 |
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| author | Nalvo F. Almeida Shuangchun Yan Magdalen Lindeberg David J. Studholme David J. Schneider Bradford Condon Haijie Liu Carlos J. Viana Andrew Warren Clive Evans Eric Kemen Dan MacLean Aurelie Angot Gregory B. Martin Jonathan D. Jones Alan Collmer Joao C. Setubal Boris A. Vinatzer |
| author_facet | Nalvo F. Almeida Shuangchun Yan Magdalen Lindeberg David J. Studholme David J. Schneider Bradford Condon Haijie Liu Carlos J. Viana Andrew Warren Clive Evans Eric Kemen Dan MacLean Aurelie Angot Gregory B. Martin Jonathan D. Jones Alan Collmer Joao C. Setubal Boris A. Vinatzer |
| author_sort | Nalvo F. Almeida |
| collection | DOAJ |
| description | Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system–independent factors also play a role in this interaction. |
| first_indexed | 2024-12-18T06:09:00Z |
| format | Article |
| id | doaj.art-11bf0783f3824988b5977c9db38ad289 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:00Z |
| publishDate | 2009-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-11bf0783f3824988b5977c9db38ad2892022-12-21T21:18:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-01-01221526210.1094/MPMI-22-1-0052A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000Nalvo F. AlmeidaShuangchun YanMagdalen LindebergDavid J. StudholmeDavid J. SchneiderBradford CondonHaijie LiuCarlos J. VianaAndrew WarrenClive EvansEric KemenDan MacLeanAurelie AngotGregory B. MartinJonathan D. JonesAlan CollmerJoao C. SetubalBoris A. VinatzerDiverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system–independent factors also play a role in this interaction.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0052effector-triggered immunitynonhost resistancepyrosequencingSolexa sequencing |
| spellingShingle | Nalvo F. Almeida Shuangchun Yan Magdalen Lindeberg David J. Studholme David J. Schneider Bradford Condon Haijie Liu Carlos J. Viana Andrew Warren Clive Evans Eric Kemen Dan MacLean Aurelie Angot Gregory B. Martin Jonathan D. Jones Alan Collmer Joao C. Setubal Boris A. Vinatzer A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 Molecular Plant-Microbe Interactions effector-triggered immunity nonhost resistance pyrosequencing Solexa sequencing |
| title | A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 |
| title_full | A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 |
| title_fullStr | A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 |
| title_full_unstemmed | A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 |
| title_short | A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000 |
| title_sort | draft genome sequence of pseudomonas syringae pv tomato t1 reveals a type iii effector repertoire significantly divergent from that of pseudomonas syringae pv tomato dc3000 |
| topic | effector-triggered immunity nonhost resistance pyrosequencing Solexa sequencing |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-1-0052 |
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