Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification
The application of camptothecin (CPT) and its derivatives to control insect pests has generated significant interest. This study investigated the enzymatic response of cytochrome P450 monooxygenase (CYP) and glutathione S-transferase (GST) genes in the fat body cells of <i>Spodoptera exigua<...
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MDPI AG
2023-07-01
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| author | Zhenzhen Zhao Lan Zhang Yanning Zhang Liangang Mao Lizhen Zhu Xingang Liu Hongyun Jiang |
| author_facet | Zhenzhen Zhao Lan Zhang Yanning Zhang Liangang Mao Lizhen Zhu Xingang Liu Hongyun Jiang |
| author_sort | Zhenzhen Zhao |
| collection | DOAJ |
| description | The application of camptothecin (CPT) and its derivatives to control insect pests has generated significant interest. This study investigated the enzymatic response of cytochrome P450 monooxygenase (CYP) and glutathione S-transferase (GST) genes in the fat body cells of <i>Spodoptera exigua</i> after 10 μM CPT treatment. Additionally, we examined the effects of CPT on the growth and development of <i>S. exigua</i> larvae and detected the relative expression levels of selected CYP and GST genes during the CPT treatment period. Twenty-one CYP and 17 GST genes were identified from the fat body cells of <i>S. exigua</i> by comparative transcriptomic analyses. The relative expression of ten CYP and seven GST genes changed significantly, which suggested that these CPYs and GSTs may be involved in CPT metabolism. During exposure to CPT for 10 days, the development of <i>S. exigua</i> larvae was delayed and was characterized by weight inhibition and a prolonged period of development. The relative expression levels of the selected four CYP genes, <i>CYP9A27</i>, <i>CYP9A186</i>, <i>CYP337B5</i>, <i>CYP321A8</i>, and one GST gene, <i>GSTe7</i>, were significantly changed by CPT treatment compared to the control group. These generated data provide a basis for identifying the CPT metabolism/detoxification genes of <i>S. exigua</i> at the molecular level. |
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| spelling | doaj.art-11c164571d9646c6a765f0edcf5837802023-11-18T23:50:53ZengMDPI AGAgriculture2077-04722023-07-01138149410.3390/agriculture13081494Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin DetoxificationZhenzhen Zhao0Lan Zhang1Yanning Zhang2Liangang Mao3Lizhen Zhu4Xingang Liu5Hongyun Jiang6State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThe application of camptothecin (CPT) and its derivatives to control insect pests has generated significant interest. This study investigated the enzymatic response of cytochrome P450 monooxygenase (CYP) and glutathione S-transferase (GST) genes in the fat body cells of <i>Spodoptera exigua</i> after 10 μM CPT treatment. Additionally, we examined the effects of CPT on the growth and development of <i>S. exigua</i> larvae and detected the relative expression levels of selected CYP and GST genes during the CPT treatment period. Twenty-one CYP and 17 GST genes were identified from the fat body cells of <i>S. exigua</i> by comparative transcriptomic analyses. The relative expression of ten CYP and seven GST genes changed significantly, which suggested that these CPYs and GSTs may be involved in CPT metabolism. During exposure to CPT for 10 days, the development of <i>S. exigua</i> larvae was delayed and was characterized by weight inhibition and a prolonged period of development. The relative expression levels of the selected four CYP genes, <i>CYP9A27</i>, <i>CYP9A186</i>, <i>CYP337B5</i>, <i>CYP321A8</i>, and one GST gene, <i>GSTe7</i>, were significantly changed by CPT treatment compared to the control group. These generated data provide a basis for identifying the CPT metabolism/detoxification genes of <i>S. exigua</i> at the molecular level.https://www.mdpi.com/2077-0472/13/8/1494cytochrome P450 monooxygenaseglutathione S-transferasescamptothecin<i>Spodoptera exigua</i> |
| spellingShingle | Zhenzhen Zhao Lan Zhang Yanning Zhang Liangang Mao Lizhen Zhu Xingang Liu Hongyun Jiang Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification Agriculture cytochrome P450 monooxygenase glutathione S-transferases camptothecin <i>Spodoptera exigua</i> |
| title | Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification |
| title_full | Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification |
| title_fullStr | Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification |
| title_full_unstemmed | Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification |
| title_short | Transcriptome-Wide Identification of Cytochrome P450s and GSTs from <i>Spodoptera exigua</i> Reveals Candidate Genes Involved in Camptothecin Detoxification |
| title_sort | transcriptome wide identification of cytochrome p450s and gsts from i spodoptera exigua i reveals candidate genes involved in camptothecin detoxification |
| topic | cytochrome P450 monooxygenase glutathione S-transferases camptothecin <i>Spodoptera exigua</i> |
| url | https://www.mdpi.com/2077-0472/13/8/1494 |
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