Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663
<p>Abstract</p> <p>Background</p> <p>Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.</p> <p>Methods</p&g...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-04-01
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| Series: | Journal of Hematology & Oncology |
| Online Access: | http://www.jhoonline.org/content/4/1/20 |
| _version_ | 1819123018888118272 |
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| author | Zhuan Zhou Jian Wang Fang Tao Li Zhao Jian Pan Mei Liao Yan Wu Jian Ni |
| author_facet | Zhuan Zhou Jian Wang Fang Tao Li Zhao Jian Pan Mei Liao Yan Wu Jian Ni |
| author_sort | Zhuan Zhou |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.</p> <p>Methods</p> <p>Using bioinformatics and <it>in vitro </it>experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.</p> <p>Results</p> <p>Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies</p> |
| first_indexed | 2024-12-22T07:01:41Z |
| format | Article |
| id | doaj.art-11c1866eb32448d89cc28ea5360d6f18 |
| institution | Directory Open Access Journal |
| issn | 1756-8722 |
| language | English |
| last_indexed | 2024-12-22T07:01:41Z |
| publishDate | 2011-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Hematology & Oncology |
| spelling | doaj.art-11c1866eb32448d89cc28ea5360d6f182022-12-21T18:34:47ZengBMCJournal of Hematology & Oncology1756-87222011-04-01412010.1186/1756-8722-4-20Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663Zhuan ZhouJian WangFang TaoLi ZhaoJian PanMei LiaoYan WuJian Ni<p>Abstract</p> <p>Background</p> <p>Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.</p> <p>Methods</p> <p>Using bioinformatics and <it>in vitro </it>experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.</p> <p>Results</p> <p>Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies</p>http://www.jhoonline.org/content/4/1/20 |
| spellingShingle | Zhuan Zhou Jian Wang Fang Tao Li Zhao Jian Pan Mei Liao Yan Wu Jian Ni Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 Journal of Hematology & Oncology |
| title | Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 |
| title_full | Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 |
| title_fullStr | Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 |
| title_full_unstemmed | Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 |
| title_short | Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663 |
| title_sort | retinoic acid induces hl 60 cell differentiation via the upregulation of mir 663 |
| url | http://www.jhoonline.org/content/4/1/20 |
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