The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder
ObjectiveST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) encodes a type II membrane protein that is thought to catalyze the transfer of sialic acid (SA) from CMP-SA to N-linked oligosaccharides and glycoproteins. Some population and animal studies have indicated an association...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.929878/full |
| _version_ | 1811311829842919424 |
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| author | Xiaolei Yang Xiaolei Yang Lin Li Xuejiao Chai Jicheng Liu |
| author_facet | Xiaolei Yang Xiaolei Yang Lin Li Xuejiao Chai Jicheng Liu |
| author_sort | Xiaolei Yang |
| collection | DOAJ |
| description | ObjectiveST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) encodes a type II membrane protein that is thought to catalyze the transfer of sialic acid (SA) from CMP-SA to N-linked oligosaccharides and glycoproteins. Some population and animal studies have indicated an association between the ST8SIA2 gene and autism spectrum disorder (ASD). However, there is limited information on the correlation between ST8SIA2 and autistic behavioral symptoms.MethodsIn this study, 69 ASD and 76 normal control children who were age- and sex-matched were recruited. ST8SIA2 expression and methylation levels were measured by reverse transcription quantitative real-time PCR and pyrosequencing, respectively, and the behavioral phenotypes of ASD children were assessed.ResultsThe ASD group had lower ST8SIA2 gene expression levels than the control group [t(0.05/2,143) = 2.582, p = 0.011]. Moreover, ST8SIA2 expression levels were positively correlated with daily life skills (rs = 0.381, p = 0.008) and negatively associated with stereotyped behaviors in the ASD group (rs = -0.510, p = 0.004). The methylation levels of the Chr. 15: 92984625 and Chr. 15: 92998561 sites of the ST8SIA2 gene in ASD children were higher than those of controls. The Chr. 15: 92984625 site was positively correlated with the stereotyped behaviors of ASD children (rs = 0.41, p = 0.039).ConclusionThis study provides a scientific basis to elucidate the relationship between the ST8SIA2 gene and behavioral phenotypes of ASD. |
| first_indexed | 2024-04-13T10:25:52Z |
| format | Article |
| id | doaj.art-11c4836580ea47189d3bbaf7857d22ed |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-13T10:25:52Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-11c4836580ea47189d3bbaf7857d22ed2022-12-22T02:50:19ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-07-011610.3389/fnbeh.2022.929878929878The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorderXiaolei Yang0Xiaolei Yang1Lin Li2Xuejiao Chai3Jicheng Liu4Postdoctoral Workstation, Research Institute of Medical and Pharmacy, Qiqihar Medical University, Qiqihar, ChinaDepartment of Preventive Medicine, School of Public Health, Qiqihar Medical University, Qiqihar, ChinaCenter for Prevention of Disease, Hospital of Traditional Chinese Medicine of Qiqihar, Qiqihar, ChinaDepartment of Preventive Medicine, School of Public Health, Qiqihar Medical University, Qiqihar, ChinaPostdoctoral Workstation, Research Institute of Medical and Pharmacy, Qiqihar Medical University, Qiqihar, ChinaObjectiveST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) encodes a type II membrane protein that is thought to catalyze the transfer of sialic acid (SA) from CMP-SA to N-linked oligosaccharides and glycoproteins. Some population and animal studies have indicated an association between the ST8SIA2 gene and autism spectrum disorder (ASD). However, there is limited information on the correlation between ST8SIA2 and autistic behavioral symptoms.MethodsIn this study, 69 ASD and 76 normal control children who were age- and sex-matched were recruited. ST8SIA2 expression and methylation levels were measured by reverse transcription quantitative real-time PCR and pyrosequencing, respectively, and the behavioral phenotypes of ASD children were assessed.ResultsThe ASD group had lower ST8SIA2 gene expression levels than the control group [t(0.05/2,143) = 2.582, p = 0.011]. Moreover, ST8SIA2 expression levels were positively correlated with daily life skills (rs = 0.381, p = 0.008) and negatively associated with stereotyped behaviors in the ASD group (rs = -0.510, p = 0.004). The methylation levels of the Chr. 15: 92984625 and Chr. 15: 92998561 sites of the ST8SIA2 gene in ASD children were higher than those of controls. The Chr. 15: 92984625 site was positively correlated with the stereotyped behaviors of ASD children (rs = 0.41, p = 0.039).ConclusionThis study provides a scientific basis to elucidate the relationship between the ST8SIA2 gene and behavioral phenotypes of ASD.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.929878/fullautism spectrum disordersST8SIA2 geneDNA methylationbehavioral phenotypespyrosequencing |
| spellingShingle | Xiaolei Yang Xiaolei Yang Lin Li Xuejiao Chai Jicheng Liu The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder Frontiers in Behavioral Neuroscience autism spectrum disorders ST8SIA2 gene DNA methylation behavioral phenotypes pyrosequencing |
| title | The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder |
| title_full | The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder |
| title_fullStr | The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder |
| title_full_unstemmed | The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder |
| title_short | The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder |
| title_sort | association between st8sia2 gene and behavioral phenotypes in children with autism spectrum disorder |
| topic | autism spectrum disorders ST8SIA2 gene DNA methylation behavioral phenotypes pyrosequencing |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.929878/full |
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