The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin
DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is consi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8931 |
| _version_ | 1797523478900899840 |
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| author | Diane M. Spencer Angel Garza Reyna David S. Pisetsky |
| author_facet | Diane M. Spencer Angel Garza Reyna David S. Pisetsky |
| author_sort | Diane M. Spencer |
| collection | DOAJ |
| description | DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (<i>Micrococcus luteus,</i> calf thymus, <i>Escherichia</i><i>coli</i>, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and <i>E. coli</i> DNA showed binding with the monoclonal anti-DNA preparation. The specificity for Z-DNA conformation in MC DNA was demonstrated by an inhibition binding assay. An algorithm to identify propensity to form Z-DNA indicated that DNA from <i>Mycobacterium tuberculosis</i> could form Z-DNA, a prediction confirmed by immunoassay. Together, these findings indicate that anti-Z-DNA antibodies can serve as probes for the presence of Z-DNA in DNA of various species origin and that the content of Z-DNA varies significantly among DNA sources. |
| first_indexed | 2024-03-10T08:43:33Z |
| format | Article |
| id | doaj.art-11caea7f2d32483e8c29b215084487ce |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:43:33Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-11caea7f2d32483e8c29b215084487ce2023-11-22T08:02:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216893110.3390/ijms22168931The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species OriginDiane M. Spencer0Angel Garza Reyna1David S. Pisetsky2Department of Medicine and Immunology, Division of Rheumatology, Duke University Medical Center, Durham, NC 27710, USADepartment of Chemistry, Duke University, Durham, NC 27705, USADepartment of Medicine and Immunology, Division of Rheumatology, Duke University Medical Center, Durham, NC 27710, USADNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (<i>Micrococcus luteus,</i> calf thymus, <i>Escherichia</i><i>coli</i>, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and <i>E. coli</i> DNA showed binding with the monoclonal anti-DNA preparation. The specificity for Z-DNA conformation in MC DNA was demonstrated by an inhibition binding assay. An algorithm to identify propensity to form Z-DNA indicated that DNA from <i>Mycobacterium tuberculosis</i> could form Z-DNA, a prediction confirmed by immunoassay. Together, these findings indicate that anti-Z-DNA antibodies can serve as probes for the presence of Z-DNA in DNA of various species origin and that the content of Z-DNA varies significantly among DNA sources.https://www.mdpi.com/1422-0067/22/16/8931DNAZ-DNAB-DNAimmunoassayanti-DNA antibodiesconformation |
| spellingShingle | Diane M. Spencer Angel Garza Reyna David S. Pisetsky The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin International Journal of Molecular Sciences DNA Z-DNA B-DNA immunoassay anti-DNA antibodies conformation |
| title | The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin |
| title_full | The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin |
| title_fullStr | The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin |
| title_full_unstemmed | The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin |
| title_short | The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin |
| title_sort | binding of monoclonal and polyclonal anti z dna antibodies to dna of various species origin |
| topic | DNA Z-DNA B-DNA immunoassay anti-DNA antibodies conformation |
| url | https://www.mdpi.com/1422-0067/22/16/8931 |
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