Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients
The subgingival biofilm, as the most complex microbial community, has been proven to be reservoir of <i>Candida</i> spp. The main concept of this study was to investigate if there is a difference between the sensitivity of <i>Candida albicans</i> (<i>C. albicans</i&g...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/6/802 |
| _version_ | 1797490583410835456 |
|---|---|
| author | Milena Radunovic Milena Barac Jovana Kuzmanovic Pficer Dusan Pavlica Aleksandar Jovanovic Ana Pucar Sanja Petrovic |
| author_facet | Milena Radunovic Milena Barac Jovana Kuzmanovic Pficer Dusan Pavlica Aleksandar Jovanovic Ana Pucar Sanja Petrovic |
| author_sort | Milena Radunovic |
| collection | DOAJ |
| description | The subgingival biofilm, as the most complex microbial community, has been proven to be reservoir of <i>Candida</i> spp. The main concept of this study was to investigate if there is a difference between the sensitivity of <i>Candida albicans</i> (<i>C. albicans</i>) isolated from tongue and subgingival areas of periodontitis patients to antifungal agents. The aim of the study was to determine: (1) the distribution of different <i>Candida</i> species in the tongue and subgingival samples of periodontitis patients; (2) the susceptibility of <i>Candida albicans</i> strains from tongue and subgingival biofilm to the effects of commonly used antifungal agents: fluconazole, amphotericin B and itraconazole; (3) the correlation between the susceptibility of <i>Candida albicans</i> and clinical periodontal parameters. Tongue and subgingival biofilm samples of periodontitis subjects (N = 163) were examined. Susceptibility was tested when the same <i>Candida</i> species was isolated from both sites (17 subjects). <i>Candida</i> spp. were isolated in 23.3% of tongue and 21.5% of the subgingival samples. All isolates were susceptible to amphotericin B, while 64.71% of tongue and 52.94% of subgingival isolates were susceptible to fluconazole. A low frequency of itraconazole susceptibility was observed for tongue (17.64%) and subgingival isolates (11.76%). The correlations between full-mouth plaque score and Minimal Inhibitory Concentration (MIC) for tongue isolates were strongly positive for all antimycotics. Positive correlation was also observed between moderate periodontal destruction and MICs for tongue and subgingival isolates. The susceptibility of <i>C. albicans</i> to antifungals correlate with oral hygiene and moderate periodontal destruction. There is no difference in antifungal susceptibility between tongue and subgingival isolates. |
| first_indexed | 2024-03-10T00:35:03Z |
| format | Article |
| id | doaj.art-11cd183140ed4020bf6661b335549c92 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T00:35:03Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-11cd183140ed4020bf6661b335549c922023-11-23T15:17:36ZengMDPI AGAntibiotics2079-63822022-06-0111680210.3390/antibiotics11060802Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis PatientsMilena Radunovic0Milena Barac1Jovana Kuzmanovic Pficer2Dusan Pavlica3Aleksandar Jovanovic4Ana Pucar5Sanja Petrovic6Department of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, SerbiaDepartment of Oral Medicine and Periodontology, School of Dental Medicine, University of Belgrade, Dr Subotica 4, 11000 Belgrade, SerbiaDepartment for Medical Statistics and Informatics, School of Dental Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, SerbiaDepartment of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, SerbiaClinic of Urology, University Clinical Center of Serbia, Resavska 51, 11000 Belgrade, SerbiaDepartment of Oral Medicine and Periodontology, School of Dental Medicine, University of Belgrade, Dr Subotica 4, 11000 Belgrade, SerbiaDepartment of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, SerbiaThe subgingival biofilm, as the most complex microbial community, has been proven to be reservoir of <i>Candida</i> spp. The main concept of this study was to investigate if there is a difference between the sensitivity of <i>Candida albicans</i> (<i>C. albicans</i>) isolated from tongue and subgingival areas of periodontitis patients to antifungal agents. The aim of the study was to determine: (1) the distribution of different <i>Candida</i> species in the tongue and subgingival samples of periodontitis patients; (2) the susceptibility of <i>Candida albicans</i> strains from tongue and subgingival biofilm to the effects of commonly used antifungal agents: fluconazole, amphotericin B and itraconazole; (3) the correlation between the susceptibility of <i>Candida albicans</i> and clinical periodontal parameters. Tongue and subgingival biofilm samples of periodontitis subjects (N = 163) were examined. Susceptibility was tested when the same <i>Candida</i> species was isolated from both sites (17 subjects). <i>Candida</i> spp. were isolated in 23.3% of tongue and 21.5% of the subgingival samples. All isolates were susceptible to amphotericin B, while 64.71% of tongue and 52.94% of subgingival isolates were susceptible to fluconazole. A low frequency of itraconazole susceptibility was observed for tongue (17.64%) and subgingival isolates (11.76%). The correlations between full-mouth plaque score and Minimal Inhibitory Concentration (MIC) for tongue isolates were strongly positive for all antimycotics. Positive correlation was also observed between moderate periodontal destruction and MICs for tongue and subgingival isolates. The susceptibility of <i>C. albicans</i> to antifungals correlate with oral hygiene and moderate periodontal destruction. There is no difference in antifungal susceptibility between tongue and subgingival isolates.https://www.mdpi.com/2079-6382/11/6/802<i>Candida</i>antifungal agentsdrug resistanceantifungalperiodontitis |
| spellingShingle | Milena Radunovic Milena Barac Jovana Kuzmanovic Pficer Dusan Pavlica Aleksandar Jovanovic Ana Pucar Sanja Petrovic Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients Antibiotics <i>Candida</i> antifungal agents drug resistance antifungal periodontitis |
| title | Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients |
| title_full | Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients |
| title_fullStr | Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients |
| title_full_unstemmed | Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients |
| title_short | Antifungal Susceptibility of <i>Candida albicans</i> Isolated from Tongue and Subgingival Biofilm of Periodontitis Patients |
| title_sort | antifungal susceptibility of i candida albicans i isolated from tongue and subgingival biofilm of periodontitis patients |
| topic | <i>Candida</i> antifungal agents drug resistance antifungal periodontitis |
| url | https://www.mdpi.com/2079-6382/11/6/802 |
| work_keys_str_mv | AT milenaradunovic antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT milenabarac antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT jovanakuzmanovicpficer antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT dusanpavlica antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT aleksandarjovanovic antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT anapucar antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients AT sanjapetrovic antifungalsusceptibilityoficandidaalbicansiisolatedfromtongueandsubgingivalbiofilmofperiodontitispatients |