Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. Knowing the potential capacity of the virus to adapt to other species, the serological surveillance of SARS-CoV-2 infection in susceptible animals is important. Hong Ko...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/582 |
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| author | Yun Young Go Maura Carrai Yan Ru Choi Christopher J. Brackman Karina W. S. Tam Pierra Y. T. Law Fiona Woodhouse Jane Gray Ji Hun Kim Joohyung Park Chae Won Jeon Hyomi Jang Ioannis Magouras Nicola Decaro Samuel M. S. Cheng Malik Peiris Julia A. Beatty Vanessa R. Barrs |
| author_facet | Yun Young Go Maura Carrai Yan Ru Choi Christopher J. Brackman Karina W. S. Tam Pierra Y. T. Law Fiona Woodhouse Jane Gray Ji Hun Kim Joohyung Park Chae Won Jeon Hyomi Jang Ioannis Magouras Nicola Decaro Samuel M. S. Cheng Malik Peiris Julia A. Beatty Vanessa R. Barrs |
| author_sort | Yun Young Go |
| collection | DOAJ |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. Knowing the potential capacity of the virus to adapt to other species, the serological surveillance of SARS-CoV-2 infection in susceptible animals is important. Hong Kong and Seoul are two of Asia’s most densely populated urban cities, where companion animals often live in close contact with humans. Sera collected from 1040 cats and 855 dogs during the early phase of the pandemic in Hong Kong and Seoul were tested for SARS-CoV-2 antibodies using an ELISA that detects antibodies against the receptor binding domain of the viral spike protein. Positive sera were also tested for virus neutralizing antibodies using a surrogate virus neutralization (sVNT) and plaque reduction neutralization test (PRNT). Among feline sera, 4.51% and 2.54% of the samples from Korea and Hong Kong, respectively, tested ELISA positive. However, only 1.64% of the samples from Korea and 0.18% from Hong Kong tested positive by sVNT, while only 0.41% of samples from Korea tested positive by PRNT. Among canine samples, 4.94% and 6.46% from Korea and Hong Kong, respectively, tested positive by ELISA, while only 0.29% of sera from Korea were positive on sVNT and no canine sera tested positive by PRNT. These results confirm a low seroprevalence of SARS-CoV-2 exposure in companion animals in Korea and Hong Kong. The discordance between the RBD-ELISA and neutralization tests may indicate possible ELISA cross-reactivity with other coronaviruses, especially in canine sera. |
| first_indexed | 2024-03-11T07:59:23Z |
| format | Article |
| id | doaj.art-11cfc2707edc4bb58e5c7d3b25ca3594 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T07:59:23Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-11cfc2707edc4bb58e5c7d3b25ca35942023-11-16T23:50:58ZengMDPI AGViruses1999-49152023-02-0115258210.3390/v15020582Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and KoreaYun Young Go0Maura Carrai1Yan Ru Choi2Christopher J. Brackman3Karina W. S. Tam4Pierra Y. T. Law5Fiona Woodhouse6Jane Gray7Ji Hun Kim8Joohyung Park9Chae Won Jeon10Hyomi Jang11Ioannis Magouras12Nicola Decaro13Samuel M. S. Cheng14Malik Peiris15Julia A. Beatty16Vanessa R. Barrs17Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences and Centre for Animal Health and Welfare, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences and Centre for Animal Health and Welfare, City University of Hong Kong, Hong Kong 999077, ChinaAgriculture, Fisheries and Conservation Department, Government of the Hong Kong Special Administrative Region, Hong Kong 999077, ChinaAgriculture, Fisheries and Conservation Department, Government of the Hong Kong Special Administrative Region, Hong Kong 999077, ChinaAgriculture, Fisheries and Conservation Department, Government of the Hong Kong Special Administrative Region, Hong Kong 999077, ChinaSociety for the Prevention of Cruelty to Animals (Hong Kong), 5 Wan Shing St, Wan Chai, Hong Kong 999077, ChinaSociety for the Prevention of Cruelty to Animals (Hong Kong), 5 Wan Shing St, Wan Chai, Hong Kong 999077, China24 h Jamsil On Animal Medical Center, Songpa-gu, Seoul 05556, Republic of KoreaVIP Animal Medical Center, Seongbuk-gu, Seoul 02830, Republic of KoreaNeodin Biovet Laboratory, Guri-si 11956, Republic of KoreaNeodin Biovet Laboratory, Guri-si 11956, Republic of KoreaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, ItalySchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, ChinaSchool of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, ChinaDepartment of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences and Centre for Animal Health and Welfare, City University of Hong Kong, Hong Kong 999077, ChinaDepartment of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences and Centre for Animal Health and Welfare, City University of Hong Kong, Hong Kong 999077, ChinaSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. Knowing the potential capacity of the virus to adapt to other species, the serological surveillance of SARS-CoV-2 infection in susceptible animals is important. Hong Kong and Seoul are two of Asia’s most densely populated urban cities, where companion animals often live in close contact with humans. Sera collected from 1040 cats and 855 dogs during the early phase of the pandemic in Hong Kong and Seoul were tested for SARS-CoV-2 antibodies using an ELISA that detects antibodies against the receptor binding domain of the viral spike protein. Positive sera were also tested for virus neutralizing antibodies using a surrogate virus neutralization (sVNT) and plaque reduction neutralization test (PRNT). Among feline sera, 4.51% and 2.54% of the samples from Korea and Hong Kong, respectively, tested ELISA positive. However, only 1.64% of the samples from Korea and 0.18% from Hong Kong tested positive by sVNT, while only 0.41% of samples from Korea tested positive by PRNT. Among canine samples, 4.94% and 6.46% from Korea and Hong Kong, respectively, tested positive by ELISA, while only 0.29% of sera from Korea were positive on sVNT and no canine sera tested positive by PRNT. These results confirm a low seroprevalence of SARS-CoV-2 exposure in companion animals in Korea and Hong Kong. The discordance between the RBD-ELISA and neutralization tests may indicate possible ELISA cross-reactivity with other coronaviruses, especially in canine sera.https://www.mdpi.com/1999-4915/15/2/582SARS-CoV-2COVID-19felinecanineseroprevalencezoonosis |
| spellingShingle | Yun Young Go Maura Carrai Yan Ru Choi Christopher J. Brackman Karina W. S. Tam Pierra Y. T. Law Fiona Woodhouse Jane Gray Ji Hun Kim Joohyung Park Chae Won Jeon Hyomi Jang Ioannis Magouras Nicola Decaro Samuel M. S. Cheng Malik Peiris Julia A. Beatty Vanessa R. Barrs Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea Viruses SARS-CoV-2 COVID-19 feline canine seroprevalence zoonosis |
| title | Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea |
| title_full | Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea |
| title_fullStr | Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea |
| title_full_unstemmed | Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea |
| title_short | Low Prevalence of SARS-CoV-2 Antibodies in Canine and Feline Serum Samples Collected during the COVID-19 Pandemic in Hong Kong and Korea |
| title_sort | low prevalence of sars cov 2 antibodies in canine and feline serum samples collected during the covid 19 pandemic in hong kong and korea |
| topic | SARS-CoV-2 COVID-19 feline canine seroprevalence zoonosis |
| url | https://www.mdpi.com/1999-4915/15/2/582 |
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